Cloning and Sequencing of an Ice Nucleation Active Gene of<i>Erwinia uredovora</i>

Michigami, Yasuyuki; Watabe, Shigeo; Abe, Keiko; Obata, Hitoshi; Arai, Soichi

doi:10.1271/bbb.58.762

Cited by 19 publications

(11 citation statements)

References 2 publications

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“…[27][28][29][30][31][32][33][34][35] Genomic DNA from P. antarctica IN-74 did not hybridize with the ice-nucleating inaZ gene from P. syringae 27) …”

Section: Discussionmentioning

confidence: 99%

Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleatingPseudomonas antarcticaIN-74

Muryoi

Kawahara

Obata

2003

Bioscience, Biotechnology, and Biochemistry

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Some ice-nucleating bacterial strains, including Pantoea ananatis (Erwinia uredovora), Pseudomonas fluorescens, and Pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. A novel ice-nucleating bacterium, Pseudomonas antarctica IN-74, was isolated from Ross Island, Antarctica. Cell-free ice nuclei from P. antarctica IN-74 were different from the conventional cell-free ice nuclei and showed a unique characterization. Cell-free ice nuclei were purified by centrifugation, filtration (0.45 microm), ultrafiltration, and gel filtration. In an ice-nucleating medium in 1 liter of cell culture, maximum growth was obtained with the production of 1.9 mg of cell-free ice nuclei. Ice nucleation activity in these cell-free ice nuclei preparations was extremely sensitive to pH. It was demonstrated that the components of cell-free ice nuclei were protein (33%), saccharide (12%), and lipid (55%), indicating that cell-free ice nuclei were lipoglycoproteins. Also, carbohydrate and lipid stains showed that cell-free ice nuclei contained both carbohydrate and lipid moieties.

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“…[27][28][29][30][31][32][33][34][35] Genomic DNA from P. antarctica IN-74 did not hybridize with the ice-nucleating inaZ gene from P. syringae 27) …”

Section: Discussionmentioning

confidence: 99%

Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleatingPseudomonas antarcticaIN-74

Muryoi

Kawahara

Obata

2003

Bioscience, Biotechnology, and Biochemistry

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“…For example, P. ananatis has icenucleation active (ina) genes and E. herbicola pv. gypsophilae has indole-3-acetic acid (IAA) biosynthesis genes consisting of iaaM and iaaH and a cytokinin biosynthesis gene (etz) (Abe et al 1989;Clark et al 1993;Lichter et al 1995;Michigami et al 1994). Therefore, the polymerase chain reaction (PCR) was performed to detect ina, iaaM, iaaH, and etz genes on the tested bacteria.…”

Section: Other Genetic Characterizationsmentioning

confidence: 99%

Internal fruit rot of netted melon caused by Pantoea ananatis (=Erwinia ananas) in Japan

Kido

Adachi²,

Hasegawa³

et al. 2008

J Gen Plant Pathol

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An internal fruit rot with a malodor was found in netted melons (Cucumis melo L.) in commercial greenhouses in Kochi Prefecture, Japan, in 1998, despite their healthy appearance and lack of water-soaking or brown spots on the surface. A yellow bacterium was consistently isolated from the affected fruits. To confirm the pathogenicity of eight representative isolates of the yellow bacterium, we stub-inoculated ovaries (immature-fruits) 5-7 days after artificial pollination, with a pin smeared with bacteria. After the melon fruits had grown for 60 more days, an internal fruit rot resembling the natural infection appeared, and the inoculated bacterium was reisolated. The melon isolates had properties identical with Pantoea ananatis, such as gram-negative staining, facultative anaerobic growth, indole production, phenylalanine deaminase absence, and acid production from melibiose, sorbitol, glycerol, and inositol. Phylogenetic analysis based on 16S rDNA sequences showed that the melon bacterium positioned closely with known P. ananatis strains. The melon bacterium had indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). The bacterium could be distinguished from the other 'Pantoea' group strains by rep-PCR genomic fingerprinting. From these results, the causal agent of internal fruit rot was identified as a strain of P.ananatis [Serrano in (Philipp J Sci 36:271-305, 1928); Mergaert et al. in (Int J Syst

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“…Genes conferring ice-nucleating activity have been sequenced from six bacterial strains, and all encode ice nuclei proteins, INP (120-180 kDa), with similar primary structures (Abe et al, 1989;Green and *Corresponding author . Tel : +81-6-6368-0832, Fax: + 81-6-6388-8609 Warren, 1985;Michigami et al, 1994;Schmid et al, 1997;Warren et al, 1986). However, all the amino acid sequences that were deduced from the DNA sequences have no signal sequences of 15 to 70 amino acid residues that are located at the N-terminus.…”

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confidence: 99%

The Control of the Production and Secretion of Extracellular Ice-nucleating Material of Erwinia uredovora KUIN-3.

Kawahara

Matsushita²,

Yamade³

et al. 1999

Biocontrol Sci.

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Cloning and Sequencing of an Ice Nucleation Active Gene ofErwinia uredovora

Cited by 19 publications

References 2 publications

Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleatingPseudomonas antarcticaIN-74

Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleatingPseudomonas antarcticaIN-74

Internal fruit rot of netted melon caused by Pantoea ananatis (=Erwinia ananas) in Japan

The Control of the Production and Secretion of Extracellular Ice-nucleating Material of Erwinia uredovora KUIN-3.

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