Cloning, characterization and expression analysis of porcine microRNAs

Reddy, Alavala Matta; Zheng, Yun; Jagadeeswaran, Guru; Macmil, Simone; Graham, Wiley B; Roe, Bruce A.; DeSilva, Udaya; Zhang, Weixiong; Sunkar, Ramanjulu

doi:10.1186/1471-2164-10-65

Cited by 98 publications

(82 citation statements)

References 64 publications

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“…The lack of correlation between sequencing and northern blot results for miR396 expression in stressed J. pentantha could not be attributed to the source of RNA as same RNA samples were used for sequencing and Northern blot analysis. This lack of correlation between sequencing-based profiling and Northern profiling may largely be attributed to the biased-ligation with the adapters (Reddy et al 2009) or sequencing problems. Although miR393 was not found as differentially regulated by drought as per our cut off criterion, the small RNA blot analysis revealed its induction by drought in J. pentantha but not in I. campanulata (Fig.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA dynamics in a wild and cultivated species of Convolvulaceae exposed to drought stress

Ghorecha

Zheng

Liu

et al. 2017

Physiol Mol Biol Plants

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Agricultural productivity is severely hampered by drought in many parts of the globe. It is well-known that wild plant species can tolerate drought better when compared with their closely related cultivated plant species. Better drought adaptation of wild species over cultivated ones is accounted for their ability to differentially regulate gene expression. miRNAs, known to regulate gene expression at the post-transcriptional level, are admitted to play an important role in plant adaptation to stresses. This study aims at evaluating miRNA dynamics in a droughttolerant wild Ipomoea campanulata L. and drought-sensitive cultivated Jacquemontia pentantha (Jacq.) of the family Convolvulaceae under ex situ drought. Sequencing profiles revealed that 34 conserved miRNA families were analogous between the two species. Drought altered expression levels of several of these miRNAs in both the species. Drought-tolerant I. campanulata showed upregulation of miR398, miR168, miR858, miR162 and miR408, while miR394 and miR171 were downregulated. Droughtsensitive J. pentantha showed upregulation of miR394, miR156, miR160, miR164, miR167, miR172, miR319, miR395, miR396, miR403 and downregulation of miR157. Basal miRNA levels and their drought mediated regulation were very different between the two species. Differential drought sensitivities of these two plant species can be attributed to these innate variations in miRNA levels and their expression.

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Section: Resultsmentioning

confidence: 99%

MicroRNA dynamics in a wild and cultivated species of Convolvulaceae exposed to drought stress

Ghorecha

Zheng

Liu

et al. 2017

Physiol Mol Biol Plants

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“…To date, very few miRNAs have been shown to play important roles in HSC development in vertebrates in vivo. miR-142-3p is specifically expressed in HSCs (AGM and CHT) and T cells (thymus) in zebrafish, mouse and other species [15][16][17][18][19][20][21]. By knockdown of miR-142a-3p using 2 specific MOs, the numbers of HSCs and T cells were decreased.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, miR-144 and miR-451, as direct targets of the critical hematopoietic master regulator GATA1, are required for erythropoiesis in zebrafish and mouse [11][12][13]. miR-142-3p is an evolutionally conserved miRNA of vertebrates, which is expressed in many different hematopoietic cells [14][15][16][17][18][19][20][21]. Chen et al [15] first reported that miR-142 is expressed in embryonic and adult hematopoietic tissues, including the fetal liver, bone marrow, spleen and thymus, in mice, indicating its role in both embryonic and adult hematopoiesis.…”

Section: Introductionmentioning

confidence: 99%

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

et al. 2013

Cell Res

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Previous studies on developmental hematopoiesis have mainly focused on signaling and transcription factors, while the appreciation of epigenetic regulation including that of microRNAs is recent. Here, we show that in zebrafish and mouse, miR-142-3p is specifically expressed in hematopoietic stem cells (HSCs). Knockdown of miR-142a-3p in zebrafish led to a reduced population of HSCs in the aorta-gonad-mesonephros (AGM) region as well as T-cell defects in the thymus. Mechanistically, miR-142a-3p regulates HSC formation and differentiation through the repression of interferon regulatory factor 7 (irf7)-mediated inflammation signaling. Finally, we show that miR-142-3p is also involved in the development of HSCs in mouse AGM, suggesting that it has a highly conserved role in vertebrates. Together, these findings unveil the pivotal roles that miR-142a-3p plays in the formation and differentiation of HSCs by repressing irf7 signaling.

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“…Our result suggested that ame-mir-2-1 and -13 are co-expressed and have the same function in the honeybee brain. The nucleotide lengths of ame-mir-2-1, -8, -13a, -34, -276, and -1000 were 1 to 2 nt shorter or longer at the 3 region than those of the registered sequences, which is often observed among various microRNA (Obernosterer et al, 2006;Long and Chen, 2009;Reddy et al, 2009). There are at least two possible explanations; one is that microRNA size actually varies in vivo, and the other is that either the microRNAs registered in the miRBase or isolated in our study represent partly degraded products.…”

Section: Cloning Of Micrornas From the Honeybee Brainmentioning

confidence: 87%

Expression of two microRNAs, ame-mir-276 and -1000, in the adult honeybee (Apis mellifera) brain

et al. 2011

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-To identify candidate microRNAs involved in post-transcriptional regulation of brain (region)-selective gene expression in the adult honeybee brain, we isolated eight microRNAs: seven known microRNAs, ame-mir-2-1, -8, 13a, -34, -276, -317, -1000, and one novel one, named mir-hbd, that has significant sequence similarity with the Drosophila dme-mir-11. Among them, ame-mir-1000 and -276 were expressed in a brain-selective and -preferential manner, respectively, in workers and drones. In particular, ame-mir-276-expression was enriched in the optic lobes and in the small type-Kenyon cells of the mushroom bodies in the nurse bee, forager, queen, and drone brains. Almost all predicted targets of amemir-1000 and -276 encode neural function related genes, suggesting the involvement in neural function of both microRNAs.brain / microRNA / Apis mellifera / honey bee / gene expression

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Cloning, characterization and expression analysis of porcine microRNAs

Cited by 98 publications

References 64 publications

MicroRNA dynamics in a wild and cultivated species of Convolvulaceae exposed to drought stress

MicroRNA dynamics in a wild and cultivated species of Convolvulaceae exposed to drought stress

miR-142-3p regulates the formation and differentiation of hematopoietic stem cells in vertebrates

Expression of two microRNAs, ame-mir-276 and -1000, in the adult honeybee (Apis mellifera) brain

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