Cloning and Nucleotide Sequence of Thermostable Lipase Gene from<i>Pseudomonas fluorescens</i>SIK W1

Chung, Guk Hoon; Lee, Young Phil; Jeohn, Gwang Ho; Yoo, Ook Joon; Rhee, Jae-Sung

doi:10.1080/00021369.1991.10870954

Cited by 26 publications

(6 citation statements)

References 11 publications

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“…Studies were conducted on lipases from P. fluorescens strain B52, SIK W1, no. 33, LS107d2, HU380 (Chung et al , 1991; Johnson et al , 1992; Tan & Miller, 1992; Kumura et al , 1998; Kojima & Shimizu, 2003; Kojima et al , 2003; Jiang et al , 2005;), S. marcescens strains SM6, Sr41 (Akatsuka et al , 1994; Li et al , 1995;), Pseudomonas brassicacearum (Chabeaud et al , 2001) and Pseudomonas sp. strains MIS38 and KB700A (Amada et al , 2000; Rashid et al , 2001;).…”

Section: Classes Of Rtx Proteinsmentioning

confidence: 99%

RTX proteins: a highly diverse family secreted by a common mechanism

et al. 2010

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Repeats-in-toxin (RTX) exoproteins of Gram-negative bacteria form a steadily growing family of proteins with diverse biological functions. Their common feature is the unique mode of export across the bacterial envelope via the type I secretion system and the characteristic, typically nonapeptide, glycine- and aspartate-rich repeats binding Ca2+ ions. In this review, we summarize the current state of knowledge on the organization of rtx loci and on the biological and biochemical activities of therein encoded proteins. Applying several types of bioinformatic screens on the steadily growing set of sequenced bacterial genomes, over 1000 RTX family members were detected, with the biological functions of most of them remaining to be characterized. Activities of the so far characterized RTX family members are then discussed and classified according to functional categories, ranging from the historically first characterized pore-forming RTX leukotoxins, through the large multifunctional enzymatic toxins, bacteriocins, nodulation proteins, surface layer proteins, up to secreted hydrolytic enzymes exhibiting metalloprotease or lipase activities of industrial interest.

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Section: Classes Of Rtx Proteinsmentioning

confidence: 99%

RTX proteins: a highly diverse family secreted by a common mechanism

et al. 2010

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“…This assay procedure has been used widely to screen lipase‐positive clones [14–16,78, 80, 81]. However, different researchers have used different concentrations of Rhodamine B in the medium, such as 0.001% (w/v) [14–16,81] and 0.0002% (w/v) [73], which could be because lower concentrations of Rhodamine B do not always lead to a clear fluorescent halo. Another drawback of this protocol is that a clear zone is visible under UV light only when a high enzyme titre is obtained.…”

Section: Molecular Screening Of Lipasesmentioning

confidence: 99%

“…A conclusive lipase plate assay is the trioleoylglycerol/Rhodamine B agar plate assay described by Kouker and Jaeger [21]. This assay procedure has been used widely to screen lipase‐positive clones [14–16,78, 80, 81]. However, different researchers have used different concentrations of Rhodamine B in the medium, such as 0.001% (w/v) [14–16,81] and 0.0002% (w/v) [73], which could be because lower concentrations of Rhodamine B do not always lead to a clear fluorescent halo.…”

Section: Molecular Screening Of Lipasesmentioning

confidence: 99%

Lipase assays for conventional and molecular screening: an overview

Gupta

Rathi

Gupta

et al. 2003

Biotech and App Biochem

162

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Lipases are versatile biocatalysts that can perform innumerable different reactions. Their enantio-, chemo- and stereo-selective nature makes them an important tool in the area of organic synthesis. Unlike other hydrolases that work in aqueous phase, lipases are unique as they act at the oil/water interface. Besides being lipolytic, lipases also possess esterolytic activity and thus have a wide substrate range. Hence, the lipase assay protocols hold a significant position in the field of lipase research. Lipase activity can be estimated using a wide range of assay protocols that differ in terms of their basic principle, substrate selectivity, sensitivity and applicability. As the value of these enzymes continues to grow and new markets are exploited, development of new or improved enzymes will be a key element in the emerging realm of biotechnology. Hence, development of faster and simpler protocols incorporating newer and more specific substrates is the need of the hour. In this endeavour, methods that could be adopted for molecular screening occupy an important position. Here, an overview of the lipase assay protocols is presented with emphasis on the assays that can be adopted for the molecular screening of these biocatalysts.

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“…The size and organization of P. fluorescens lipase genes in genome have abundant diversity [26][27][28]. Typical lipase operon consists of lipase gene and the ABC secret components, aprD, aprE, and aprF [26].…”

Section: Discussionmentioning

confidence: 99%

“…Typical lipase operon consists of lipase gene and the ABC secret components, aprD, aprE, and aprF [26]. The genome organization analysis on the lipase operon of P. fluorescens no.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression of Pseudomonas fluorescens 26-2 Lipase Gene in Pichia pastoris and Characterizing for Transesterification

Yang

Zhang

Yan

2008

Appl Biochem Biotechnol

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Pseudomonas lipases are important biocatalysts widely used in a variety of industrial fields. An extracellular lipase gene lipA with 1,854-bp open reading frame was cloned from Pseudomonas fluorescens 26-2. The multialignment assay of the putative amino acid and the secondary structure prediction revealed this enzyme could be classified into the lipolytic subfamily I.3 and secreted via adenosine-triphosphate-binding cassette pathway. The lipA gene was integrated into Pichia pastoris GS115, and the methanol-inducible recombinants with Mut(S) and Mut(+) phenotypes were acquired. The characteristics and the transesterification capacity shown by this enzyme suggested it is a useful biocatalyst for biodiesel preparation.

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Cloning and Nucleotide Sequence of Thermostable Lipase Gene fromPseudomonas fluorescensSIK W1

Cited by 26 publications

References 11 publications

RTX proteins: a highly diverse family secreted by a common mechanism

RTX proteins: a highly diverse family secreted by a common mechanism

Lipase assays for conventional and molecular screening: an overview

Cloning and Expression of Pseudomonas fluorescens 26-2 Lipase Gene in Pichia pastoris and Characterizing for Transesterification

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