Cloning and sequencing of the gene for apocytochrome b of the yeast <i>Kluyveromyces lactis</i> strains WM27 (NRRL Y‐17066) and WM37 (NRRL Y‐1140)

Brunner, Aurora; Coria, Roberto

doi:10.1002/yea.320050310

Cited by 16 publications

(8 citation statements)

References 28 publications

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“…The boundartes of the conserved domains in cytochrome b that were assigned prev]ously [6,27,33] are largely confirmed m the current ahgnment of sequences (Fig 3) We have evaluated the different degree of conservation of the structural elements of the protein by measuring their average score of identity m the ahgnment of This consensus sequence has been generated by using the alignment of Fig 2 normahzed to the yeast sequence (gaps or insertions have been excluded) and extended to the following sequences to increase the variation /~ lac.s [198], C glabrata (G D Clark-Walker, personal communication). N crassa [200], A franclscana (R Garesse and F Marco, personal communication), T brucet [48,49], S purpuratus [45].…”

Section: Iii-c the Most And Least Conserved Regtons In Cytochrome Bmentioning

confidence: 84%

Mitochondrial cytochrome b: evolution and structure of the protein

Esposti

Vries

Crimi

et al. 1993

Biochimica et Biophysica Acta (BBA) - Bioenergetics

376

285

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Section: Iii-c the Most And Least Conserved Regtons In Cytochrome Bmentioning

confidence: 84%

Mitochondrial cytochrome b: evolution and structure of the protein

Esposti

Vries

Crimi

et al. 1993

Biochimica et Biophysica Acta (BBA) - Bioenergetics

376

285

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“…There exist, however, cases of notable polymorphic difference. Coria et al [35,36] and Brunner et al [37] have reported changes of restriction sites and a sequence translocation in the strain WM 27 (a clone of NRRL Y‐1118 = CBS 6315, which is supposed to be one of the parents of the strain W600B, which in turn is the source of ade1 , ade2 and leu1 markers carried by many laboratory strains). These variations may be taken into account in the exploitation of the genomic sequence data of the strain Y‐1140 (CBS2359).…”

Section: Resultsmentioning

confidence: 99%

Complete nucleotide sequence of the mitochondrial DNA from

Zivanovic

Wincker

Vacherie

et al. 2005

FEMS Yeast Research

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The total nucleotide sequence of the mitochondrial genome of the yeast Kluyveromyces lactis was determined. The DNA is a circular molecule of 40,291 base pairs, with 26.1% GC. It contains a set of protein- and RNA-coding genes equivalent to those of the Saccharomyces cerevisiae mitochondrial genome. The genome size is about one half of that of S. cerevisiae mitochondrial DNA. The difference in size is due essentially to a reduced proportion of intergenic and intronic sequences. The coding sequences occupy about one third of the genome, the rest being composed of AT-rich sequences and numerous short GC-rich clusters that are dispersed mostly in the non-coding regions and a few within coding sequences. The presence of these GC clusters is a characteristic feature common to K. lactis and S. cerevisiae mitochondrial DNA, although their sequence patterns are different. The absence of the NADH dehydrogenase subunit genes distinguishes this yeast and S. cerevisiae from the typically aerobic species. The genetic code appears to be that of the standard fungal mitochondrial genomes, with UGA as a tryptophan codon. There are only 22 transfer RNA genes, those corresponding to CUN and CGN codons being missing. CUN codons are absent in the protein-coding sequences. There are five CGN codons within the open reading frames, but they are located exclusively in the introns, rendering them untranslatable. Introns are found only the genes in KlCOX1 and LrRNA. The transcription promoter motif known in S. cerevisiae and several other yeast species is also present. All genes are transcribed from the same strand, except those on a single 7-kilobase pairs segment (EMBL Accession No. AY654900).

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“…However, yeasts show a paucity of mitochondrial genes, which in some species is compensated for by a structural complexity in the intergenic regions, resulting from the distribution of optional introns (Wilson & Fukuhara, 1991 ;Hardy & Clark-Walker, 1991 ;Clark-Walker & Weiller, 1994), rearrangements and\or insertions\deletions that encompass entire genes (Hardy et al, 1989 ;Wilson et al, 1989 ;Hoeben et al, 1993 ;Pis) kur et al, 1998), together with a complex pattern of changes from the universal code of codon usage (Brunner & Coria, 1989 ;ClarkWalker & Weiller, 1994).…”

Section: Belloch and Othersmentioning

confidence: 99%

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Belloch¹,

Querol²,

García³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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A phylogenetic analysis of 17 species belonging to the genus Kluyveromyces and 12 reference and outgroup species was performed using mitochondrial cytochrome-c oxidase II gene sequences. The genus Kluyveromyces appears as a polyphyletic taxon formed by species included within the following four main groups. The Kluyveromyces phaffii group encompasses the species Kluyveromyces blattae, K. phaffii and Kluyveromyces yarrowii. The Kluyveromyces marxianus group is a monophyletic group consisting of the species Kluyveromyces aestuarii, Kluyveromyces dobzhanskii, Kluyveromyces lactis, K. marxianus and Kluyveromyces wickerhamii. The monophyletic Kluyveromyces thermotolerans group is formed by K. thermotolerans, Kluyveromyces waltii and Saccharomyces kluyveri (which appears in the mitochondrial tree as the sister clade of the K. marxianus group). Finally, the Saccharomyces cerevisiae group contains the remaining Kluyveromyces species, as well as the reference Saccharomyces species (sensu lato and sensu stricto) and Candida glabrata (the phylogenetic relationships within this group are unclear according to the bootstrap test). The phylogenetic relationships obtained for this mitochondrial gene are, for the most part, congruent with previous trees based on nuclear rRNA sequences, except for the position of K. yarrowii and the close relationship between the K. marxianus and K. thermotolerans groups. These differences, as well as the existence of these groups, are discussed in the context of previous studies based on phenotypic, genetic and molecular data. Although additional studies are required to decipher the phylogenetic relationships between the genus Kluyveromyces and the closely related genera Saccharomyces, Torulaspora and Zygosaccharomyces, future changes to their taxonomic status should take account of the existence of these four groups of Kluyveromyces species.

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Cloning and sequencing of the gene for apocytochrome b of the yeast Kluyveromyces lactis strains WM27 (NRRL Y‐17066) and WM37 (NRRL Y‐1140)

Cited by 16 publications

References 28 publications

Mitochondrial cytochrome b: evolution and structure of the protein

Mitochondrial cytochrome b: evolution and structure of the protein

Complete nucleotide sequence of the mitochondrial DNA from

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

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