Cloning and Functional Analysis of <i>CncC</i> and <i>Keap1</i> Genes in Silkworm

Hu, Jingsheng; Chen, Jian; Wang, Hui; Mao, Tingting; Li, Jinxin; Cheng, Xiaoyu; Hu, Jiahuan; Xue, Bin; Li, Bing

doi:10.1021/acs.jafc.7b05820

Cited by 33 publications

(14 citation statements)

References 37 publications

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“…In this study, the larva epidermis became transparent and showed the characteristics of mature larva after high temperature induction (Fig 1A), the survival and pupation rate of silkworm were significantly decreased (Fig 1D), which was in consistent with previous studies [10]. BmCncC/keap1 pathway plays an important role in response to oxidative stress and regulation of antioxidative and detoxification genes expression [22]. Whether this pathway involved in metamorphosis processes and regulates ecdysone-synthesis P450 genes in silkworm have not been reported.…”

Section: Discussionsupporting

confidence: 90%

BmCncC/keap1-pathway is involved in high-temperature induced metamorphosis regulation of silkworm,Bombyx mori

Mao

Zhang

et al. 2018

Preprint

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The global warming has affected the growth, development and reproduction of insects. However, the molecular mechanism of high temperature stress-mediated metamorphosis regulation of lepidopteran insect has not been elucidated. In this study, the relationship between the insect developmental process and endogenous hormone level was investigated under high temperature (36 ° C) stress in Bombyx mori (B. mori). The results showed that the duration of 5th instar larvae were shortened by 28 ± 2 h, and the content of 20E was up-regulated significantly after 72 h of high temperature treatment, while the transcription levels of 20E response genes E93, Br-C, USP, E75 were up-regulated 1.35, 1.25, 1.28, and 1.27-fold, respectively. The high temperature treatment promoted the phosphorylation level of Akt and the downstream BmCncC/keap1 pathway was activated, the transcription levels of 20E synthesis-related genes cyp302a1, cyp306a1, cyp314a1 and cyp315a1 were up-regulated by 1.12, 1.51, 2.17 and 1.23-fold, respectively. After treatment with double stranded RNA of BmCncC (dsBmCncC) in BmN cells, the transcription levels of cyp302a1 and cyp306a1 were significantly decreased, whereas up-regulated by 2.15 and 1.31-fold, respectively, after treatment with CncC activator Curcumin. These results suggested that BmCncC/keap1-mediated P450 genes (cyp302a1, cyp306a1) expression resulted in the changes of endogenous hormone level, which played an important role in the regulation of metamorphosis under high temperature stress. Studies provide novel clues for understanding the CncC/keap1 pathway-mediated metamorphosis regulation mechanism in insects.Author SummaryMammalian nuclear transcription factor Nrf2 (NF-E2-related factor 2) plays an important role in the stress response of cells. CncC is a homolog of mammalian Nrf2 in insect, regulating the genes expression of insect antioxidant enzymes and cytochrome P450 detoxification enzyme. Evidence suggests that the CncC/Keap1 pathway also plays an important role in regulating insect development. Here, we investigated the regulatory mechanism between the CncC/Keap1 pathway and metabolism of silkworm hormones in Lepidoptera. We found that high temperature induction accelerated the development of silkworm, the ecdysone content and related metabolic genes in hemolymph were significantly up-regulated, the CncC/Keap1 pathway was activated, and the expression of BmCncC was significantly increased, indicating that the Cncc/Keap1 pathway plays an important role in this process. The expression of cyp302a1 and cyp306a1 was significantly decreased by RNA interference with BmCncC, which indicated that CncC in silkworm had a regulatory relationship with downstream 20E synthetic gene. In summary, the results indicate that the CncC/Keap1 pathway plays an important role in regulating hormone metabolism in silkworm, providing a basis for further study of the relationship between CncC/Keap1 pathway and development in insects.

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Section: Discussionsupporting

confidence: 90%

BmCncC/keap1-pathway is involved in high-temperature induced metamorphosis regulation of silkworm,Bombyx mori

Mao

Zhang

et al. 2018

Preprint

Self Cite

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“…Subsequently, upregulation of different detoxification enzymes, especially P450s, by different chemicals were detected in other insects such as the fruit fly (Amichot et al, 1998), the black swallowtail caterpillar (Papilio polyxenes) (Berenbaum et al, 1990), the tobacco hornworm (Snyder et al, 1993), the mosquito Anopheles (Epis et al, 2014;Vontas et al, 2005), the tobacco cutworm (Spodoptera litura) (B. , and the silk moth (Bombyx mori) (J. Hu et al, 2018). In this century, using new transcriptomic strategies such as Micro-array (King-Jones et al, 2006;Le Goff et al, 2006;Lin et al, 2010;Vontas et al, 2005) and RNAseq (Coelho et al, 2015;Simma et al, 2019;Wilding, 2018), a much more complex diversification of insect xenobiotic response systems has been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Xenobiotic responses in insects

Gao

Qiao

Peng

et al. 2022

Arch Insect Biochem Physiol

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Insects have evolved a powerful detoxification system to protect themselves against environmental and anthropogenic xenobiotics including pesticides and nanoparticles.The resulting tolerance to insecticides is an immense problem in agriculture. In this study, we summarize advances in our understanding of insect xenobiotic responses: the detoxification strategies and the regulation mechanisms against xenobiotics including nanoparticles, the problem of response specificity and the potential usefulness of this study field for an elaborate pest management. In particular, we highlight that versatility of the detoxification system relies on the relatively unspecific recognition of a broad range of potential toxic substances that trigger either of various canonical xenobiotic responses signaling pathways, including CncC/Keap1, HR96, AHR/ARNT, GPCR, and MAPK/CREB. However, it has emerged that the actual response to an inducer may nevertheless be specific. There are two nonexclusive possibilities that may explain response specificity: (1) differential cross-talk between the known pathways and (2) additional, yet unidentified regulators and pathways of detoxification. Hence, a deeper and broader understanding of the regulation mechanisms of xenobiotic response in insects in the future might facilitate the development and application of highly efficient and environmentally friendly pest control methods, allowing us to face the challenge of the world population growth.

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“…The domesticated silkworm (Bombyx mori), is a silk insect with enormous economic value for the sericulture industry. China produces more than 78% of raw silk worldwide (Hu et al, 2018). The silk gland synthesizes and secretes silk fibre, which has been widely used in the silk products for thousands of years (Xia et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Enhanced silk yield in transgenic silkworm (Bombyx mori) via ectopic expression of BmGT1‐L in the posterior silk gland

Tang

Liu

Shi

et al. 2020

Insect Molecular Biology

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The silkworm is an economically important insect producing plentiful silk fibre in the silk gland. In this study, we reported a cross‐talk between the fat body, silk gland and midgut through a glycine‐serine biosynthetic pathway in the silkworm. Amino acid sequence and functional domains of glycine transporter gene BmGT1‐L were mapped. Our results indicated that BmGT1‐L was specifically expressed in the midgut microvilli and persistently expressed during the feeding stages. RNA interference of BmGT1‐L activated glycine biosynthesis, and BmGT1‐L overexpression facilitated serine biosynthesis in the BmN4‐SID1 cell. In addition, silkworms after FibH gene knock‐out or silk gland extirpation showed markedly decreased BmGT1‐L transcripts in the midgut and disturbed glycine‐serine biosynthesis as silk yield decreased. Finally, BmGT1‐L ectopic expression in the posterior silk gland promoted glycine biosynthesis, and enhanced silk yield via increasing fibroin synthesis. These results suggested that cross‐talk between tissues can be used for enhancing silk yield in the silkworm.

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Cloning and Functional Analysis of CncC and Keap1 Genes in Silkworm

Cited by 33 publications

References 37 publications

BmCncC/keap1-pathway is involved in high-temperature induced metamorphosis regulation of silkworm,Bombyx mori

BmCncC/keap1-pathway is involved in high-temperature induced metamorphosis regulation of silkworm,Bombyx mori

Xenobiotic responses in insects

Enhanced silk yield in transgenic silkworm (Bombyx mori) via ectopic expression of BmGT1‐L in the posterior silk gland

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