Cloning and Promoter Identification of Acetoacetyl-CoA Thiolase Gene from Oil Palm, Elaeis guineensis Jacq.

Rahman, Khadijah Hanim Abdul; Samian, Mohammed Razip

doi:10.7763/ijesd.2014.v5.510

Cited by 2 publications

(1 citation statement)

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“…At the initial step of the MVA pathway, AACT catalyzes the biological Claisen condensation of two molecules of acetyl-CoA to form acetoacetyl-CoA, which is essential in producing terpenoid and isoprenoid compounds such as sterols, carotenoids and growth regulators. At present, the study of AACT genes in plants is less, what we do know is that AACT is found in Elaeis guineensis Jacq, Ganoderma lucidum and Bacopa monnieri (Rahman and Samian et al, 2014;Fang et al, 2013;Vishwakarma et al, 2012). However, the AACT gene has not been reported in C. nobile so far.…”

Section: Introductionmentioning

confidence: 83%

Cloning and Sequence Analysis of an Acetyl-CoA C-Acetyltransferase Gene (AACT) from Chamaemelum nobile

Fu¹,

Liu²,

Meng³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Section: Introductionmentioning

confidence: 83%

Cloning and Sequence Analysis of an Acetyl-CoA C-Acetyltransferase Gene (AACT) from Chamaemelum nobile

Fu¹,

Liu²,

Meng³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Molecular Cloning, Characterization, and Functional Analysis of Acetyl-CoA C-Acetyltransferase and Mevalonate Kinase Genes Involved in Terpene Trilactone Biosynthesis from Ginkgo biloba

et al. 2017

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Ginkgolides and bilobalide, collectively termed terpene trilactones (TTLs), are terpenoids that form the main active substance of Ginkgo biloba. Terpenoids in the mevalonate (MVA) biosynthetic pathway include acetyl-CoA C-acetyltransferase (AACT) and mevalonate kinase (MVK) as core enzymes. In this study, two full-length (cDNAs) encoding AACT (GbAACT, GenBank Accession No. KX904942) and MVK (GbMVK, GenBank Accession No. KX904944) were cloned from G. biloba. The deduced GbAACT and GbMVK proteins contain 404 and 396 amino acids with the corresponding open-reading frame (ORF) sizes of 1215 bp and 1194 bp, respectively. Tissue expression pattern analysis revealed that GbAACT was highly expressed in ginkgo fruits and leaves, and GbMVK was highly expressed in leaves and roots. The functional complementation of GbAACT in AACT-deficient Saccharomyces cerevisiae strain Δerg10 and GbMVK in MVK-deficient strain Δerg12 confirmed that GbAACT mediated the conversion of mevalonate acetyl-CoA to acetoacetyl-CoA and GbMVK mediated the conversion of mevalonate to mevalonate phosphate. This observation indicated that GbAACT and GbMVK are functional genes in the cytosolic mevalonate (MVA) biosynthesis pathway. After G. biloba seedlings were treated with methyl jasmonate and salicylic acid, the expression levels of GbAACT and GbMVK increased, and TTL production was enhanced. The cloning, characterization, expression and functional analysis of GbAACT and GbMVK will be helpful to understand more about the role of these two genes involved in TTL biosynthesis.

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Cloning and Promoter Identification of Acetoacetyl-CoA Thiolase Gene from Oil Palm, Elaeis guineensis Jacq.

Cited by 2 publications

References 26 publications

Cloning and Sequence Analysis of an Acetyl-CoA C-Acetyltransferase Gene (AACT) from Chamaemelum nobile

Cloning and Sequence Analysis of an Acetyl-CoA C-Acetyltransferase Gene (AACT) from Chamaemelum nobile

Molecular Cloning, Characterization, and Functional Analysis of Acetyl-CoA C-Acetyltransferase and Mevalonate Kinase Genes Involved in Terpene Trilactone Biosynthesis from Ginkgo biloba

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