Cloning and sequence analysis of an actin gene in aloe

Abstract

ABSTRACT. Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcriptionpolymerase chain reaction (RT-PCR). The result of sequence analysis for the amplified fragment revealed that the cloned actin gene was 1012 bp in length (GenBank accession No. KC751541.1) and contained a 924-bp coding region and encoded a protein consisting of 307 amino acids. Homologous alignment showed that it shared over 80 and 96% identity with the nucleotide and amino acid sequences of actin from other plants, respectively. In addition, the cloned gene was used for phylogenetic analyses based on the deduced amino acid sequences, and the results suggested that the actin gene is highly conserved in evolution. The findings of this study will be useful for investigating the expression patterns of other genes in Aloe.

Cloning the full-length CDNA of actin gene and analysing alliinase gene expression in tillering onion

“…In addition, their stable expression during the different developmental stages, under different conditions, and in different tissues implies that actin genes can be used as reference genes for normalization (Nakayama et al 2014;Liu et al 2015;Kim et al 2018;Wang et al 2021). The structure and expression of actin genes have been wellcharacterised in higher plants (Wen et al 2014). However, only a few actin genes from Allium species have been cloned and reported.…”