Cloning and nucleotide sequence analysis of theCandida albicansenolase gene

Abstract

The complete nucleotide sequence of the coding region as well as the flanking non-coding region of Candida albicans enolase gene was determined. A continuous open reading frame of 1323 nucleotides with no introns was identified. The deduced amino acid sequence showed 87% similarity to the enolases from the yeast Saccharomyces cerevisiae. The two isoforms of enolase are encoded by two non-tandemly arrayed genes in S. cerevisiae. However, DNA hybridisation analysis indicates that in C. albicans enolase is encoded by a single gene. The position of the transcription start site, putative TATA box and polyadenylation signal of the C. albicans enolase gene have been identified. The location of these sequences are similar to those of the S. cerevisiae enolase genes.

Purification of native enolase from medically important Candida species

“…The chromatography conditions were established on the assumption that the pI values of the enolases from the non‐ albicans Candida species would be similar to that of C. albicans . The enolase gene of C. albicans has been sequenced and the amino acid sequence deduced [22–24]. Using the deduced amino acid sequence, the theoretical pI value for the C. albicans enolase was calculated as 5.42 using the MacVector program (Oxford Molecular, Oxford, U.K.).…”