Cloning and sequencing of the <i>URA5</i> gene from the yeast <i>Yarrowia lipolytica</i>

Sánchez, Manuel Ortigueira; Prado, Marciano; Iglesias, Francisco Javier Merchán; Domínguez, Ángel

doi:10.1002/yea.320110505

Cited by 21 publications

(14 citation statements)

References 39 publications

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“…. TTT transcription termination motif (Zaret and Sherman, 1982) is found at the 3' end of most of the genes so far published (Davidow et al, 1987;Xuan et al, 1990;Nuttley et al, 1994;Sanchez et al, 1995). Such motifs were also observed at the 3' end of the L YSl gene (Figure 2, underlined), as well as a putative polyadenylation signal (position + 1161).…”

Section: Analysis Of L Ysl Junking Sequencesmentioning

confidence: 77%

“…To this end, we used a genomic library of Y. lipolytica DNA made in the integrative vector pINA 62 (Xuan et a/., 1988) which carries LEU2 as a selective marker. Transformation of 21601-2 with the library after Not1 digestion (Sanchez et a/., 1995) yielded three Lys+ clones out of 5275 Leut transformants tested. All three transformants exhibited identical hybridization patterns (not shown), and one was chosen for rescuing the insert integrated at the LEU2 locus.…”

Section: Isolation and Nucleotide Sequence Of The L Ysl Genementioning

confidence: 99%

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Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

Pérez-Campo

Nicaud²,

Gaillardin³

et al. 1996

Yeast

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Section: Analysis Of L Ysl Junking Sequencesmentioning

confidence: 77%

Section: Isolation and Nucleotide Sequence Of The L Ysl Genementioning

confidence: 99%

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

Pérez-Campo

Nicaud²,

Gaillardin³

et al. 1996

Yeast

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“…In the 3Ј region, typical terminator consensus sequences, i.e., TAA, TTT, and TAGT (68), were found, in common with other Y. lipolytica genes (57,66). …”

Section: Isolation and Characterization Of Morphological Mutantsmentioning

confidence: 99%

HOY1, a Homeo Gene Required for Hyphal Formation inYarrowia lipolytica

Torres-Guzmán

Domínguez

1997

Molecular and Cellular Biology

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The dimorphic fungus Yarrowia lipolytica grows to form hyphae either in rich media or in media with GlcNAc as a carbon source. A visual screening, called FIL (filamentation minus), for Y. lipolytica yeast growth mutants has been developed. The FIL screen was used to identify three Y. lipolytica genes that abolish hypha formation in all media assayed. Y. lipolytica HOY1, a gene whose deletion prevents the yeast-hypha transition both in liquid and solid media, was characterized. HOY1 is predicted to encode a 509-amino-acid protein with a homeodomain homologous to that found in the chicken Hox4.8 gene. Analysis of the protein predicts a nuclear location. These observations suggest that Hoy1p may function as a transcriptional regulatory protein. In disrupted strains, reintroduction of HOY1 restored the capacity for hypha formation. Northern blot hybridization revealed the HOY1 transcript to be approximately 1.6 kb. Expression of this gene was detected when Y. lipolytica grew as a budding yeast, but an increase in its expression was observed by 1 h after cells had been induced to form hyphae. The possible functions of HOY1 in hyphal growth and the uses of the FIL screen to identify morphogenetic regulatory genes from heterologous organisms are discussed.The yeast-to-hypha morphological transition (dimorphism) is typical of many pathogenic fungi (62). The dimorphic transition is a freely reversible process that can be induced by changes in many parameters (56). Much attention has been focused on Candida albicans as a model for analyzing dimorphism because it is the most frequently isolated fungal pathogen in humans. However, although several groups have reported the isolation of mutants which persist in either the yeast (10) or hyphal (21, 30) form, analysis of these mutants is hampered by the difficulties inherent to genetic manipulations of this asexual diploid organism.Two approaches have been successfully used to carry out the analysis of dimorphism. With one of them, differential hybridization screening (7), several genes that are differentially expressed during morphogenesis have been isolated in C. albicans (i.e., ECE1, expressed in association with cell elongation, and PHR1, which is differentially regulated in response to the pH of the growth medium). Deletion of PHR1 results in a pH-conditional defect in morphogenesis (59).The second approach consists of cloning Candida homologs of Saccharomyces cerevisiae genes known to regulate pseudofilamentous growth. Under conditions of nutrient limitation (i.e., nitrogen starvation), S. cerevisiae undergoes a dimorphic transition to growth of pseudohyphae, linear chains of elongated cells in which the daughters remain attached to the mothers (23, 24). In this context, several C. albicans genes that are members of a Candida map kinase (MAPK) cascade, i.e., CPH1 (40) or ACPR (43), HST7 (11), and CST20 (36, 39), have been isolated by complementation of the corresponding Saccharomyces mutants, i.e., STE12, STE7, and STE20, respectively (11,36,39,40,43). When C. albicans st...

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“…To further extend the data on gene order, sequence comparison, genome compactness and analysis of the terminator-promoter environment among yeast species, we have chosen a different approach: the sequencing of larger fragments of DNA (8-16 kb) from several yeast species. This study reports the sequence of a 10.2 kb Y. lipolytica region containing in its central part two genes previously characterized by us: YlURA5 and YlSEC65 (Sá nchez et al, 1995(Sá nchez et al, , 1997. The two genes are adjacent, with an inverted orientation.…”

Section: Introductionmentioning

confidence: 95%

“…The YlURA5 and YlSEC65 genes were isolated as plasmid pMP47 (Sá nchez et al, 1995). This plasmid contains a 10.2 kb insert from a DNA library constructed from Y. lipolytica W29 strain partially digested with Sau3A and cloned in the BamHI site of the pINA62 vector (Xuan et al, 1988).…”

Section: Plasmids and Strainsmentioning

confidence: 99%

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

Sánchez

Domínguez

2001

Yeast

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We have determined the sequence of a 10275 bp DNA segment of Yarrowia lipolytica located on chromosome VI. The sequence contains six complete open reading frames (ORFs) longer than 100 amino acids and two more partial ORFs at both ends. Two of the ORFs encode for the well-characterized genes YlURA5 (orotate phosphoribosyltransferase) and YlSEC65 (encoding a subunit of the signal recognition particle). These two genes show an identical organization-located on opposite strands and in opposite orientations-in four yeast species: Saccharomyces cerevisiae, Kluyveromyces lactis, Candida albicans and Y. lipolytica. One ORF and the two partial ORFs code for putative proteins showing significant homology with proteins from other organisms. YlVI-108w (partial) and YlVI-103w show 39% and 54% identity, respectively, with YDR430c and YHR088w from S. cerevisiae. YlVI-102c (partial) shows significant homology with a matrix protein, lustrin A from Haliotis rufescens, and with the PGRS subfamily (Gly-rich proteins) of Mycobacterium tuberculosis. The three remaining ORFs show weak or nonsignificant homology with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AI006754.

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Cloning and sequencing of the URA5 gene from the yeast Yarrowia lipolytica

Cited by 21 publications

References 39 publications

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

HOY1, a Homeo Gene Required for Hyphal Formation inYarrowia lipolytica

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

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