Cloning and sequencing of a cDNA encoding ascorbate peroxidase fromArabidopsis thaliana

Kubo, Akihiro; Saji, Hikaru; Tanaka, Kiyoshi; Kondo, Noriaki

doi:10.1007/bf00020011

Cited by 85 publications

(49 citation statements)

References 32 publications

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“…Among several clones identified as ripening-specific, one was found to be homologous to recently described sequences encoding cytosolic APX [15,16].…”

Section: Isolation Of a Pepper Cdna Encoding Apxmentioning

confidence: 89%

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Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum

et al. 1995

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The ascorbate peroxidase (APX) system was studied in Capsicum annuum during fruit ripening. A large increase in organelle APX activity was found during chloroplast-chromoplast transition whereas only a slight difference was detected in total fruit extracts. On native gels, four different isoforms were found in total fruit extracts but the patterns for red and green fruit were quite different. In isolated organelles, six isozymes were found and a comparison of the patterns showed significant differences. A cDNA encoding a cytosolic APX was cloned and sequenced. The corresponding transcript was shown to increase 3-4-fold during fruit ripening.

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“…Among several clones identified as ripening-specific, one was found to be homologous to recently described sequences encoding cytosolic APX [15,16].…”

Section: Isolation Of a Pepper Cdna Encoding Apxmentioning

confidence: 89%

“…2. Comparison of the derived bell pepper protein sequence with those from pea [15] and Arabidopsis [16]. Upper case letters in the consensus sequence indicate conservation across all three species.…”

Section: M-l Schantz Et Al/febs Letters 358 (1995) 149-152mentioning

confidence: 99%

Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum

et al. 1995

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“…Neither cloned and Arabidopsis [6,7]. SAP1 was also 30% identical over 250 cDNA had a transit peptide.…”

Section: E T a V R F C E P I K R R H P D I T Y A D L Y Q L 100mentioning

confidence: 98%

“…has been reported in pea [6], Arabidopsis [7], soybean [8], maize agarose gel, blotted on a nylon membrane filter, and hybridized to a [9], and spinach (Webb and Allen, accession number L20864).…”

Section: Northern Blot Analysismentioning

confidence: 99%

Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach

et al. 1995

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AsAP and monoclonal antibodies preparation will be described elsewas 1.6-fold higher with ascorbate than with guaiacol, which was where. Recombinant pBluescript SK-phagemids were rescued from similar to those of endogenous cytosolie ascorbate peroxidases, positive bacteriophage clones by in vivo excision by the procedure Here we conclude that SAP1 belongs to a new type of ascorbate provided by the manufacturer. The nucleotides of the isolated eDNA peroxidase from spinach, clone were sequenced by the dideoxy chain primer method modified for double stranded plasmid DNA.

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“…After phenol extraction and ethano1 precipitation, total RNA was used for RNA gel blot and slot blot hybridization experiments, cDNA synthesis, and polymerase chain reaction (PCR) analysis. We used the following cDNA and gene probes in these experiments: APX7 and APX2 from Arabidopsis encoding cytosolic isoforms of ascorbate peroxidase (Kubo et al, 1992(Kubo et al, ,1993Santos et al, 1996) andAPX3 encoding a putative membrane-associated cytosolic APX isoform (Arabidopsis Biological Resource Center; accession number VBVEBO9). Gene-specific probes were generated from these APX sequences by PCR amplification of the most variable coding regions.…”

Section: Rna and Dna Gel Blots And Rna Slot Blot Hybridizationmentioning

confidence: 99%

Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.

et al. 1997

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Exposure of Arabidopsis plants that were maintained under low light (200 pmol of photons m-2 sec-I) to excess light (2000 pmol of photons m-2 sec-I) for 1 hr caused reversible photoinhibition of photosynthesis. Measurements of photosynthetic parameters and the use of electron transport inhibitors indicated that a nove1 signal transduction pathway was initiated at plastoquinone and regulated, at least in part, by the redox status of the plastoquinone pool. This signal, which preceded the photooxidative burst of hydrogen peroxide (H202) associated with photoinhibition of photosynthesis, resulted in a rapid increase (within 15 min) in mRNA levels of two cytosolic ascorbate peroxidase genes ( A f X 1 and APX2). Treatment of leaves with exogenous reduced glutathione abolished this signal, suggesting that glutathione or the redox status of the glutathione pool has a regulatory impact on this signaling pathway. During recovety from photooxidative stress, transcripts for cytosolic glutathione reductase (GOR2) increased, emphasizing the role of glutathione in this stress.

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Cloning and sequencing of a cDNA encoding ascorbate peroxidase fromArabidopsis thaliana

Cited by 85 publications

References 32 publications

Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum

Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum

Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach

Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.

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