Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus

Akiyoshi, Donna E.; Chakerian, R.; Rohrmann, George F.; Nesson, Michael H.; Beaudreau, G. S.

doi:10.1016/0042-6822(85)90267-3

Cited by 49 publications

(20 citation statements)

References 9 publications

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“…Hybridization was carried out in rapid hybridization buffer (Amersham) at 65 °C for 2 to 3 h and the membrane was washed under conditions recommended by the supplier. To define the zero point of the physical map, the granulin gene was located by hybridization to a labelled clone containing the granulin gene of Trichoplusia ni GV (TnGV) (Akiyoshi et al, 1985), a generous gift from Dr Y. Hashimoto.…”

Section: Methodsmentioning

confidence: 99%

Restriction endonuclease analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species

Goto¹,

Minobe²,

Iizuka³

1992

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Restriction endonuclease analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species

Goto¹,

Minobe²,

Iizuka³

1992

Journal of General Virology

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“…A SalI fragment of T. ni GV DNA containing the entire granulin gene (Akiyoshi et al, 1985) was labelled with 3zp by nick translation (Maniatis et al, 1982) and allowed to hybridize to blots of CpGV DNA fragments (Howley et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

“…The most appropriate zero point for maps of GV DNAs is therefore the position of the granulin gene. This was located on the CpGV map using a cloned T. ni GV DNA Sail fragment (Akiyoshi et al, 1985), containing the entire 742 bp granulin gene, as a probe. With an EcoRI digest of CpGV-M DNA, the 3~p-labelled probe hybridized to fragments L and also one or more of G, H and I; with a BamHI digest, the probe hybridized to fragments K and I (Fig.…”

Section: Orientation Of Cpg V Mapmentioning

confidence: 99%

Variation in Cydia pomonella Granulosis Virus Isolates and Physical Maps of the DNA from Three Variants

Crook

Spencer

Payne

et al. 1985

Journal of General Virology

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SUMMARYCydia pomonella granulosis viruses (CpGV) from seven different sources in Europe, America and New Zealand were compared by restriction enzyme analysis. Most samples were indistinguishable from the Mexican isolate (CpGV-M). Isolates from Russia (CpGV-R) and England (CpGV-E) showed small genotypic differences. CpGV-E was shown to be a mixture of two variants, E1 and E2. CpGV-E1 was indistinguishable from CpGV-M. A physical map of CpGV-M was constructed for the enzymes EcoRI, BamHI, HindIII, Sinai and ApaI. A comparison of fragment profiles allowed construction of maps for CpGV-R and CpGV-E2. Relative to CpGV-M, CpGV-R had a single deletion of 2.4 kbp and CpGV-E2 was modified in one area resulting in an additional EcoRI site, a shift in a BamHI site and in total about 1 kbp more DNA. The map was orientated by locating the granulin gene using the cloned granulin gene from Trichoplusia ni GV as a probe. There was no significant difference between the infectivities of the Mexican, Russian and English isolates for neonate larvae.

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“…4). This sequence appears to be conserved throughout the Baculoviridae: related sequences have been described upstream of granulin genes (Akiyoshi et al, 1985;Chakerian et al, 1985), AcMNPV 10K (Kuzio et al, 1984) and OpMNPV 10K (D. Leisy et al, unpublished observations) protein genes and the polyhedrin genes of AcMNPV (Hooft van Iddekinge et al, 1983), Bombyx mori NPV (BmNPV) (Iatrou et al, 1985) and OpMNPV (D. Leisy et al, unpublished observations). All hypertranscribed baculovirus genes examined (polyhedrin and 10K), have mRNA initiation sites at or very near this conserved sequence (Hooft van Iddekinge et al, 1983;Kuzio et al, 1984;D.…”

Section: Genome Map and Location Of The Polyhedrin Genementioning

confidence: 99%

Location and Nucleotide Sequence of the Orgyia pseudotsugata Single Nucleocapsid Nuclear Polyhedrosis Virus Polyhedrin Gene

Leisy

Nesson

Pearson

et al. 1986

Journal of General Virology

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SUMMARYA restriction endonuclease map was determined for the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus (SNPV) genome. The order of the fragments generated by the enzymes BglII, BamHI and XbaI was analysed using double digestion of the total genome and digestion of isolated restriction fragments. The location of the polyhedrin gene was then determined using a cloned polyhedrin gene from the O. pseudotsugata multiple nucleocapsid NPV (MNPV) as a hybridization probe. A fragment containing this gene was cloned, mapped, subcloned and the nucleotide sequence of a 1.3 kb fragment was determined which contained the entire polyhedrin reading frame and some flanking sequences. This gene demonstrated 76% nucleotide sequence homology and 87% amino acid sequence homology to the Autographa californica MNPV polyhedrin sequence. A probable regulatory element was identified which is common to the 5' flanking region of all hypertranscribed late genes (polyhedrin and 10K proteins) which have been examined in baculoviruses.

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Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus

Cited by 49 publications

References 9 publications

Restriction endonuclease analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species

Restriction endonuclease analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species

Variation in Cydia pomonella Granulosis Virus Isolates and Physical Maps of the DNA from Three Variants

Location and Nucleotide Sequence of the Orgyia pseudotsugata Single Nucleocapsid Nuclear Polyhedrosis Virus Polyhedrin Gene

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