Cloning and transcript analysis of type 2 metallothionein gene (SbMT-2) from extreme halophyte Salicornia brachiata and its heterologous expression in E. coli

Chaturvedi, Amit K.; Mishra, Avinash; Tiwari, Vivekanand; Jha, Bhavanath

doi:10.1016/j.gene.2012.03.001

Cited by 96 publications

(58 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…antiporter activities between the halophyte Suaeda salsa and three glycophytes. Like model plant systems and several yeast mutants, some recent studies have proved that E. coli cells can be used for functional validation of plant stress tolerance genes [53][54][55][56]. In the present study, RO/NtP5CS-transformed E. coli grew better cells than did RO/AtP5CS-transformed cells when subjected to salt, alkali, drought, osmotic, heat or cold stress.…”

Section: Discussionsupporting

confidence: 48%

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng

Dang

et al. 2013

Mol Biol Rep

View full text Add to dashboard Cite

Several functional and regulatory proteins play important roles in controlling plant stress tolerance. Proline (Pro) is one of the most accumulated osmolytes correlated with tolerance to stresses. Δ(1)-Pyrroline-5-carboxylate synthetase (P5CS) is a rate-limiting enzyme in Pro biosynthesis. In the present study, we isolated the cDNA for a P5CS gene (NtP5CS) from the halophyte Nitraria tangutorum. Phylogenetic analysis and subcellular localization analysis of NtP5CS-GFP protein in onion cells showed that NtP5CS was a new P5CS gene and was involved in Pro synthesis in N. tangutorum. Expression of the NtP5CS gene was induced by salt stress, dehydration, and high and low temperatures. Escherichia coli overexpressing AtP5CS or NtP5CS exhibited better growth in all treatments, including high salinity, high alkalinity, dehydration, osmotic, heat and cold stresses. Additionally, NtP5CS recombinant E. coli cells grew better than did AtP5CS recombinant cells in response to abiotic stresses. Our data demonstrate that the P5CS from a halophytic species functions more efficiently than its homologue from a glycophytic species in improving the stress tolerance of E. coli.

show abstract

Section: Discussionsupporting

confidence: 48%

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng

Dang

et al. 2013

Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…As in model plant systems and several yeast mutants, E. coli may be used to validate the function of plant stress tolerance genes (Gupta et al, 2010;Song and Ahn, 2011;Chaturvedi et al, 2012). The overexpression of Lycoris radiata S-adenosylmethionine synthetase confers growth advantages to an engineered E. coli strain at high salt concentrations .…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is similar to CIPK2 proteins from other plants, and contains conserved domains and motifs. The promoter of NtCIPK2 harbors many cis-acting elements that might be recognized and bound by transcription factors that are related to hormones and stress responses. NtCIPK2 was ubiquitously and robustly expressed in all tested organs, and was induced by salinity, drought, heat, and cold stress. The overexpression of NtCIPK2 in Escherichia coli caused better growth against high salinity, alkalinity, and osmotic conditions, dehydration, and extreme temperatures (i.e., heat and cold) compared to the control. 4717©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4716-4728 (2014) Cloning and characterization of the NtCIPK2 gene Thus, NtCIPK2 is a candidate gene that might improve the stress tolerance of crops and herbs through genetic manipulation.

show abstract

“…Some species of Salicornia have shown important biological properties, such as antioxidant, anti-inflammatory, hypoglycemic and cytotoxic activities (Isca, Seca, Pinto, & Silva, 2014). Furthermore, Salicornia is being widely explored for abiotic stress-responsive genes and promoters to develop transgenic plants that can withstand adverse environmental conditions (Chaturvedi, Mishra, Tiwari, & Jha, 2012;Chaturvedi, Patel, Mishra, Tiwari, & Jha, 2014;Jha, Sharma, & Mishra, 2011;Singh, Mishra, & Jha, 2014a, 2014bUdawat, Mishra, & Jha, 2014). No information is available, so far, on the metabolomics of this important succulent halophytic plant.…”

Section: Introductionmentioning

confidence: 99%

Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

Mishra

Patel

Jha

2015

Journal of Functional Foods

Self Cite

View full text Add to dashboard Cite

Cloning and transcript analysis of type 2 metallothionein gene (SbMT-2) from extreme halophyte Salicornia brachiata and its heterologous expression in E. coli

Cited by 96 publications

References 52 publications

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

Contact Info

Product

Resources

About