Cloning and Functional Characterization of Chalcone Isomerase Genes Involved in Anthocyanin Biosynthesis in &lt;i&gt;Clivia miniata&lt;/i&gt;

Liu, Yue; Xue, Xinxin; Zhao, Chunli; Zhang, Jia; Li, Meng; Li, Xiangyu; Li, Yueqing; Gao, Xiang

doi:10.48130/opr-2021-0002

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…Detailed methods for the prokaryotic expression of recombinant proteins and enzymatic assay have been introduced in earlier studies [ 76 , 77 ]. Briefly, the pET32a vector containing GmOCS was transformed into E. coli BL21 (DE3) and inducted by IPTG (isopropyl-β-D-thiogalactopyranoside).…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Han

Shao

Gao

et al. 2023

IJMS

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Soybean is a worldwide crop that offers valuable proteins, fatty acids, and phytonutrients to humans but is always damaged by insect pests or pathogens. Plants have captured sophisticated defense mechanisms in resisting the attack of insects and pathogens. How to protect soybean in an environment- or human-friendly way or how to develop plant-based pest control is a hotpot. Herbivore-induced plant volatiles that are released by multiple plant species have been assessed in multi-systems against various insects, of which (E)-β-ocimene has been reported to show anti-insect function in a variety of plants, including soybean. However, the responsible gene in soybean is unknown, and its mechanism of synthesis and anti-insect properties lacks comprehensive assessment. In this study, (E)-β-ocimene was confirmed to be induced by Spodoptera litura treatment. A plastidic localized monoterpene synthase gene, designated as GmOCS, was identified to be responsible for the biosynthesis of (E)-β-ocimene through genome-wide gene family screening and in vitro and in vivo assays. Results from transgenic soybean and tobacco confirmed that (E)-β-ocimene catalyzed by GmOCS had pivotal roles in repelling a S. litura attack. This study advances the understanding of (E)-β-ocimene synthesis and its function in crops, as well as provides a good candidate for further anti-insect soybean improvement.

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Section: Methodsmentioning

confidence: 99%

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Han

Shao

Gao

et al. 2023

IJMS

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“…To examine the floral volatilized components, budding, halfopen, and fully open flowering phases of Chinese herbaceous peony species' cultivars were enclosed in a transparent gadget, specifically made of inorganic materials, and sampled. To determine the expression levels of genes, triplicate samples from the three flowering phases were collected for RNA extraction as described elsewhere [70].…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Integration of RNA-Seq and Metabolite Analysis Reveals the Key Floral Scent Biosynthetic Genes in Herbaceous Peony

Kimani,

Wang,

Xie

et al. 2024

Horticulturae

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Floral scent is an essential and genetically complex trait in herbaceous peonies (Paeonia lactiflora Pall.); however, specific genes related to metabolic and regulatory networks remain scantily studied. Our study integrated metabolite profiling and RNA-sequencing to screen floral scent biosynthetic genes. Hence, the major molecules identified by headspace collection combined with cultivar-specific GC-MS analysis were geraniol, β-caryophyllene, 2-phenylethanol (2-PE), citronellol, and 1,8-cineole. Genes related to terpenoids and 2-PE biosynthesis were identified after the assembly and annotation of the P. lactiflora transcriptomes. Eight angiosperm-specific terpene synthases (TPSs) from the TPS-a and TPS-b clades, as well as enzymes linked to 2-PE synthesis such as aromatic amino acid decarboxylase (AADC), phenylacetaldehyde reductase (PAR), and geranial reductase (GER) were identified. The biochemical analysis of the enzymes encoded by PlPAR1 and PlGER1 generated 2-PE from phenylacetaldehyde (PAld). The pairwise alignment of AADC1 reveals a splice variant lacking a 124 bp fragment, thus highlighting the possible role of alternative splicing in modulating floral scent composition. This study offers insights into the molecular-level biosynthesis of terpenoids and 2-PE in Peonia taxa, and provides the basis for the functional characterization, breeding, and bioengineering of prospective candidate genes for the production of floral volatiles in the Paeonia genus.

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“…Furthermore, the CHI gene in G. biloba L. was transformed into E. coli, and the activity of CHI showed a strong correlation with the flavonoid content [11]. Additionally, novel CmCHI genes from Clivia miniata were discovered, which were shown to be strongly expressed in anthocyanin-accumulating tissues [12]. Moreover, CHI co-expression may also have a profound influence on the deposition of flavonoids [13].…”

Section: Introductionmentioning

confidence: 99%

Cloning, Identification, and Functional Analysis of the Chalcone Isomerase Gene from Astragalus sinicus

Zhang

et al. 2023

Genes

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Astragalus sinicus is an important winter-growing cover crop. It is widely utilized, not only as a cover crop for its benefits in fertilizing the soil but also as a landscape ground cover plant. Anthocyanins are involved in the pigmentation of plants in leaves and flowers, which is a crucial characteristic trait for A. sinicus. The formation of anthocyanins depends significantly on the enzyme chalcone isomerase (CHI). However, research on the CHI gene of A. sinicus remains unexplored. The rapid amplification of cDNA ends (RACE) approach was used in this research to clone the CHI sequence from A. sinicus (AsiCHI). The expression profiles of the AsiCHI gene in multiple tissues of A. sinicus were subsequently examined by qRT-PCR (Quantitative Real-Time PCR). Furthermore, the function of the AsiCHI was identified by the performance of ectopic expression in Arabidopsis (Arabidopsis thaliana). The outcomes revealed that the full-length cDNA of the AsiCHI gene (GeneBank: OQ870547) measured 972 bp in length and included an open reading frame of 660 bp. The encoded protein contains 219 amino acids with a molecular weight of 24.14 kDa and a theoretical isoelectric point of 5.11. In addition, the remarkable similarity between the AsiCHI protein and the CHI proteins of other Astragalus species was demonstrated by the sequence alignment and phylogenetic analysis. Moreover, the highest expression level of AsiCHI was observed in leaves and showed a positive correlation with anthocyanin content. The functional analysis further revealed that the overexpression of AsiCHI enhanced the anthocyanidin accumulation in the transgenic lines. This study provided a better understanding of AsiCHI and elucidated its role in anthocyanin production.

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Cloning and Functional Characterization of Chalcone Isomerase Genes Involved in Anthocyanin Biosynthesis in <i>Clivia miniata</i>

Cited by 5 publications

References 57 publications

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Integration of RNA-Seq and Metabolite Analysis Reveals the Key Floral Scent Biosynthetic Genes in Herbaceous Peony

Cloning, Identification, and Functional Analysis of the Chalcone Isomerase Gene from Astragalus sinicus

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