Cloning, Characterization, and Transcription of Three Laccase Genes from
            <i>Gaeumannomyces graminis</i>
            var.
            <i>tritici</i>
            , the Take-All Fungus

Litvintseva, Anastasia P.; Henson, Joan M.

doi:10.1128/aem.68.3.1305-1311.2002

Cited by 79 publications

(74 citation statements)

References 58 publications

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“…The occurrence of multiple laccase genes in one organism and their differential regulation in response to numerous external factors and the developmental stage are widely known from terrestrial asco-and basidiomycetes (10,18,31,33). The present study on C. aquatica thus expands the knowledge to ascomycete-related freshwater fungi.…”

Section: Discussionmentioning

confidence: 77%

“…Enhanced extracellular laccase activity in the presence of zinc has also been reported (6,22). Many natural and xenobiotic aromatic compounds, which are often structurally related to lignin or humic substances, were shown to induce laccase gene transcription in terrestrial basidio-and ascomycetes (18,33,44,48). The endocrine disrupting chemical (EDC) nonylphenol is an example of a xenobiotic compound where laccase has been implicated in its fungal degradation (12,29).…”

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confidence: 99%

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Differential Regulation by Organic Compounds and Heavy Metals of Multiple Laccase Genes in the Aquatic Hyphomycete Clavariopsis aquatica

Solé

Müller

Pecyna

et al. 2012

Appl Environ Microbiol

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ABSTRACTTo advance the understanding of the molecular mechanisms controlling microbial activities involved in carbon cycling and mitigation of environmental pollution in freshwaters, the influence of heavy metals and natural as well as xenobiotic organic compounds on laccase gene expression was quantified using quantitative real-time PCR (qRT-PCR) in an exclusively aquatic fungus (the aquatic hyphomyceteClavariopsis aquatica) for the first time. Five putative laccase genes (lcc1tolcc5) identified inC. aquaticawere differentially expressed in response to the fungal growth stage and potential laccase inducers, with certain genes being upregulated by, e.g., the lignocellulose breakdown product vanillic acid, the endocrine disruptor technical nonylphenol, manganese, and zinc.lcc4is inducible by vanillic acid and most likely encodes an extracellular laccase already excreted during the trophophase of the organism, suggesting a function during fungal substrate colonization. Surprisingly, unlike many laccases of terrestrial fungi, none of theC. aquaticalaccase genes was found to be upregulated by copper. However, copper strongly increases extracellular laccase activity inC. aquatica, possibly due to stabilization of the copper-containing catalytic center of the enzyme. Copper was found to half-saturate laccase activity already at about 1.8 μM, in favor of a fungal adaptation to low copper concentrations of aquatic habitats.

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Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

Differential Regulation by Organic Compounds and Heavy Metals of Multiple Laccase Genes in the Aquatic Hyphomycete Clavariopsis aquatica

Solé

Müller

Pecyna

et al. 2012

Appl Environ Microbiol

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“…5). A mixture of Cu 2+ , which is known to induce differential laccase gene expression in asco-and basidiomycetes (Litvintseva & Henson, 2002;Soden & Dobson, 2001), and the lignin-related compound vanillic acid enhanced laccase production in both fungi investigated (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

“…C. aquatica has been connected to ascomycetous teleomorphs (Nikolcheva & Bärlocher, 2004;Webster, 1992). In ascomycetes, laccases have frequently been described (Kiiskinen et al, 2002;Litvintseva & Henson, 2002;Saito et al, 2003). Fungi growing on plant material in aquatic environments would be expected to produce laccase under natural conditions; this could also apply to C. aquatica, which did not excrete substantial amounts of laccase under the nonylphenol-degradation conditions employed within the present study (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The concomitant occurrence of different laccase forms as observed (Fig. 6) is a well-known phenomenon in filamentous fungi and may be attributed to multiple laccase genes or post-translational modification (Litvintseva & Henson, 2002;Palmieri et al, 2003;Thurston, 1994). C. aquatica has been connected to ascomycetous teleomorphs (Nikolcheva & Bärlocher, 2004;Webster, 1992).…”

Section: Discussionmentioning

confidence: 99%

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Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

et al. 2005

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Degradation of technical nonylphenol (t-NP), known as an endocrine-disrupting compound mixture, was assessed, using the mitosporic fungal strain UHH 1-6-18-4 isolated from nonylphenol-contaminated river water, and a strain of the aquatic hyphomycete Clavariopsis aquatica. GC-MS analysis could resolve 12 peaks attributable to nonyl chain-branched t-NP isomers. All were degraded, to individual extents. Analysis of degradation metabolites suggested intracellular hydroxylation of the nonyl moieties of individual t-NP isomers. Further metabolites also indicated shortening of branched nonyl chains, and 4-hydroxybenzoic acid was identified as a t-NP breakdown product in UHH 1-6-18-4. The t-NP degradation efficiency was higher in UHH 1-6-18-4 than in C. aquatica, and a lower specificity in degradation of individual t-NP constituents in UHH 1-6-18-4 than in C. aquatica was observed. Strain UHH 1-6-18-4 concomitantly produced extracellular laccase under degradation conditions. A mixture of CuSO 4 and vanillic acid considerably enhanced laccase production in both fungi. Laccase preparations derived from UHH 1-6-18-4 and C. aquatica cultures also converted t-NP. Laccase-catalysed transformation of t-NP led to the formation of products with higher molecular masses than that of the parent compound. These results emphasize a role of fungi occurring in aquatic ecosystems in degradation of water contaminants with endocrine activity, which has not previously been considered. Furthermore, the results are in support of two different mechanisms employed by fungi isolated from aquatic environments to initiate t-NP degradation: hydroxylation of individual t-NP isomers at their branched nonyl chains and further breakdown of the alkyl chains of certain isomers; and attack of t-NP by extracellular laccase, the latter leading to oxidative coupling of primary radical products to compounds with higher molecular masses. INTRODUCTIONNonylphenols have increasingly gained attention because of their potential to mimic the action of natural hormones in vertebrates (Ying et al., 2002). They result from incomplete biodegradation of nonylphenol polyethoxylates (NPEOs), which have been widely used as non-ionic surfactants in industrial processes and households (Braun et al., 2003;Ying et al., 2002). Both nonylphenols and NPEOs are discharged into the environment, mainly due to incomplete removal in wastewater treatment facilities (Ying et al., 2002). Nonylphenols are more resistant to biodegradation than their parent compounds and hence are found worldwide in wastewater treatment plant effluents and rivers in concentrations of up to the mg l 21 range (Heemken et al., 2001;Kolpin et al., 2002;Stachel et al., 2003;Ying et al., 2002). Due to their hydrophobicity, they tend to adsorb onto surface water particles and sediments and accumulate in aquatic organisms (Heemken et al., 2001;Ying et al., 2002). Consequently, nonylphenols represent a serious environmental and human health risk. The assessment of biodegradative processes affecting the environmental...

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The Biocontrol BacteriumPseudomonas FluorescensPf29Arp Strain Affects the Pathogenesis‐Related Gene Expression of the Take‐All FungusGaeumannomyces GraminisVar.Triticion Wheat Roots

Daval

Lebreton

Sarniguet

2013

Molecular Microbial Ecology of the Rhizosphere

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Cloning, Characterization, and Transcription of Three Laccase Genes from Gaeumannomyces graminis var. tritici , the Take-All Fungus

Cited by 79 publications

References 58 publications

Differential Regulation by Organic Compounds and Heavy Metals of Multiple Laccase Genes in the Aquatic Hyphomycete Clavariopsis aquatica

Differential Regulation by Organic Compounds and Heavy Metals of Multiple Laccase Genes in the Aquatic Hyphomycete Clavariopsis aquatica

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

The Biocontrol BacteriumPseudomonas FluorescensPf29Arp Strain Affects the Pathogenesis‐Related Gene Expression of the Take‐All FungusGaeumannomyces GraminisVar.Triticion Wheat Roots

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