Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase

Marquardt, John L.; Siegele, Deborah A.; Kolter, Roberto; Walsh, Christopher T.

doi:10.1128/jb.174.17.5748-5752.1992

Cited by 132 publications

(131 citation statements)

References 22 publications

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“…femB was also identified by STM, and femB knockout mutants were strongly attenuated in murine infection models (Mei et al, 1997 ;Coulter et al, 1998). Mutant SMI18 contained a transposon insertion in a gene that shares homology to murZ of Bacillus subtilis, which is also involved in peptidoglycan synthesis (Marquardt et al, 1992). After using IVET an S. aureus gene homologous to murC of B. subtilis was identified ; this gene was induced in a murine infection model (Lowe et al, 1998).…”

Section: Cloning and Sequence Analysis Of Flanking Chromosomal Dna Rementioning

confidence: 99%

Identification of Staphylococcus aureus genes expressed during growth in milk: a useful model for selection of genes important in bovine mastitis? The GenBank accession numbers for the sequences determined in this work are AF223893–AF223920.

et al. 2000

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Staphylococcus aureus is a major cause of bovine mastitis. Since gene expression of many bacteria is known to be regulated by the environment, milk may play an important role in the regulation of the early steps in the pathogenesis of bovine mastitis by S. aureus. To get insight into the response of S. aureus to the milk environment, a Tn917-lacZ mutant library was generated and screened for genes specifically expressed during growth in milk. Twenty-eight mutants were identified and analysed. Four groups of genes were found, involved in cell-wall synthesis, nucleotide synthesis, transcriptional regulation and carbohydrate metabolism. A fifth group contained genes with hypothetical or unknown functions. Many of the genes identified belonged to biosynthetic pathways of S. aureus and other bacterial species which have also been shown to play a role in vivo as determined in murine infection models. Therefore, growth on milk may be an attractive model for the identification of genes preferentially expressed during bovine mastitis.

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Section: Cloning and Sequence Analysis Of Flanking Chromosomal Dna Rementioning

confidence: 99%

Identification of Staphylococcus aureus genes expressed during growth in milk: a useful model for selection of genes important in bovine mastitis? The GenBank accession numbers for the sequences determined in this work are AF223893–AF223920.

et al. 2000

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“…For instance, recombinant cells with increased doses of murZ, the E. coli gene encoding UDP-GlcNAc enolpyruvate transferase, displayed phosphomycin resistance (8). Clones resistant to thiolactomycin were found to contain increased levels of fabB, which encodes ␤-ketoacyl-acyl carrier protein synthase I, the cellular target of thiolactomycin (9).…”

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confidence: 99%

A Genetic Screen for the Identification of Thiamin Metabolic Genes

Lawhorn

Gerdes

Begley

2004

Journal of Biological Chemistry

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A genetic screen was developed for the identification of genes related to thiamin biosynthesis and degradation. Genes conferring resistance to bacimethrin or 4-amino-2-trifluoromethyl-5-hydroxymethylpyrimidine were selected from Escherichia coli and Bacillus subtilis genomic libraries. Hits from the selection included the known thiamin biosynthetic genes thiC, thiE, and dxs as well as five genes of previously unknown function (E. coli yjjX, yajO, ymfB, and cof and B. subtilis yveN). The gene products YmfB and Cof catalyze the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate. YmfB also converts thiamin pyrophosphate into thiamin phosphate.

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“…The recent cloning of the murb gene (Pucci et al, 1992) and the availability of the UDPGlcNAcEP substrate from work on the preceding enzyme MurZ (MurA) (Marquardt et al, 1992) allowed overexpression and purification of 100-mg quantities of MurB for biochemical studies (Benson et al, 1993). MurB purified as a monomer with a molecular weight of 35 kDa from gel filtration analysis.…”

mentioning

confidence: 99%

Crystallization and preliminary X‐ray crystallographic studies of UDP‐N‐acetylenolpyruvylglucosamine reductase

1994

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Abstract:The overexpression and purification of the second enzyme in Escherichia coli peptidoglycan biosynthesis, UDP-Nacetylenolpyruvylglucosamine reductase (MurB), provided sufficient protein to undertake crystallization and X-ray crystallographic studies of the enzyme. MurB crystallizes in 14-20% PEG 8000, 100 mM sodium cacodylate, pH 8.0, and 200 mM calcium acetate in the presence of its substrate UDP-Nacetylglucosamine enolpyruvate. Crystals of MurB belong to the tetragonal space group P4,2,2 with a = b = 49.6 A , c = 263.2 A , and cx = / 3 = y = 90" at -160 "C and diffract to at least 2.5 A. Screening for heavy atom derivatives has yielded a single site that is reactive with both methylmercury nitrate and Thimerosal.

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Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase

Cited by 132 publications

References 22 publications

Identification of Staphylococcus aureus genes expressed during growth in milk: a useful model for selection of genes important in bovine mastitis? The GenBank accession numbers for the sequences determined in this work are AF223893–AF223920.

Identification of Staphylococcus aureus genes expressed during growth in milk: a useful model for selection of genes important in bovine mastitis? The GenBank accession numbers for the sequences determined in this work are AF223893–AF223920.

A Genetic Screen for the Identification of Thiamin Metabolic Genes

Crystallization and preliminary X‐ray crystallographic studies of UDP‐N‐acetylenolpyruvylglucosamine reductase

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