Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate

Özavci, Hamza; Kaplan, Mustafa

doi:10.3906/sag-1808-98

Cited by 3 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Turkey is an endemic country for CL, with the main causative agent as L. tropica . 32 Although about a quarter of our VL cases, the cause was L. tropica , cutaneous lesions were not found in any of them. Concomitant cutaneous lesions should be investigated, and each patient with cutaneous lesions should be examined in detail regarding visceral spread.…”

Section: Discussionmentioning

confidence: 70%

Identi̇fi̇cati̇on of Lei̇shmani̇a Tropi̇ca From Pedi̇atri̇c Vi̇sceral Lei̇shmani̇asi̇s i̇n Southern Mediterranean Region of Turkey.

Alabaz

Eroglu²,

Elçi³

et al. 2022

Mediterr J Hematol Infect Dis

View full text Add to dashboard Cite

Background; Protozoa of the genus Leishmania are obligate intracellular parasites and Leishmania species cause a spectrum of species-specific clinical symptoms known as cutaneous, mucocutaneous and visceral leishmaniasis. Leishmania major and Leishmania tropica are known to cause cutaneous leishmaniasis, while Leishmania infantum and Leishmania donovani cause visceral leishmaniasis (VL). However, molecular studies in recent years have shown that Leishmania species cause different clinical symptoms. Objectives; Our aim is to evaluate the relationship between clinical and molecular characterization of leishmania isolates in children with VL defined in Turkey, which is an intercontinental transitional region. Methods; The clinical diagnosis of VL was confirmed by the detection of amastigotes in the bone marrow aspirate and/or the rK39 test and/or molecular methods (genus specific PCR, Real-Time PCR, ITS1 PCR-RFLP, DNA sequencing). Results: Most of the VL patients were referred from the districts of Adana (53.3%) and others from neighboring provinces; Hatay (16.6%), Osmaniye (3%), Gaziantep (3%), Adıyaman (3%) and 20% case were Syrian emigrants. A total of 30 patients with a clinical diagnosis of VL were confirmed by different diagnostic methods. It was found that 93% positive with microscopic examination, 79.1% with rK39 dipstick test, and 60% with genus specific PCR assay in clinical samples. The Leishmania isolates were identified as L. infantum (40%), L. donovani (26.7%) and L. tropica (23.3%) using Real Time PCR, ITS1 PCR-RFLP and DNA sequencing. There was no cutaneous finding in any case in clinical examination. The most common clinical findings were fever (93.3%), splenomegaly (90%), followed by hepatomegaly (76.6%). The most common laboratory finding was thrombocytopenia (86.6%) followed by anemia (70%). Hemophagocytic lympho-histiocytosis was detected in bone marrow aspiration in two of our patients. Since pentavalent antimony salts treatment initially failed in four patients, it was necessary to switch to Liposomal-Amphotericin B treatment and was successfully treated. Conclusions: The presence of L. tropica in VL patients, despite the absence of cutaneous findings in any of the cases, shows that this strain can cause VL, contrary to conventional knowledge. In the Adana province, where this study was carried out, L. infantum from CL cases in previous studies should be taken into account and visceral spread in CL cases and accompanying cutaneous lesions in VL cases should be investigated in detail. Key Words; Visceral leishmaniasis, DNA Sequencing, Leishmania infantum, Leishmania donovani, Leishmania tropica

show abstract

Section: Discussionmentioning

confidence: 70%

Identi̇fi̇cati̇on of Lei̇shmani̇a Tropi̇ca From Pedi̇atri̇c Vi̇sceral Lei̇shmani̇asi̇s i̇n Southern Mediterranean Region of Turkey.

Alabaz

Eroglu²,

Elçi³

et al. 2022

Mediterr J Hematol Infect Dis

View full text Add to dashboard Cite

show abstract

“…It is a 22.1 kDa protein that is expressed in both forms of the Leishmania (amastigote and promastigote) 6 . Thiol-specific antioxidant protein has been reported to be strongly antigenic for both mice and humans and to stimulatea T-helper1dependent immune response in the host body, particularly by strong stimulation of cellular immunity 9 . Since, TSA protein has main roles in the life of the Leishmania parasite and it is able to induce an immune response when used as a DNA vaccine or as recombinant proteins against infection with Leishmania species, we can say that TSA protein and its gene represent good candidates for a vaccine.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2022

IJPSR

View full text Add to dashboard Cite

Cutaneous leishmania is a common medical problem in Syria, which has become yet more widespread in most of the Syrian governorates after the current war. An effective vaccine is needed to prevent a large scale spread of leishmaniasis in the country. In this regard, protein vaccination has shown promising prospects of creating this muchneeded vaccine. Thiol Specific Antioxident (TSA) plays a fundamental role in the life of parasites. Its gene provides protective properties in the form of DNA vaccines against infections by many species of parasites and bacteria. So far, the presence of this gene in the genome of the Syrian strains of L. tropica has not been proven. This study aimed to investigate the presence of the gene in the Syrian strain of Leishmania tropica promastigotes, clone and compare with the TSA sequence in other Leishmaniaspp. We isolated genomic DNA from these parasites, designed primers for amplification of the gene encoding this protein, and standardized the PCR conditions.

show abstract

Tp-e Interval, Tp-e/QT and Tp-e/QTc Ratios in Female Patients with Small Heart Syndrome

İbişoğlu¹,

Boyraz²

2022

BMB

View full text Add to dashboard Cite

Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate

Cited by 3 publications

References 40 publications

Identi̇fi̇cati̇on of Lei̇shmani̇a Tropi̇ca From Pedi̇atri̇c Vi̇sceral Lei̇shmani̇asi̇s i̇n Southern Mediterranean Region of Turkey.

Identi̇fi̇cati̇on of Lei̇shmani̇a Tropi̇ca From Pedi̇atri̇c Vi̇sceral Lei̇shmani̇asi̇s i̇n Southern Mediterranean Region of Turkey.

Untitled

Tp-e Interval, Tp-e/QT and Tp-e/QTc Ratios in Female Patients with Small Heart Syndrome

Contact Info

Product

Resources

About