Cloning and Sequencing of Equine Cardiac Troponin I and Confirmation of Its Usefulness as a Target Analyte for Commercial Troponin I Analyzers

Rishniw, Mark; Simpson, K. W.

doi:10.1177/104063870501700611

Cited by 31 publications

(25 citation statements)

References 26 publications

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“…Apart from a unique 59 equine 6-amino-acid N-terminal deletion, human and equine cTnI are largely similar. Since 60 this deletion lies outside the epitope region of most commercial assays, human cTnI assays 61 can be used for detection of myocardial damage in horses (Rishniw and Simpson, 2005 from the ROC curve ( Fig. 1; Table 1); however, the sensitivity was low and the P value was 191 >0.05, which indicates that the assay did not distinguish between groups 1 and 2.…”

mentioning

confidence: 95%

Evaluation of assays for troponin I in healthy horses and horses with cardiac disease

Vekens

Decloedt

Sys

et al. 2015

The Veterinary Journal

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mentioning

confidence: 95%

Evaluation of assays for troponin I in healthy horses and horses with cardiac disease

Vekens

Decloedt

Sys

et al. 2015

The Veterinary Journal

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“…The horse cTnI amino acid sequence is nearly identical to human cTnI, and differences are outside the epitope region used in human commercial analyzers. 29 After administration of monensin, horses were monitored hourly for any abnormal clinical signs (e.g., colic, diarrhea, anorexia, trembling, and ataxia), and heart rates were determined at least every 6 hr. By study design, any horse that developed signs of fulminant heart failure (i.e., coughing, jugular pulses, edema, and dyspnea) was euthanized with a concentrated pentobarbital solution, e and a complete necropsy was performed on each animal.…”

Section: Methodsmentioning

confidence: 99%

Clinical Findings and Serum Cardiac Troponin I Concentrations in Horses after Intragastric Administration of Sodium Monensin

et al. 2009

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Abstract. Six adult horses were administered sodium monensin, 1.0-1.5 mg/kg, via gastric gavage. Anorexia and/or diarrhea occurred within 24 hr after monensin administration in all 6 horses. Cardiac disease and dysfunction were evaluated by both elevations in heart rate, echocardiography, and an increase in serum concentrations of cardiac troponin I (cTnI), occurred in 4 horses. The development and severity of cardiac disease was likely affected by the monensin dose, vehicle (water or corn oil) mixed with monensin, and/or whether the monensin was administered to fed or fasted horses. Initial increases in cTnI concentrations occurred between 24 and 72 hr after monensin administration. The 2 horses with the highest cTnI concentrations died or were euthanized within 5 days after monensin administration and had severe cardiac disease. One horse had increased cTnI concentrations from day 2 to day 16, but no apparent change in ventricular contractile function was evident on echocardiography. The fourth diseased horse did not return to cTnI reference intervals until day 27 after monensin administration, and the ventricular function was still abnormal just before euthanasia 9 months later. Cardiac troponin I measurements could be useful in managing farm outbreaks of accidental monensin feeding by the early identification of horses with cardiac disease.

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“…This ease of determination makes cTnI analysis a potentially useful screening tool for the detection of myocardial injury. The gene sequence of equine cTnI has been shown to be highly homologous to human cTnI, suggesting that monoclonal antibody‐based cTnI assays are likely to detect equine cTnI . However, to the authors' knowledge, rigorous cTnI assay validation according to the American Society of Veterinary Clinical Pathology (ASVCP) quality control principles has not been performed for equine samples and upper reference limit determinations have not been made for a large population of racing Standardbreds.…”

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confidence: 99%

Cardiac Troponin I in Racing Standardbreds

Slack

Boston

Soma

et al. 2012

Veterinary Internal Medicne

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Background Upper reference limits for cTnI have not been established for healthy Standardbred racehorses. Objectives To establish cTnI upper reference limits for Standardbred racehorses and determine if increases in plasma cTnI concentration can be detected in 1–2 hours after a race. Animals Samples were obtained from 586 apparently healthy Standardbreds aged 2–14 years before racing and from the winners of 144 races 1–2 hours after the end of the race. Methods Prospective, observational study; convenience sampling; assay validation; and reference limits determinations were performed according to ASCVP guidelines. Plasma cardiac troponin I concentrations before racing were determined, potential outliers identified, and the 95th and 99th percentile upper reference limits calculated using nonparametric methods. The correlation between cTnI concentration and age, differences in median cTnI concentrations by subgroups and differences between cTnI concentrations before and after racing in winning horses were determined. Results The 95th and 99th percentile upper reference limits for all horses excluding outliers were < 0.04ng/mL and 0.06 ng/mL. There were no significant differences in cTnI concentrations based on age (P = .06), sex (P = .35), gait (P = .55), or race classification (P = .65) and a weak correlation of cTnI with age (ρ = 0.09, P = .03). There were no significant differences between cTnI concentrations before and after racing in winning horses (P = .70). Conclusions and Clinical Importance Because of lack of standardization across cTnI assays, the reference limits apply only to the Stratus CS immunoassay. Future studies looking at the effects of high intensity, short duration exercise on cTnI should consider sampling more than 2 hours after racing or using an ultrasensitive assay.

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Cloning and Sequencing of Equine Cardiac Troponin I and Confirmation of Its Usefulness as a Target Analyte for Commercial Troponin I Analyzers

Cited by 31 publications

References 26 publications

Evaluation of assays for troponin I in healthy horses and horses with cardiac disease

Evaluation of assays for troponin I in healthy horses and horses with cardiac disease

Clinical Findings and Serum Cardiac Troponin I Concentrations in Horses after Intragastric Administration of Sodium Monensin

Cardiac Troponin I in Racing Standardbreds

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