Cloning and Expression of the Cutinase A Gene of <i>Botrytis cinerea</i>

Vlugt-Bergmans, C.J.B. van der; Wagemakers, C.A.M.; Kan, Jan A.L. van

doi:10.1094/mpmi.1997.10.1.21

Cited by 81 publications

(50 citation statements)

References 25 publications

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“…cutA has previously been shown to be induced in B. cinerea mycelium in the presence of cutin monomers and repressed by glucose (48,61,62). We found that cutA and cutB were induced during germination with different kinetics (Fig.…”

Section: Discussionsupporting

confidence: 50%

Transcriptome Profiling of Botrytis cinerea Conidial Germination Reveals Upregulation of Infection-Related Genes during the Prepenetration Stage

et al. 2013

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Botrytis cinerea causes gray mold on a great number of host plants. Infection is initiated by airborne conidia that invade the host tissue, often by penetration of intact epidermal cells. To mimic the surface properties of natural plant surfaces, conidia were incubated on apple wax-coated surfaces, resulting in rapid germination and appressorium formation. Global changes in gene expression were analyzed by microarray hybridization between conidia incubated for 0 h (dormant), 1 h (pregermination), 2.5 h (postgermination), 4 h (appressoria), and 15 h (early mycelium). Considerable changes were observed, in particular between 0 h and 1 h. Genes induced during germination were enriched in those genes encoding secreted proteins, including lytic enzymes. Comparison of wild-type and a nonpathogenic MAP kinase mutant (bmp1) revealed marked differences in germination-related gene expression, in particular related to secretory proteins. Using promoter-GFP reporter strains, we detected a strictly germination-specific expression pattern of a putative chitin deacetylase gene (cda1). In contrast, a cutinase gene (cutB) was found to be expressed only in the presence of plant lipids, in a developmentally less stringent pattern. We also identified a coregulated gene cluster possibly involved in secondary metabolite synthesis which was found to be controlled by a transcription factor also encoded in this cluster. Our data demonstrate that early conidial development in B. cinerea is accompanied by rapid shifts in gene expression that prepare the fungus for germ tube outgrowth and host cell invasion.

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Section: Discussionsupporting

confidence: 50%

Transcriptome Profiling of Botrytis cinerea Conidial Germination Reveals Upregulation of Infection-Related Genes during the Prepenetration Stage

et al. 2013

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“…This includes the ability of the fungus to penetrate the leaf surface, relevant to this study as plants were not wounded prior to inoculation, and possibly the extent of plant defense activation by attempted fungal penetration of the leaf surface. However, the loss of penetration-related enzymes, such as cutinase, or the ability to form penetration structures, such as appressoria, have not impaired B. cinerea pathogenicity in experimental settings van Kan et al 1997;Gourgues et al 2004). Loci associated with A. thaliana ''penetration'' mutants, whose defects allow inappropriate penetration of A. thaliana leaves by non-Arabidopsis pathogens, are not colocalized with B. cinerea early lesion QTL detected in this study (Collins et al 2003;Assaad et al 2004;Stein et al 2006).…”

Section: Discussionmentioning

confidence: 51%

Complex Genetics Control Natural Variation inArabidopsis thalianaResistance toBotrytis cinerea

2008

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The genetic architecture of plant defense against microbial pathogens may be influenced by pathogen lifestyle. While plant interactions with biotrophic pathogens are frequently controlled by the action of large-effect resistance genes that follow classic Mendelian inheritance, our study suggests that plant defense against the necrotrophic pathogen Botrytis cinerea is primarily quantitative and genetically complex. Few studies of quantitative resistance to necrotrophic pathogens have used large plant mapping populations to dissect the genetic structure of resistance. Using a large structured mapping population of Arabidopsis thaliana, we identified quantitative trait loci influencing plant response to B. cinerea, measured as expansion of necrotic lesions on leaves and accumulation of the antimicrobial compound camalexin. Testing multiple B. cinerea isolates, we identified 23 separate QTL in this population, ranging in isolatespecificity from being identified with a single isolate to controlling resistance against all isolates tested. We identified a set of QTL controlling accumulation of camalexin in response to pathogen infection that largely colocalized with lesion QTL. The identified resistance QTL appear to function in epistatic networks involving three or more loci. Detection of multilocus connections suggests that natural variation in specific signaling or response networks may control A. thaliana-B. cinerea interaction in this population.

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“…Recent results obtained with cutinase gene-disrupted mutants of Magnaporte grisea [5], F. solani [6^8], Alternaria brassicicola [9] and Botrytis cinerea [10,11] have not con¢rmed this concept of a dual cutinase function. Although virulence penalties reported for a gene-disrupted mutant of F. solani remain controversial [7,12], the disruption of cutinase genes strongly expressed during saprophytic growth of the pathogens on cutin had no apparent e¡ect on the pathogenicity of respective mutants.…”

Section: Introductionmentioning

confidence: 99%

Diversity of cutinases from plant pathogenic fungi: differential and sequential expression of cutinolytic esterases by Alternaria brassicicola

Chen

1998

FEMS Microbiology Letters

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Plant cuticles provide a protective layer that has to be penetrated by fungal pathogens. Evidence is provided for a differential and sequential induction of two classes of cutinolytic esterases by Alternaria brassicicola. Serine esterases with cutinolytic activities were expressed by conidia germinating on host surfaces. The enzymes were not induced by surface wax or cutin monomers. They were only expressed during initial (24 h) contact of conidia with cutin on host surfaces freed from wax, and with cutin in aqueous suspensions. In contrast, contact with cutin had no immediate effect on the expression of CUTAB1, a gene encoding two cutinase isozymes with crucial functions in the saprophytic utilization of cutin. Presence of a cutin monomer or prolonged exposure to cutin was required for the induction of CUTAB1 expression. The differential induction of cutinolytic esterases indicates a sequential recognition of cutin as a barrier to be penetrated and to be utilized as a carbon source in saprophytic stages. ß 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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Cloning and Expression of the Cutinase A Gene of Botrytis cinerea

Cited by 81 publications

References 25 publications

Transcriptome Profiling of Botrytis cinerea Conidial Germination Reveals Upregulation of Infection-Related Genes during the Prepenetration Stage

Transcriptome Profiling of Botrytis cinerea Conidial Germination Reveals Upregulation of Infection-Related Genes during the Prepenetration Stage

Complex Genetics Control Natural Variation inArabidopsis thalianaResistance toBotrytis cinerea

Diversity of cutinases from plant pathogenic fungi: differential and sequential expression of cutinolytic esterases by Alternaria brassicicola

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