Cloning and transcript analyses of the chimeric gene associated with cytoplasmic male sterility in cauliflower (Brassica oleracea var. botrytis)

Wang, Chunguo; Chen, Xiaoqiang; Lan, Tianying; Li, Hui; Song, Wen-qin

doi:10.1007/s10681-006-9268-3

Cited by 3 publications

(5 citation statements)

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“…The CMS-inducing gene orf138 produces a 19-kDa mitochondrial-membrane protein which is distinctly reduced in the flowers and leaves, but not in the roots of restorer plants (Bonhomme et al 1991;Grelon et al 1994;Krishnasamy and Makaroff 1993;Wang et al 2006a). These results indicate that the Rf gene has no ability to block orf138 transcription, but that it may affect it at the translational or post-translational level and in an organ-specific manner (Krishnasamy and Makaroff 1993;Bellaoui et al 1997;Wu et al 2001;Wang et al 2006a).…”

Section: Discussionmentioning

confidence: 92%

“…These results indicate that the Rf gene has no ability to block orf138 transcription, but that it may affect it at the translational or post-translational level and in an organ-specific manner (Krishnasamy and Makaroff 1993;Bellaoui et al 1997;Wu et al 2001;Wang et al 2006a). The results of the expression analysis which we performed in our CMS-Rf system indicate that the orf138 gene was not expressed at all three stages of anther development in restorer plants, while in F 1 and CMS plants, its expression level was up-regulated, with the expression level being relatively lower in F 1 plants than in CMS plants.…”

Section: Discussionmentioning

confidence: 95%

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Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

et al. 2012

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Cytoplasmic male sterility (CMS) is a maternally inherited trait that is widely utilized in hybrid seed production. In this study, the Rs-Rf 1 gene and its allele, the Rs-rf 1 gene, were isolated from a radish CMS restorer line and maintainer, respectively. The Rs-Rf 1 gene was identified as a member of the pentatricopeptide repeat family. The results of reverse transcription (RT)-PCR and quantitative RT-PCR analyses of the floral buds at different development stages were used to characterize the expression features of Rs-Rf 1 and orf138, a unique transcribed gene in the mitochondrion for its role in determining male sterility in the cytoplasm. orf138 was upregulated in the CMS line and F 1 hybrid at three bud developmental stages, including meiosis and the tetroid and microspore stages, but it was not expressed in the maintainer and restorer line, suggesting that the Rs-Rf 1 gene was not able to block orf138 transcription. As in the F 1 plant, the transcription of Rs-Rf 1 was gradually up-regulated in the restorer line with progressive stages of bud development, but it was not transcribed in the CMS line and maintainer. Different restriction sites for SspI between the allele of Rs-Rf 1 and Rs-rf 1 were also identified, and a guideline for using the functional marker to distinguish the genotypes of individuals with Rs-Rf 1 was developed. Due to its co-dominant character, this marker could be used to discriminate the heterozygous and homozygous fertility-restoring allele. Using this functional marker, we established a system of molecular marker-assisted development of elite CMS lines in radish. This system will contribute greatly to candidate maintainer identification and accelerate the process of elite CMS line development in late-bolting radish breeding programs.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

et al. 2012

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“…For example, heat stress affects pollen and flower sterility [ 66 ]. Of the 34 candidate genes, four were found to have key roles in gametogenesis [ 67 ], pollen development [ 68 , 69 ], or Ogura cytoplasmic male sterility [ 70 ]. Heat-stress also causes cellular damage by oxidative stress and toxicity due to reactive oxygen species (ROS) formation [ 71 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

QTL-Seq identifies quantitative trait loci of relative electrical conductivity associated with heat tolerance in bottle gourd (Lagenaria siceraria)

Song

Huang

2020

PLoS ONE

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Heat is a major abiotic stress that seriously affects watermelon (Citrullus lanatus) production. However, its effects may be mitigated through grafting watermelon to heat tolerant bottle gourd (Lagenaria siceraria) rootstocks. Understanding the genetic basis of heat tolerance and development of reliable DNA markers to indirectly select for the trait are necessary in breeding for new varieties with heat tolerance. The objectives of this study were to investigate the inheritance of heat tolerance and identify molecular markers associated with heat tolerance in bottle gourd. A segregating F2 population was developed from a cross between two heat tolerant and sensitive inbred lines. The population was phenotyped for relative electrical conductivity (REC) upon high temperature treatment which was used as an indicator for heat tolerance. QTL-seq was performed to identify regions associated with heat tolerance. We found that REC-based heat tolerance in this population exhibited recessive inheritance. Seven heat-tolerant quantitative trait loci (qHT1.1, qHT2.1, qHT2.2, qHT5.1, qHT6.1, qHT7.1, and qHT8.1) were identified with qHT2.1 being a promising major-effect QTL. In the qHT2.1 region, we identified three non-synonymous SNPs that were potentially associated with heat tolerance. These SNPs were located in the genes that may play roles in pollen sterility, intracellular transport, and signal recognition. Association of the three SNPs with heat tolerance was verified in segregating F2 populations, which could be candidate markers for marker assisted selection for heat tolerance in bottle gourd. The qHT2.1 region is an important finding that may be used for fine mapping and discovery of novel genes associated with heat tolerance in bottle gourd.

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“…н.) (Wang et al, 2006). Маркерный фрагмент 300 п. н. присутствовал у тестируемых образцов с цитоплазмой Ogura, однако фрагмент размером 600 п. н.…”

Section: тип цитоплазмы Oguraunclassified

“…Праймеры P21 и P32, которые являются специфичными для гена orf224, обусловливающего Polima-ЦМС (Wei et al, 2005;Wang et al, 2006), амплифицировали фрагмент 747 п. н. у тех же образцов, что и праймеры pol-5′ и pol-3′, подтверждая тип цитоплазмы Polima.…”

Section: нехромосомная наследственностьunclassified

Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers

et al. 2015

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Cloning and transcript analyses of the chimeric gene associated with cytoplasmic male sterility in cauliflower (Brassica oleracea var. botrytis)

Abstract: The online version of the original article can be found at http://dx.

Cited by 3 publications

References 0 publications

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

QTL-Seq identifies quantitative trait loci of relative electrical conductivity associated with heat tolerance in bottle gourd (Lagenaria siceraria)

Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers

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