Cloning and Functional Analysis of NoMYB60 Gene Involved in Flavonoid Biosynthesis in Watercress (Nasturtium officinale R. Br.)

Abstract

The MYB60 gene belongs to the R2R3-MYB subfamily, which includes the MYB31/30/96/94 genes. Although these genes have been shown to respond to heat and drought stresses, their role in flavonoid synthesis remains unclear. In this study, NoMYB60 was cloned from watercress and its structure and function were analyzed. Sequence structure analysis showed that NoMYB60 had a highly conserved R2R3 DNA-binding region at the N-terminus. Under the treatment of ABA, SA or MeJA, the expression level of NoMYB60 first significantly increased and then decreased, indicating that ABA, SA and MeJA positively regulated NoMYB60. The subcellular localization of NoMYB60-GFP indicated that NoMYB60 was localized in the nuclear region, which is consistent with the molecular characterization of the transcription factor. Gene silencing experiments were also performed to further test the function of NoMYB60. The result showed that virus-induced silencing of NoMYB60 affected the expression of enzyme genes in flavonoid synthesis pathways and promoted the synthesis of flavonoids. Moreover, we discovered that NoMYB60 interacts with NoBEH1/2. In this study, provides a reference for research on the regulation mechanism of flavonoid synthesis in Cruciferae and other crops.

Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato

“…Dai et al [ 36 ] reported that the cotton GhMYB6 gene had only one highly conserved SANT domain, indicating that it might belong to a different subfamily. SlMYB86-like was predicted to be localized to the nucleus, which was consistent with the results of Luo et al [ 37 ] and Ma et al [ 38 ], as well as with the structural characteristics of TFs. Analysis of the SlMYB86-like protein revealed that it is hydrophilic, which is consistent with the results of Hu et al [ 39 ].…”

Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana

“…At the same time, 'Beta' is also used as a processing variety for brewing and making juices [20]. MYB TFs are widely present in animals, plants, and fungi and are also one of the largest TF families in plants, possessing multiple biological functions [21]. It is not only involved in the regulation of plant growth and development [22,23], but it also participates in plant tolerance to stresses such as drought, salinity, and cold [24][25][26].…”

“…In order to obtain homozygous T 3 -generation Arabidopsis, the lines with relatively high expression were further cultured. Subsequently, salinity and drought treatments were carried out using T 3 -generation Arabidopsis [21].…”