Cloning and molecular identification of triosephosphate isomerase gene from Apis cerana cerana and its role in response to various stresses

Zhou, Yi; Wang, Fang; Liu, Feng; Wang, Chen; Yan, Yan; Guo, Xingqi; Xu, Baohua

doi:10.1007/s13592-016-0436-8

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…After RNAi of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 , qRT-PCR was used to detect the mRNA levels of AccANT , AccCDK5r , AccCPR24 , AccERR , AccTP1 , AccCDK5 , AccCYP336A1 , AccSp10 , AccRBM11 , AccAK , AccMTNR1A , and AccAIF3 . These genes were proved to be involved in the stress response in our previous studies ( Chu et al, 2013 ; Liu X. et al, 2014 ; Wang et al, 2014 ; Chen et al, 2015 ; Li et al, 2016b , 2018a ; Zhang et al, 2016 ; Zhou et al, 2016 ; Zhu et al, 2016 ; Gao et al, 2017 ; Zhao et al, 2018 ).…”

Section: Methodsmentioning

confidence: 80%

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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DnaJ, also known as Hsp40, plays important roles in maintaining the normal physiological state of an organism under stress conditions by mediating essential processes, such as protein synthesis, degradation, folding and metabolism. However, the exact functions of most DnaJ members are not fully understood in insects. Here, we identified three genes, AccDnaJA1, AccDnaJB12, and AccDnaJC8, in Apis cerana cerana and explored their connection with the environmental stress response. Quantitative real-time PCR results showed that the mRNA levels of AccDnaJA1, AccDnaJB12, and AccDnaJC8 were all induced under cold, UV, H2O2 and different pesticides treatment. The expression patterns of AccDnaJB12 and AccDnaJC8 were upregulated by CdCl2 and HgCl2 stress, while the transcriptional levels of AccDnaJA1 were downregulated by CdCl2 and HgCl2 stress. Western blot findings further indicated that AccDnaJB12 protein levels were increased by some stress conditions. Knockdown of each of these three genes downregulated the transcriptional patterns of several stress response-related genes at different levels. Functional analysis further demonstrated that the resistance of A. cerana cerana to lambda-cyhalothrin stress was reduced with knockdown of AccDnaJA1, AccDnaJB12, or AccDnaJC8, indicating that these three genes may be involved in the tolerance to this pesticide. Taken together, these findings indicate that AccDnaJA1, AccDnaJB12, and AccDnaJC8 may play pivotal roles in the stress response by facilitating honeybee survival under some adverse circumstances. To our knowledge, this is the first report that reveals the roles of DnaJ family proteins under different adverse circumstances in A. cerana cerana.

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Section: Methodsmentioning

confidence: 80%

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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“…A 278-bp fragment of AccSp10 was obtained using primers SQ1 and SQ2 (listed in Table 1). The housekeeping gene β-actin from A. cerana cerana (XM640276) was used as a control gene, because it has been verified as the most stably expressed gene in honeybees (Scharlaken et al 2008;Zhou et al 2016). SYBR Premix Ex Taq (TaKaRa, Dalian, China) was used in a 25-μL volume on a CFX96™ Real-Time System (Bio-Rad, Hercules, CA, USA).…”

Section: Rt-qpcr Analysismentioning

confidence: 99%

The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses

Gao

Wang

Liu

et al. 2017

Cell Stress and Chaperones

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Serine proteinases play important roles in innate immunity and insect development. We isolated a serine proteinase gene, designated AccSp10, from the Chinese honeybees (Apis cerana cerana). RT-qPCR and a Western blot analysis at different pupal development stages indicated that AccSp10 might be involved in melanin formation in pupae and promote pupal development. In adult workers, the expression of AccSp10 was upregulated by treatments mimicking harmful environments such as the presence of Bacillus bombysepticus, different temperatures (4, 24 and 42 °C), HgCl, HO and paraquat; the exception was treatment with VC (vitamin C), which did not upregulate AccSp10 expression. Western blot confirmed the results. A disc diffusion assay indicated that recombinant AccSp10 accelerated E. coli cell death during stimulation with harmful substances (HgCl, paraquat and cumene hydroperoxide). These findings suggest that AccSp10 may be involved in the pupal development of Chinese honeybees and protection against microorganisms and abiotic harms.

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Characterization of the RACK1 gene of Aips cerana cerana and its role in adverse environmental stresses

Wang

Xie

Wang

et al. 2023

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Cloning and molecular identification of triosephosphate isomerase gene from Apis cerana cerana and its role in response to various stresses

Cited by 4 publications

References 37 publications

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses

Characterization of the RACK1 gene of Aips cerana cerana and its role in adverse environmental stresses

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