Cloning, characterisation and expression of Aeromonas hydrophila major adhesin

Enhancement of protective immunity in European eel (<italic>Anguilla anguilla</italic>) against <italic>Aeromonas hydrophila</italic> and <italic>Aeromonas sobria</italic> by a recombinant <italic>Aeromonas</italic> outer membrane protein

“…All the results showed that the rGST-His tag did not affect the immunogenicity and specificity of the rOmp-G. At the same time, the results also showed that the rOmp-G was recognized by the rabbit antisera ( Table 2), as well as by the eel anti-sera (Fig. 6), suggesting that the rOmp-G has contributed to some conserved epitopes of these three Aeromonas pathogens, and is involved in their immunogenicity [16,[21][22]24,26]. In addition, the S-rOmp-G agglutinated with the whole cells of the three bacteria, suggesting that the specific antibody induced by the rOmp-G can recognize the conserved OMP epitopes on the cell surface of the three Aeromonas pathogens and may provide cross-protective immunogenicity [21].…”

“…Recently, the antigenic cross-reactivity and crossprotection have also been reported in some OMPs, for instance, a conserved 43-kDa OMP from A. hydrophila confers significant protection to fish against two different serotypes of A. hydrophila challenge [21], and a conserved 37 kDa OMP from E. tarda significantly provides protection against the infections by three different serotypes of E. tarda in fish [24]. Up to now, there are no reports about whether a recombinant conserved OMP can provide protection against different pathogenic species.…”

“…The OMPs can induce specific bactericidal antibodies [8][9][10], inhibit the bacterial colonization in hosts [11], and induce cell-mediated immunity [12][13][14]. Many OMPs from different pathogenic bacteria, such as Aeromonas salmonicida [8, [15][16][17], Aeromonas hydrophila [9,[18][19][20][21], Vibrio parahaemolyticus [22], Vibrio alginolyticus [23], Vibrio harveyi [12 -13], Edwardsiella ictaluri [10], and Edwardsiella tarda [14,24], have been identified as effective protective antigens. Some OMPs mixtures extracted from one pathogenic bacterial strain have been proved to provide cross protection for fish against several other bacterial strains or species [15,20], suggesting that different pathogenic bacterial species may share some similar or conserved OMP antigens.…”

“…Recently, further studies have shown that different bacterial strains or species share OMPs with the same molecule weight, which have good immunogenicity to the host [16 -17,21,24 -28]. Moreover, some of these OMPs have been proved to have effective protections for fish against the corresponding bacterial species [16] or other different serotypes strains [17,21,24], which indicates that such OMPs from different pathogens might be used to develop a vaccine for simultaneously preventing the diseases caused by different pathogenic bacteria.…”

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

“…Fish species that are affected by A. hydrophila include freshwater fish; tilapia, catfish and common carp (Ullal et al, 2008;Abd-El-Rhman, 2009;Yin et al, 2009) and brackish and marine water fish; Meagre, Grouper, Sea bream and Mullet (El-Barbary, 2010 a,b). Although A. hydrophila is commonly considered as a secondary pathogen associated with disease outbreaks, it could also become a primary pathogen, causing outbreaks in fish farms with high mortality rates, resulting in severe economic losses to the aquaculture sector worldwide (Nielsen et al, 2001;Fang et al, 2004). The symptoms of A. hydrophila infection include swollen tissues, ascites, necrosis, red sores, ulceration, and hemorrhagic septicemia (Karunasagar et al, 1989;Azad et al, 2001).…”