Cloning and expression pattern of a hemolin homologue from the diamondback moth, Plutella xylostella

Kim, Rye Jin; Jo, Yong Hun; Oh, Seung Han; Park, Cheol Hong; Noh, Mi Young; Lee, Hyo‐Jeong; Kim, Seongon; Lee, Yong Seok; Kim, Iksoo

doi:10.1007/s13258-010-0834-7

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Plutella xylostella larvae were reared on Chinese cabbage leaf in an environmental chamber at 25 ± 1°C with 60 ± 5% relative humidity and a 16 h light : 8 h dark cycle (Kim et al . 2010).…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Kim

Noh

et al. 2010

Entomological Research

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Calreticulin (CRT) plays pivotal roles in Ca2+ homeostasis, molecular chaperoning, infection, inflammation and innate immunity. In an attempt to study the involvement of CRT in innate immunity, the full‐length cDNA of calreticulin (PxCRT) was cloned from the diamondback moth, Plutella xylostella. It consists of 1674 bp (excluding poly‐A tail) with a longest open reading frame (ORF) of 1197 bp encoding 398 amino acids. In silico analysis of PxCRT ORF reveals that it has various repeat motifs and endoplasmic reticulum retention signal found in all the calreticulin proteins. As expected, high amino acid sequence identities were found from other CRTs identified from Bombyx mori (87%), Galleria mellonella (87%), Apis mellifera (74%), Anopheles gambiae (74%), Tribolium castaneum (73%), Culex quinquefasciatus (73%), Rhodnius prolixus (72%), Nasonia vitripennis (71%), Drosophila melanogaster (71%) and Haemaphysalis qinghaiensis (68%). During development, P. xylostella expressed PxCRT predominantly in the pupal stage. In addition, spatial expression pattern analysis indicates that PxCRT was highly expressed in the silk gland. PxCRT mRNA, furthermore, was strongly induced 3 to 6 h after laminarin treatment, suggesting that PxCRT appears to be involved in immune responses and also plays an important role in the silk gland.

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Section: Methodsmentioning

confidence: 99%

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Kim

Noh

et al. 2010

Entomological Research

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“…These results suggest the communication of genetic information between different populations of Indian M. vitrata. A lack of genetic structure due to high migration rate has been demonstrated in several other insect species, including Plutella xylostella from China [8,62], Korea [63], USA [64,65], and Oceania (Australia and New Zealand) [66]; Mythimna separata from China [67]; Chrysodeixis includes from Brazil [56]; and Argyresthia conjugella from the Scandinavian Peninsula [68].…”

Section: Discussionmentioning

confidence: 99%

De Novo Mining and Validating Novel Microsatellite Markers to Assess Genetic Diversity in Maruca vitrata (F.), a Legume Pod Borer

Mahalle

Bosamia

Chakravarty

et al. 2023

Genes

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Maruca vitrata (Fabricius) is an invasive insect pest capable of causing enormous economic losses to a broad spectrum of leguminous crops. Microsatellites are valuable molecular markers for population genetic studies; however, an inadequate number of M. vitrata microsatellite loci are available to carry out population association studies. Thus, we utilized this insect’s public domain databases for mining expressed sequence tags (EST)-derived microsatellite markers. In total, 234 microsatellite markers were identified from 10053 unigenes. We discovered that trinucleotide repeats were the most predominant microsatellite motifs (61.53%), followed by dinucleotide repeats (23.50%) and tetranucleotide repeats (14.95%). Based on the analysis, twenty-five markers were selected for validation in M. vitrata populations collected from various regions of India. The number of alleles (Na), observed heterozygosity (Ho), and expected heterozygosity (He) ranged from 2 to 5; 0.00 to 0.80; and 0.10 to 0.69, respectively. The polymorphic loci showed polymorphism information content (PIC), ranging from 0.09 to 0.72. Based on the genetic distance matrix, the unrooted neighbor-joining dendrogram differentiated the selected populations into two discrete groups. The SSR markers developed and validated in this study will be helpful in population-level investigations of M. vitrata to understand the gene flow, demography, dispersal patterns, biotype differentiation, and host dynamics.

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“…In addition, other genes that may play important roles in immune defense in moths also have been studied by using RNAi techniques, such as the translationally controlled tumor protein in B. mori [111], hemolin homologue in P. xylostella [112] and H. cecropia [47], apolipophorin III in P. xylostella [113], protein kinase C alpha binding protein in S. exigua [113], and the GAPDH in P. xylostella [40]. …”

Section: Use Of Rnai For Gene Function Analysis In Mothsmentioning

confidence: 99%

RNA Interference in Moths: Mechanisms, Applications, and Progress

Wang

Chen

et al. 2016

Genes

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The vast majority of lepidopterans, about 90%, are moths. Some moths, particularly their caterpillars, are major agricultural and forestry pests in many parts of the world. However, some other members of moths, such as the silkworm Bombyx mori, are famous for their economic value. Fire et al. in 1998 initially found that exogenous double-stranded RNA (dsRNA) can silence the homolog endogenous mRNA in organisms, which is called RNA interference (RNAi). Soon after, the RNAi technique proved to be very promising not only in gene function determination but also in pest control. However, later studies demonstrate that performing RNAi in moths is not as straightforward as shown in other insect taxa. Nevertheless, since 2007, especially after 2010, an increasing number of reports have been published that describe successful RNAi experiments in different moth species either on gene function analysis or on pest management exploration. So far, more than 100 peer-reviewed papers have reported successful RNAi experiments in moths, covering 10 families and 25 species. By using classic and novel dsRNA delivery methods, these studies effectively silence the expression of various target genes and determine their function in larval development, reproduction, immunology, resistance against chemicals, and other biological processes. In addition, a number of laboratory and field trials have demonstrated that RNAi is also a potential strategy for moth pest management. In this review, therefore, we summarize and discuss the mechanisms and applications of the RNAi technique in moths by focusing on recent progresses.

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Cloning and expression pattern of a hemolin homologue from the diamondback moth, Plutella xylostella

Cited by 6 publications

References 25 publications

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

De Novo Mining and Validating Novel Microsatellite Markers to Assess Genetic Diversity in Maruca vitrata (F.), a Legume Pod Borer

RNA Interference in Moths: Mechanisms, Applications, and Progress

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