Cloning and Sequence Analysis of Wild Argali ISG15 cDNA

Abstract

The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by Ni2+ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA

“…Wild Argali’s weight is the highest among Caprinae, and the rams weigh up to 200 kg. Wild Argali are up to 1.27 meters tall at the shoulder, have more lean and less fat content, enabling fast growth, less fat, strong adaptability, high disease resistance as the main characteristics [ 2 ], and are an excellent genetic resource. It is important to exploit and make full use of the superior characteristics of Wild Argali in Xinjiang.…”