Cloning and Functional Characterization of a Flavonoid Transport-Related MATE Gene in Asiatic Hybrid Lilies (Lilium spp.)

Abstract

Previous studies have suggested that multidrug and toxic compound extrusion (MATE) proteins might be involved in flavonoid transportation. However, whether MATE proteins are involved in anthocyanin accumulation in Lilium is unclear. Here, a flavonoid transport-related MATE candidate gene, LhDTX35, was cloned from the Asiatic hybrid lily cultivar ‘Tiny Padhye’ by rapid amplification of 5’ and 3’ cDNA ends (RACE) and found to encode 507 amino acids. BLASTx results indicated that LhDTX35 showed high homology to the DTX35 genes of other species. Bioinformatics analysis predicted that the protein encoded by LhDTX35 possessed 12 typical transmembrane segments and had functional domains typical of the MATE-like superfamily. Phylogenetic analysis grouped LhDTX35 in the same clade as the DTX35 of other species. Notably, the expression pattern of LhDTX35 was positively correlated with floral anthocyanin accumulation in ‘Tiny Padhye’. A subcellular localization assay showed that the protein encoded by LhDTX35 was plasmalemma localized but not nuclear, indicating that the LhDTX35 gene may function as a carrier protein to transport anthocyanins in Lilium. Functional complementation of the Arabidopsis DTX35 gene demonstrated that LhDTX35 could restore silique-infertility and the anthocyaninless phenotype of an Arabidopsis DTX35 mutant. These results indicated that LhDTX35 might be involved in anthocyanin accumulation in Lilium.

Multifaceted roles of a light-responsive factor LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Liliumspp.)

“…GST, acting as a molecules carrier, could mobilizes and transported anthocyanins from the cytoplasm to the tonoplast in the process of synthetic anthocyanin being transport and storage in vacuoles (Sun et al, 2012). Xu et al (2020) verified that the MATE family member DTX35 is related to lily flavonoid transport. Cao et al(2021b)revealed the anthocyanin transport mechanism mediated by GST in lily.…”

Metabolome and Transcriptome Reveal Chlorophyll, Carotenoid, and Anthocyanin Jointly Regulate the Color Formation of Triadica sebifera

“…The findings of previous studies have indicated that anthocyanin transport proteins may play roles in plant vacuoles (Han et al 2022, Pal et al 2023), and PpGST1 , MdGSTF6 , and LcGST4 have been identified as genes encoding proteins associated with the transport of anthocyanins (Hu et al 2016, Jiang et al 2019, Zhao et al 2020). Moreover, LhDTX35 (encoding a MATE) has been established to play a key role in regulating the accumulation of anthocyanins (Xu et al 2020). In the present study, we detected eight GST genes and eight MATE genes characterized by high expression in purple leaves, thereby indicating these genes may be responsible for the transport of anthocyanins during the development of purple leaf coloration.…”

“…Moreover, LhDTX35 (encoding a MATE) has been established to play a key role in regulating the accumulation of anthocyanins (Xu et al 2020). In the present study, we detected eight GST genes and eight MATE genes characterized by high expression in purple leaves, thereby indicating these genes may be responsible for the transport F I G U R E 1 1 Heatmap of DEGs in the carotenoid synthesis pathway.…”

Integration of Metabolome and Transcriptome Reveals the Relationship of Benzenoid–Phenylpropanoid Pigment and Aroma in Purple Tea Flowers

“…is half purple (lower part) and half white (upper part), which is similar to BT flowers. An MATE gene LhDTX35 might function as a carrier protein to transport anthocyanins ( Xu et al, 2020 ). Furthermore, a flavonoid carrier similar to mammalian bilitranslocase (BTL) was identified in grape, and it may participate in long distance transport of flavonoids for its presence in vascular bundles ( Petrussa et al, 2013 ).…”