Cloning and expression of yellowtail ascites virus segment A

Abstract

cDNA of yellowtail ascites virus (YAV) segment A encoding a polyprotein of VP2, NS, and VP3 has been cloned. Comparison of the nucleotide and the deduced amino acid sequences showed very high homology between YAV and other aquatic birnaviruses. The two small open reading frames (VP5) besides the 5' terminus of the VP2 gene were found on segment A of YAV. Proteins encoded by cDNAs from segment A and the serotype-specific epitope region on VP2 were expressed using a baculovirus vector. Western blot analysis confirmed that a polyprotein was expressed and processed into VP2 and VP3 in insect cells infected with the recombinant baculovirus containing the complete polyprotein coding region. In the case of expression in silkworm larvae, only VP3 was detected in hemocytes and fat body of silkworm larvae infected with the recombinant virus. The recombinant fusion protein consisting of VP2 epitope region and polyhedrin was expressed in insect cells and cross-reacted with a mouse monoclonal antibody against VP2 which had a neutralizing activity to YAV.

Infectious pancreatic necrosis virus in salmonids: Molecular epidemiology and host response to infection

“…MABVs) including isolates belonging to the species YTAV. 29,38 It is important to emphasise that there have been discrepancies in the numbering of the different IPNV genogroups, with several authors numbering the genogroups differently than that proposed by Blake et al. 27 Moreover, Blake et al 27 swapped the numbers of Genogroups 2, 3, and 4 in the dendrogram of their publication and later an errata with the correct numbering was published.…”

“…Importantly, this genogroup was comprised of only Japanese aquabirnaviruses isolated from marine shellfish and fish (i.e. MABVs) including isolates belonging to the species YTAV 29,38 …”