Cloning and Expression of the Rhesus Monkey Oxytocin Receptor

Salvatore, Christopher; Woyden, Carla J.; Guidotti, Maribeth T.; Pettibone, Douglas J.; Jacobson, Marlene A.

doi:10.3109/10799899809039162

Cited by 16 publications

(4 citation statements)

References 14 publications

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“…Analogues in bold are agonists, all others are antagonists. References for analogues affinity: F‐180 (13); SR49059 (11); d(CH 2 )[DIle 2 ,Ile 4 ,Tyr 9 ‐NH 2 ]AVP (10); d(CH 2 ) 5 [Tyr(Me) 2 ]AVP (7, 8, 15); OT (8, 33); SR121463 (11); SSR149415 (21); d[Cha 4 ]AVP (22); [Thr 4 ,Gly 7 ]OT (32, 34); dDAVP (35); and L‐372 662 (36).…”

Section: Discussionmentioning

confidence: 99%

The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

Guillon

Derick

Peña

et al. 2004

J Neuroendocrinology

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Until recently, pharmacological studies dealing with vasopressin receptor isoforms were severely hampered by the lack of selective agonists or antagonists that recognize the pituitary V(1b) vasopressin receptor. By contrast, many selective vasopressin-related compounds are available for characterization of the vasopressor (V(1a)) or antidiuretic (V(2)) vasopressin receptor subtypes. Recently, SSR149415, a selective nonpeptide molecule, was discovered with nanomolar affinity for mammalian V(1b) receptors and good selectivity for the other vasopressin and oxytocin receptor isoforms. This molecule exhibits potent antagonist properties both in vitro and in vivo. We also designed synthetic peptides derived from [deaminocysteine(1),arginine(8)]vasopressin (dAVP), modified in position 4 by various amino acid residues. Some of these, d[cyclohexylalanine(4)]AVP or d[lysine(4)]AVP, have a high affinity and an excellent selectivity for the human V(1b) receptor subtype. However, they exhibit a mixed V(1b)/V(2) pharmacological profile for the rat vasopressin receptor isoforms. Whatever the species considered, these peptides behave as agonists both in bioassays performed in vitro and in vivo. The d[cyclohexylalanine(4)]AVP was tritiated and represents the first selective radiolabelled ligand available for studying the human V(1b) receptors. The discovery of these new selective V(1b) agonists and V(1b) antagonist allows an accurate pharmacological characterization of all the vasopressin receptor isoforms. As emphasized in this review, attention to the vasopressin and oxytocin receptor species differences is of critical importance in studies with all vasopressin and oxytocin ligands.

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Section: Discussionmentioning

confidence: 99%

The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

Guillon

Derick

Peña

et al. 2004

J Neuroendocrinology

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“…Two stably transfected Chinese hamster ovary (CHO) cell lines (gifts of Dr. Brian Roth, NIMH Psychoactive Drug Screening Program and UNC-Chapel Hill) were used. These cell lines served as proxies for rhesus receptors binding profiles, as the homology between the rhesus and human OXTR and AVPR1A amino acid sequences are high (>97%) (Salvatore et al, 1998; Young et al, 1999). Cells were cultured in 75 cm 2 flasks (Corning; Manassas, VA) in an incubator at 37°C, 100% humidity, 5% CO 2 , in sterile, filtered Ham's F-12 media (GIBCO; Grand Island, NY) with 10% fetal bovine serum (Hyclone; Waltham, MA), 1% Pen/Strep (GIBCO; Grand Island, NY), 15 mM 1X HEPES buffer solution (GIBCO; Grand Island, NY), and 400 μg/mL of the selection agent G418 sulfate (cellgro; Manassas, VA), which ensures growth of only the cells expressing the gene of interest.…”

Section: Methodsmentioning

confidence: 99%

The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta)

Freeman

Inoue

Smith

et al. 2014

Psychoneuroendocrinology

173

159

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The rhesus macaque (Macaca mulatta) is an important primate model for social cognition, and recent studies have begun to explore the impact of oxytocin on social cognition and behavior. Macaques have great potential for elucidating the neural mechanisms by which oxytocin modulates social cognition, which has implications for oxytocin-based pharmacotherapies for psychiatric disorders such as autism and schizophrenia. Previous attempts to localize oxytocin receptors (OXTR) in the rhesus macaque brain have failed due to reduced selectivity of radioligands, which in primates bind to both OXTR and the structurally similar vasopressin 1a receptor (AVPR1A). We have developed a pharmacologically-informed competitive binding autoradiography protocol that selectively reveals OXTR and AVPR1A binding sites in primate brain sections. Using this protocol, we describe the neuroanatomical distribution of OXTR in the macaque. Finally, we use in situ hybridization to localize OXTR mRNA. Our results demonstrate that OXTR expression in the macaque brain is much more restricted than AVPR1A. OXTR is largely limited to the nucleus basalis of Meynert, pedunculopontine tegmental nucleus, the superficial gray layer of the superior colliculus, the trapezoid body, and the ventromedial hypothalamus. These regions are involved in a variety of functions relevant to social cognition, including modulating visual attention, processing auditory and multimodal sensory stimuli, and controlling orienting responses to visual stimuli. These results provide insights into the neural mechanisms by which oxytocin modulates social cognition and behavior in this species, which, like humans, uses vision and audition as the primary modalities for social communication.

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“…The OT receptor has been cloned from human (273), rat (440), and other species (43,190,284,427,451). The OT receptor is highly conserved across species and has been found in a variety of tissues, particularly in uterus but also in mammary gland, pituitary, brain, kidney, thymus, ovary, testis, heart, and blood vessels.…”

Section: Oxytocin Receptorsmentioning

confidence: 99%

Neuroendocrine Control of Body Fluid Metabolism

Antunes‐Rodrigues

Castro

Elias

et al. 2004

Physiological Reviews

401

310

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Mammals control the volume and osmolality of their body fluids from stimuli that arise from both the intracellular and extracellular fluid compartments. These stimuli are sensed by two kinds of receptors: osmoreceptor-Na+ receptors and volume or pressure receptors. This information is conveyed to specific areas of the central nervous system responsible for an integrated response, which depends on the integrity of the anteroventral region of the third ventricle, e.g., organum vasculosum of the lamina terminalis, median preoptic nucleus, and subfornical organ. The hypothalamo-neurohypophysial system plays a fundamental role in the maintenance of body fluid homeostasis by secreting vasopressin and oxytocin in response to osmotic and nonosmotic stimuli. Since the discovery of the atrial natriuretic peptide (ANP), a large number of publications have demonstrated that this peptide provides a potent defense mechanism against volume overload in mammals, including humans. ANP is mostly localized in the heart, but ANP and its receptor are also found in hypothalamic and brain stem areas involved in body fluid volume and blood pressure regulation. Blood volume expansion acts not only directly on the heart, by stretch of atrial myocytes to increase the release of ANP, but also on the brain ANPergic neurons through afferent inputs from baroreceptors. Angiotensin II also plays an important role in the regulation of body fluids, being a potent inducer of thirst and, in general, antagonizes the actions of ANP. This review emphasizes the role played by brain ANP and its interaction with neurohypophysial hormones in the control of body fluid homeostasis.

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Cloning and Expression of the Rhesus Monkey Oxytocin Receptor

Cited by 16 publications

References 14 publications

The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta)

Neuroendocrine Control of Body Fluid Metabolism

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Cloning and Expression of the Rhesus Monkey Oxytocin Receptor

Cited by 16 publications

References 14 publications

The Discovery of Novel Vasopressin V1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

The Discovery of Novel Vasopressin V1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta)

Neuroendocrine Control of Body Fluid Metabolism

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Product

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The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations

The Discovery of Novel Vasopressin V_1b Receptor Ligands for Pharmacological, Functional and Structural Investigations