1995
DOI: 10.1111/j.1365-2958.1995.mmi_17010069.x
|View full text |Cite
|
Sign up to set email alerts
|

Cloning and molecular characterization of two genes encoding adhesion proteins involved in Trichomonas vaginalis cytoadherence

Abstract: Cytoadherence to the vaginal epithelium is a critical step in infection by the eukaryotic flagellate Trichomonas vaginalis. Four trichomonad surface proteins (AP65, AP51, AP33 and AP23) mediate cytoadherence. The cDNA encoding the AP65 adhesin was isolated from a phagemid cDNA expression library by screening with antiserum and monoclonal antibody (mAb) raised against the purified trichomonad AP65 protein. Two clones, F11.2 and F11.5, coded for immuno-crossreactive recombinant proteins that possessed functional… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

3
76
1

Year Published

1995
1995
2007
2007

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 75 publications
(80 citation statements)
references
References 42 publications
3
76
1
Order By: Relevance
“…2B). These findings are consistent with previous results describing the expression features of the ap65 gene family in other T. vaginalis isolates (8,12,15) and show that our experimental system is suitable for the study of iron-regulated expression of the ap65-1 gene.…”
Section: Iron-induced Transcription In Trichomonas Vaginalissupporting
confidence: 82%
See 2 more Smart Citations
“…2B). These findings are consistent with previous results describing the expression features of the ap65 gene family in other T. vaginalis isolates (8,12,15) and show that our experimental system is suitable for the study of iron-regulated expression of the ap65-1 gene.…”
Section: Iron-induced Transcription In Trichomonas Vaginalissupporting
confidence: 82%
“…Iron, which is an essential nutrient for almost every organism, is particularly important for T. vaginalis as it regulates growth rate, metabolic activities, and the expression of certain virulence phenotypes such as cytoadherence and resistance to complement lysis (6 -11). At the molecular level, iron has been shown to up-regulate the expression of a number of cellular proteins including a group of putative adhesin molecules, the identities of which are still controversial (8,9,(12)(13)(14)(15)(16)(17)(18)(19). Iron has also been shown to regulate the phosphorylation level of a major surface immunogen P270, which may be responsible for immune evasion (20).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…Moreover, two of the four adhesins, AP65 and AP33, have been characterized, and their sequences are known (6,14,25). As can be inferred from their protein sequences, which have a striking homology with the hydrogenosomal enzymes malic dehydrogenase (decarboxylating) (2,6,15,22,25) and succinyl coenzyme A synthetase (14,23), respectively, these proteins could possess an intrinsic affinity for membranes. On the basis of these data, we can suppose that the use of solubilized proteins, instead of live intact parasites, as well as the use of trypsinized and glutaraldehyde-fixed epithelial cells, is not a more appropriate technique to detect adhesins.…”
mentioning
confidence: 99%
“…In the past, several reports have described the molecular characterization of the different steps involved in interaction of the parasite with the human vaginal epithelial cells. Adhesion of the parasite is thought to be mediated by four trichomonad surface proteins, reportedly AP65, AP51, AP33, and AP23 (5,6), which are believed to recognize specific proteins of the host by a ligand-receptor type of interaction (3,7,8), although the receptors in the host cell have not yet been identified. The putative role of these proteins in adhesion has been characterized by using HeLa cells, because of their epithelial nature and the genital epithelium origin.…”
mentioning
confidence: 99%