Abstract:The xynB gene of Bacillus subtilis subsp. spizizenii W23 is predicted to encode a xylan 1,4-beta-xylosidase. Application of XynB enzymes in industries is wide. Production of this enzyme in its host cells is naturally restricted by repression process. It will give certain beneficial to over-expressed the enzymes in other host-cells under inducing promoter. This study aimed to clone the xynB gene from Bacillus subtilis subsp. spizizenii W23, to pMMB67EH plasmid, and to over-express the xynB gene in Escherichia c… Show more
“…1. Other reports [42][43][44][45][46] are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariavum.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
“…1. Other reports [42][43][44][45][46] are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariavum.…”
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.
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