Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)

Wu, Jiandong; Jiang, Chengxi; Hong, Zhu; Jiang, Haiyang; Cheng, Beijiu; Zhu, Suwen

doi:10.4238/2015.may.22.17

Cited by 9 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Examples are the amylose extender1 ( ae1 ) and s hrunken2 ( sh2 ), known also for their significant association with starch pasting properties [14]; brittle endosperm2 ( bt2 ); shrunken1 ( sh1 ); and dull endosperm 1 ( du1 ), known for a significant association with kernel compositional traits. The latter, dull endosperm 1 gene (GRMZM2G141399, du1 ), encodes a starch synthase and is a determinant of the structure of endosperm starch in maize [28, 29]. This gene was found on chromosome 10 at a distance of approximately 46 kb and 564 kb downstream of two identified associated genomic regions with breakdown viscosity, one of the flour pasting properties studied in this work, and therefore not within the confidence intervals (59,574–60,031 kb and 60,092–60,351 kb, respectively).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour

et al. 2019

View full text Add to dashboard Cite

Background Maize is a crop in high demand for food purposes and consumers worldwide are increasingly concerned with food quality. However, breeding for improved quality is a complex task and therefore developing tools to select for better quality products is of great importance. Kernel composition, flour pasting behavior, and flour particle size have been previously identified as crucial for maize-based food quality. In this work we carried out a genome-wide association study to identify genomic regions controlling compositional and pasting properties of maize wholemeal flour. Results A collection of 132 diverse inbred lines, with a considerable representation of the food used Portuguese unique germplasm, was trialed during two seasons, and harvested samples characterized for main compositional traits, flour pasting parameters and mean particle size. The collection was genotyped with the MaizeSNP50 array. SNP-trait associations were tested using a mixed linear model accounting for genetic relatedness. Fifty-seven genomic regions were identified, associated with the 11 different quality-related traits evaluated. Regions controlling multiple traits were detected and potential candidate genes identified. As an example, for two viscosity parameters that reflect the capacity of the starch to absorb water and swell, the strongest common associated region was located near the dull endosperm 1 gene that encodes a starch synthase and is determinant on the starch endosperm structure in maize. Conclusions This study allowed for identifying relevant regions on the maize genome affecting maize kernel composition and flour pasting behavior, candidate genes for the majority of the quality-associated genomic regions, or the most promising target regions to develop molecular tools to increase efficacy and efficiency of quality traits selection (such as “breadability”) within maize breeding programs. Electronic supplementary material The online version of this article (10.1186/s12870-019-1729-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Suspensions of agrobacterium strain EHA105 cells carrying the effector plasmid were mixed in equal volumes with EHA105 cells carrying the reporter plasmids and infiltrated into N. benthamiana leaves. The Agrobacterium -mediated transient expression assays, GUS staining, and enzyme activity assays were performed as previously described (Yan et al, 2014; Wu et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

The DOF-Domain Transcription Factor ZmDOF36 Positively Regulates Starch Synthesis in Transgenic Maize

Chen

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

Starch synthesis is a complex process that influences crop yield and grain quality in maize. Many key enzymes have been identified in starch biosynthesis; however, the regulatory mechanisms have not been fully elucidated. In this study, we identified a DOF family gene, ZmDOF36 , through transcriptome sequencing analysis. Real-time PCR indicated that ZmDOF36 was highly expressed in maize endosperm, with lower expression in leaves and tassels. ZmDOF36 is a typical DOF transcription factor (TF) that is localized to the nucleus and possesses transcriptional activation activity, and its transactivation domain is located in the C-terminus (amino acids 227–351). Overexpression of ZmDOF36 can increase starch content and decrease the contents of soluble sugars and reducing sugars. In addition, abnormal starch structure in transgenic maize was also observed by scanning electron microscopy (SEM). Furthermore, the expression levels of starch synthesis-related genes were up-regulated in ZmDOF36 -expressing transgenic maize. ZmDOF36 was also shown to bind directly to the promoters of six starch biosynthesis genes, ZmAGPS1a , ZmAGPL1 , ZmGBSSI , ZmSSIIa , ZmISA1 , and ZmISA3 in yeast one-hybrid assays. Transient expression assays showed that ZmDOF36 can activate the expression of ZmGBSSI and ZmISA1 in tobacco leaves. Collectively, the results presented here suggest that ZmDOF36 acts as an important regulatory factor in starch synthesis, and could be helpful in devising strategies for modulating starch production in maize endosperm.

show abstract

“…The core regulatory element like TATA box and CAAT box were identified in all the taxa under study except rice. The presence of TATA box, CAAT box and G box in maize was also reported [ 32 ]…”

Section: Discussionmentioning

confidence: 93%

Conservation and divergence of Starch Synthase III genes of monocots and dicots

Mishra¹,

Kumar²,

Mohan³

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Starch Synthase (SS) plays an important role in extending the α-1,4 glucan chains during starch biosynthesis by catalyzing the transfer of the glucosyl moiety from ADP-glucose to the non-reducing end of a pre-existing glucan chain. SS has five distinct isoforms of which SSIII is involved in the formation of longer glucan chain length. Here we report identification and detailed characterization of ‘true’ orthologs of the well-characterized maize SSIII (ZmSSIII), among six monocots and two dicot species. ZmSSIII orthologs have nucleotide sequence similarity ranging from 56–81%. Variation in gene size among various orthologs ranged from 5.49 kb in Arabidopsis to 11.62 kb in Brachypodium and the variation was mainly due to intron size and indels present in the exons 1 and 3. Number of exons and introns were highly conserved among all orthologs however. While the intron number was conserved, intron phase showed variation at group, genera and species level except for intron 1 and 5. Several species, genera, and class specific cis-acting regulatory elements were identified in the promoter region. The predicted protein size of the SSIII orthologs ranged from 1094 amino acid (aa) in Arabidopsis to 1688 aa in Brachypodium with sequence identity ranging from 60%-89%. The N-terminal region of the protein was highly variable whereas the C-terminal region containing the Glycosyltransferase domain was conserved with >80% sequence similarity among the orthologs. In addition to confirming the known motifs, eleven novel motifs possibly providing species, genera and group specific functions, were identified in the three carbohydrate binding domains. Despite of significant sequence variation among orthologs, most of the motifs and their relative distances are highly conserved among the orthologs. The 3-D structure of catalytic region of SSIII orthologs superimposed with higher confidence confirming the presence of similar binding sites with five unidentified conserved regions in the catalytic (glycosyltransferase) domain including the pockets involved in catalysis and binding of ligands. Homeologs of wheat SSIII gene showed tissue and developmental stage specific expression pattern with the highest expression recorded in developing grains.

show abstract

Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)

Cited by 9 publications

References 31 publications

Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour

Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour

The DOF-Domain Transcription Factor ZmDOF36 Positively Regulates Starch Synthesis in Transgenic Maize

Conservation and divergence of Starch Synthase III genes of monocots and dicots

Contact Info

Product

Resources

About