Cloning and overexpression of the ascorbate peroxidase gene from the yam (Dioscorea alata) enhances chilling and flood tolerance in transgenic Arabidopsis

Chen, Zhihua; Lu, Hong-Yang; Hua, Shumei; Lin, Kuan–Hung; Chen, Ningdan; Zhang, Yangwen; You, Ziyi; Kuo, Yun‐Wei; Chen, Shi-Peng

doi:10.1007/s10265-019-01136-4

Cited by 14 publications

(8 citation statements)

References 34 publications

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“…There have been few reports describing the molecular characterization of yam proteins in planta (Liu et al 2017 ; Chen et al 2019b ). Genetic transformation has been successful in D. rotundata and D. zingiberensis (Zhu et al 2009 ; Shi et al 2012 ; Nyaboga et al 2014 ) but the only transgenic yam line reported thus far is a D. zingiberensis mutant generated using the CRISPR/Cas9 system to reduce the expression of farnesyl pyrophosphate synthase mRNA, resulting in the depletion of squalene (Feng et al 2018 ).…”

Section: Current Research Status and Breeding Objectivesmentioning

confidence: 99%

An underutilized orphan tuber crop—Chinese yam : a review

Epping¹,

Laibach

2020

Planta

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Main conclusion The diversification of food crops can improve our diets and address the effects of climate change, and in this context the orphan crop Chinese yam shows significant potential as a functional food. Abstract As the effects of climate change become increasingly visible even in temperate regions, there is an urgent need to diversify our crops in order to address hunger and malnutrition. This has led to the re-evaluation of neglected species such as Chinese yam (Dioscorea polystachya Turcz.), which has been cultivated for centuries in East Asia as a food crop and as a widely-used ingredient in traditional Chinese medicine. The tubers are rich in nutrients, but also contain bioactive metabolites such as resistant starches, steroidal sapogenins (like diosgenin), the storage protein dioscorin, and mucilage polysaccharides. These health-promoting products can help to prevent cardiovascular disease, diabetes, and disorders of the gut microbiome. Whereas most edible yams are tropical species, Chinese yam could be cultivated widely in Europe and other temperate regions to take advantage of its nutritional and bioactive properties. However, this is a laborious process and agronomic knowledge is fragmented. The underground tubers contain most of the starch, but are vulnerable to breaking and thus difficult to harvest. Breeding to improve tuber shape is complex given the dioecious nature of the species, the mostly vegetative reproduction via bulbils, and the presence of more than 100 chromosomes. Protocols have yet to be established for in vitro cultivation and genetic transformation, which limits the scope of research. This article summarizes the sparse research landscape and evaluates the nutritional and medical applications of Chinese yam. By highlighting the potential of Chinese yam tubers, we aim to encourage the adoption of this orphan crop as a novel functional food.

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Section: Current Research Status and Breeding Objectivesmentioning

confidence: 99%

An underutilized orphan tuber crop—Chinese yam : a review

Epping¹,

Laibach

2020

Planta

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“…An Ascorbate peroxidase ( APX ) gene cloned from yam ( Dioscorea alata ) was shown to enhance tolerance to flood and chilling stresses in transgenic Arabidopsis (Rosa et al 2010 ; Bonifacio et al 2011 ; Chen et al 2019 ). A high-quality reference genome of mung bean enabled fast identification of synteny blocks associated with seed weight and nematode resistance through comparative analysis with soybean ( Glycine max ) and led to the development of functional markers for mung bean (Kang et al 2014 ).…”

Section: Development Of Genetic and Genomic Resources In Climate Resilient Orphan Cropsmentioning

confidence: 99%

Genetics and breeding for climate change in Orphan crops

et al. 2021

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Climate change is rapidly changing how we live, what we eat and produce, the crops we breed and the target traits. Previously underutilized orphan crops that are climate resilient are receiving much attention from the crops research community, as they are often the only crops left in the field after periods of extreme weather conditions. There are several orphan crops with incredible resilience to biotic and abiotic stresses. Some are nutritious, while others provide good sources of biofuel, medicine and other industrial raw materials. Despite these benefits, orphan crops are still lacking in important genetic and genomic resources that could be used to fast track their improvement and make their production profitable. Progress has been made in generating draft genomes of at least 28 orphan crops over the last decade, thanks to the reducing cost of sequencing. The implementation of a structured breeding program that takes advantage of additional modern crop improvement tools such as genomic selection, speed breeding, genome editing, high throughput phenotyping and breeding digitization would make rapid improvement of these orphan crops possible, but would require coordinated research investment. Other production challenges such as lack of adequate germplasm conservation, poor/non-existent seed systems and agricultural extension services, as well as poor marketing channels will also need to be improved if orphan crops were to be profitable. We review the importance of breeding orphan crops under the increasing effects of climate change, highlight existing gaps that need to be addressed and share some lessons to be learned from major crops.

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“…GSH-Px catalyzed the conversion of reduced glutathione (GSH) to oxidized glutathione (GSSG), which scavenged harmful free radicals, thus protecting the structure and function of cell membranes against damage by peroxides [48]. Upregulated APX expression could enhance the ability of plants to tolerate oxidation, which in turn enhanced their ability to resist stress [49]. Upregulated expression of LAR1 could enhance the salt tolerance and insect resistance of plants [48].…”

Section: Transcriptome Analysismentioning

confidence: 99%

Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana

Pan

Dong

et al. 2021

BMC Plant Biol

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Background As important forest tree species, biological stress and soil salinization are important factors that restrict the growth of Populus × euramericana. WRKYs are important transcription factors in plants that can regulate plant responses to biotic and abiotic stresses. In this study, PeWRKY31 was isolated from Populus × euramericana, and its bioinformation, salt resistance and insect resistance were analyzed. This study aims to provide guidance for producing salt-resistant and insect-resistant poplars. Results PeWRKY31 has a predicted open reading frame (ORF) of 1842 bp that encodes 613 amino acids. The predicted protein is the unstable, acidic, and hydrophilic protein with a molecular weight of 66.34 kDa, and it has numerous potential phosphorylation sites, chiefly on serines and threonines. PeWRKY31 is a zinc-finger C2H2 type-II WRKY TF that is closely related to WRKY TFs of Populus tomentosa, and localizes to the nucleus. A PeWRKY31 overexpression vector was constructed and transformed into Nicotiana tabacum L. Overexpression of PeWRKY31 improved the salt tolerance and insect resistance of the transgenic tobacco. Transcriptome sequencing and KEGG enrichment analysis showed the elevated expression of genes related to glutathione metabolism, plant hormone signal transduction, and MAPK signaling pathways, the functions of which were important in plant salt tolerance and insect resistance in the overexpressing tobacco line. Conclusions PeWRKY31 was isolated from Populus × euramericana. Overexpression of PeWRKY31 improved the resistance of transgenic plant to salt stress and pest stress. The study provides references for the generation of stress-resistant lines with potentially great economic benefit.

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Cloning and overexpression of the ascorbate peroxidase gene from the yam (Dioscorea alata) enhances chilling and flood tolerance in transgenic Arabidopsis

Cited by 14 publications

References 34 publications

An underutilized orphan tuber crop—Chinese yam : a review

An underutilized orphan tuber crop—Chinese yam : a review

Genetics and breeding for climate change in Orphan crops

Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana

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