Cloning and expression patterns of the brine shrimp (Artemia sinica) glycogen phosphorylase (GPase) gene during development and in response to temperature stress

Zhao, Ning; Hou, Ming‐Feng; Wang, Ting; Chen, Yifei; Lv, Ying; Li, Zengrong; Zhang, Rui; Xin, Wenting; Zou, Xiangyang; Lin, Hui

doi:10.1007/s11033-013-2764-3

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Glycogen phosphorylase and α-amylase, which break down polysaccharides into monosaccharides, were up-regulated. These two proteins are also up-regulated in various species exposed to LT stress [56–58]. Moreover, some enzymes related to polysaccharides biosynthesis (maltooligosyl trehalose synthase, 1,4-alpha-glucan branching enzyme, glycogen synthase) underwent a decrease in levels.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Chang

Sun

et al. 2016

PLoS ONE

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Low temperature (LT) is one of the most important abiotic stresses that can significantly reduce crop yield. To gain insight into how Spirulina responds to LT stress, comprehensive physiological and proteomic analyses were conducted in this study. Significant decreases in growth and pigment levels as well as excessive accumulation of compatible osmolytes were observed in response to LT stress. An isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach was used to identify changes in protein abundance in Spirulina under LT. A total of 3,782 proteins were identified, of which 1,062 showed differential expression. Bioinformatics analysis indicated that differentially expressed proteins that were enriched in photosynthesis, carbohydrate metabolism, amino acid biosynthesis, and translation are important for the maintenance of cellular homeostasis and metabolic balance in Spirulina when subjected to LT stress. The up-regulation of proteins involved in gluconeogenesis, starch and sucrose metabolism, and amino acid biosynthesis served as coping mechanisms of Spirulina in response to LT stress. Moreover, the down-regulated expression of proteins involved in glycolysis, TCA cycle, pentose phosphate pathway, photosynthesis, and translation were associated with reduced energy consumption. The findings of the present study allow a better understanding of the response of Spirulina to LT stress and may facilitate in the elucidation of mechanisms underlying LT tolerance.

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Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Chang

Sun

et al. 2016

PLoS ONE

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“…Note that this normalization was also used to generate the 2D STOCSY in Figure S11. In Figure C, the carbohydrates still point down which indicates they are being used faster than the lipids, consistent with carbohydrates being the most readily available source of energy. − Conversely, a plethora of signals point upward indicating that relative to lipids they are increasing in concentration. The majority of these are consistent with the full range of amino acids (see Figure S12), confirming that much of the early energy investments by the organisms are to convert carbohydrates/lipids into amino acids, likely for protein synthesis.…”

Section: Resultsmentioning

confidence: 69%

Development of a Simple Cost Effective Oxygenation System for In Vivo Solution State NMR in 10 mm NMR Tubes

Costa,

Lysak,

Soong

et al. 2024

Anal. Chem.

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In vivo NMR is evolving into an important tool to understand biological processes and environmental responses. Current approaches use flow systems to sustain the organisms with oxygenated water and food (e.g., algae) inside the NMR. However, such systems have the potential to leak and clog (potentially damaging costly hardware), require large volumes of media, and multiple expensive HPLC pumps. The proposed "oxygenation system", uses a simple "double slit" adapter and a single air/oxygen flow line into the NMR. The design is especially suited to larger diameter probes given that standard flow systems would require higher flow rates thus amplifying the potential and impact of leaks/clogs. Traditionally, in vivo NMR of small organisms (e.g., Daphnia) have required 2D NMR in combination with 13 C enrichment to overcome susceptibility distortions and provide information rich metabolic profiles. Here Daphnia magna, Eisenia fetida and Artemia franciscana are used to demonstrate the potential of the oxygenation system. Survivability tests and 1 H time-resolved monitoring were first performed on D. magna, while E. fetida contained enough biomass to permit 1 H− 13 C HSQC, 13 C− 1 H HETCOR and 31 P NMR without isotopic enrichment. Finally, STOCSY of 1D 13 C NMR was used to follow the growth of A. franciscana (without 13 C enrichment) for 48 h after birth, which helps visualize trends across a series of 1D in vivo data. In summary, application of the oxygenation system toward larger diameter probes allows the collection of NMR data without enrichment, offering a promising solution to better understand processes in vivo.

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“…Glycogen participates in macromolecular synthesis as a metabolic reserve in animals. Glycogen phosphorylase (GP) is a key enzyme involved in intracellular glycogen catabolism and catalyzes the first step in glycogen degradation [ 53 ]. Glycogen phosphorylase-like ( GPL ) showed a down-regulated expression pattern in the experimental group in this study, it was hypothesized that Lvit- GIH2 had an inhibitory effect on GPL .…”

Section: Discussionmentioning

confidence: 99%

A new type II CHH neuropeptide involves ovarian development in the peppermint shrimp, Lysmata vittata

Lan,

Liu,

et al. 2024

PLoS ONE

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Type II crustacean hyperglycemic hormone (CHH) neuropeptides play diverse roles in crustaceans. In the hermaphrodite shrimp Lysmata vittata, two transcripts of type II CHHs (molt-inhibiting hormone/gonad-inhibiting hormone, MIH/GIH1 and MIH/GIH2) were identified by transcriptome sequencing, and MIH/GIH1 was later named Lvit-GIH1 for its inhibitory effect on ovarian development. Based on the high similarity of MIH/GIH2 to Lvit-GIH1, we named tentatively MIH/GIH2 as Lvit-GIH2 and explored the role of Lvit-GIH2 in ovarian development. The open reading frame (ORF) of Lvit-GIH2 was 333 bp in length, encoding a precursor consisted of a 32-aa signal peptide and a 78-aa mature peptide, which shared high sequence similarity with the type II subfamily peptides in crustaceans. Notably, Lvit-GIH2 was widely expressed in multiple tissues. The qRT-PCR findings indicated a rising trend in the expression of Lvit-GIH2 from the male phase to the euhermaphrodite phase. Both RNA interference and addition of GIH2 recombinant proteins (rGIH2) experiments showed that Lvit-GIH2 suppressed Lvit-Vg expression in hepatopancreas and Lvit-VgR expression in ovary. To further investigate the role of Lvit-GIH2 in ovarian development, the RNA-sequence analysis was performed to examine the changes in ovary after addition of rGIH2. The results showed that the pathways (Cysteine and methionine metabolism, Apoptosis-multiple species, etc.) and the genes (17bHSD8, IGFR, CHH, etc.) related to ovarian development were negatively regulated by rGIH2. In brief, Lvit-GIH2 might inhibit the ovarian development in L. vittata.

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Cloning and expression patterns of the brine shrimp (Artemia sinica) glycogen phosphorylase (GPase) gene during development and in response to temperature stress

Cited by 9 publications

References 27 publications

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Development of a Simple Cost Effective Oxygenation System for In Vivo Solution State NMR in 10 mm NMR Tubes

A new type II CHH neuropeptide involves ovarian development in the peppermint shrimp, Lysmata vittata

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