Cloning and expression profile of 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene from an oil-bearing rose

Wang, H.; Yao, Lu

doi:10.1134/s1021443714040207

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Fourteen enzymes are involved in the first phase of terpenoid biosynthesis, namely AACT HMGS, HMGR, mevalonate kinase (MVK), phosphomevalonate kinase (PMK), and mevalonate diphosphate decarboxylase (MVD), isopentenyl diphosphateisomerase (IDI), 1-deoxy- d -xylulose 5-phosphate synthase (DXS), 1-deoxy- d -xylulose 5-phosphate reductoisomerase (DXR), 2-C-methyl- d -erythritol 4-phosphate cytidylyltransferase (MCT), 4-(cytidine 50-diphospho)-2-C-methyl- d -erythritol kinase (CMK), 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase (MDS), ( E )-4-hydroxy-3-methyl-but-2-enyldiphosphate synthase (HDS), ( E )-4-hydroxy-3-methyl-but-2-enyl diphosphatereductase (HDR). Studies have suggested that these enzymes could affect the yield of essential oil [38,39]. Therefore, we should focus on expression change of these enzymes.…”

Section: Discussionmentioning

confidence: 99%

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

et al. 2016

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Abstract:Mountain pepper (Litsea cubeba (Lour.) Pers.) (Lauraceae) is an important industrial crop as an ingredient in cosmetics, pesticides, food additives and potential biofuels. These properties are attributed to monoterpenes and sesquiterpenes. However, there is still no integrated model describing differentially expressed genes (DEGs) involved in terpenoid biosynthesis during the fruit development of L. cubeba. Here, we performed digital gene expression (DGE) using the Illumina NGS platform to evaluated changes in gene expression during fruit development in L. cubeba. DGE generated expression data for approximately 19354 genes. Fruit at 60 days after flowering (DAF) served as the control, and a total of 415, 1255, 449 and 811 up-regulated genes and 505, 1351, 1823 and 1850 down-regulated genes were identified at 75, 90, 105 and 135 DAF, respectively. Pathway analysis revealed 26 genes involved in terpenoid biosynthesis pathways. Three DEGs had continued increasing or declining trends during the fruit development. The quantitative real-time PCR (qRT-PCR) results of five differentially expressed genes were consistent with those obtained from Illumina sequencing. These results provide a comprehensive molecular biology background for research on fruit development, and information that should aid in metabolic engineering to increase the yields of L. cubeba essential oil.

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Section: Discussionmentioning

confidence: 99%

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

et al. 2016

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Cloning, characterization, and expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene from Dioscorea zingiberensis

Cheng¹,

Wang²,

Shen³

et al. 2019

Biol. plant.

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Diosgenin, mainly extracted from some Dioscorea species, is the most important starting material for the production of steroidal drugs. It is believed that diosgenin in Dioscorea is synthesized from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP, the isomer of IPP) produced by the cytosolic mevalonate pathway. So far, the possibility of the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for biosynthesis of diosgenin remains unclear. The key enzyme in the MEP pathway is 1-deoxy-D-xylulose 5-phosphate-reductoisomerase (DXR, EC: 1.1.1.267). In this study, a DXR gene, named DzDXR (GenBank accession number KY131955), was isolated from Dioscorea zingiberensis. The DzDXR has an open reading frame of 1 413 bp encoding a protein of 470 amino acid residues. The function of DzDXR was verified by a colour enhancement assay in the Escherichia coli cells harbouring the plasmid pAC-BETA. Bioinformatic analyses revealed that DzDXR had a putative plastid transit peptide at the N-terminal region and was highly homologous to other plant DXRs, especially to those in monocotyledons. During the growth period of D. zingiberensis, the expression of DzDXR was found significantly high in leaves and very low in tubers, and the highest expression was observed in mature leaves in summer. In contrast to the DzDXR transcription, diosgenin was present predominantly in tubers and in minute quantities in leaves. Because diosgenin is very likely formed mainly in the cytosol of mature leaf cells of Dioscorea and the plastidic IPP and DMAPP produced by the MEP pathway can be transported into the cytosol, the consistently high expression of DzDXR detected in mature leaf of D. zingiberensis implies that the MEP pathway might play a significant role in diosgenin biosynthesis.

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Cloning and expression profile of 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene from an oil-bearing rose

Cited by 2 publications

References 19 publications

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

Cloning, characterization, and expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene from Dioscorea zingiberensis

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