Cloning endangered felids using heterospecific donor oocytes and interspecies embryo transfer

Gómez, M. C.; Pope, C. E.; Ricks, David M.; Lyons, Justine; Dumas, C.; Dresser, B. L.

doi:10.1071/rd08222

Cited by 58 publications

(43 citation statements)

References 37 publications

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“…Incompatibility between the nuclear and mitochondrial genome was reported to have compromised mitochondrial generation of ATP leading to embryonic arrest (Lanza et al 2000, Loi et al 2001, Trounce & Pinkert 2007, Bowles et al 2008, Gomez et al 2009). Xenooplasmic transfer (XOT) in somatic cell models is a valuable tool to address nuclear and mtDNA interactions between different species (Kenyon & Moraes 1997, Trounce & Pinkert 2007.…”

Section: Introductionmentioning

confidence: 99%

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

Amarnath

Choi²,

Moawad³

et al. 2011

Reproduction

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Inter-species somatic cell nuclear transfer (iSCNT) embryos usually fail to develop to the blastocyst stage and beyond due to incomplete reprogramming of donor cell. We evaluated whether using a karyoplast that would require less extensive reprogramming such as an embryonic blastomere or the meiotic spindle from metaphase II oocytes would provide additional insight into the development of iSCNT embryos. Our results showed that karyoplasts of embryonic or oocyte origin are no different from somatic cells; all iSCNT embryos, irrespective of karyoplast origin, were arrested during early development. We hypothesized that nuclear-cytoplasmic incompatibility could be another reason for failure of embryonic development from iSCNT. We used pig-mouse cytoplasmic hybrids as a model to address nuclear-cytoplasmic incompatibility in iSCNT embryos. Fertilized murine zygotes were reconstructed by fusing with porcine cytoplasts of varying cytoplasmic volumes (1/10 (small) and 1/5 (large) total volume of mouse zygote). The presence of pig cytoplasm significantly reduced the development of mouse zygotes to the blastocyst stage compared with control embryos at 120 h post-human chorionic gondotropin (41 vs 6 vs 94%, P!0.05; 1/10, 1/5, control respectively). While mitochondrial DNA copy numbers remained relatively unchanged, expression of several important genes namely Tfam, Polg, Polg2, Mfn2, Slc2a3 (Glut3), Slc2a1 (Glut1), Bcl2, Hspb1, Pou5f1 (Oct4), Nanog, Cdx2, Gata3, Tcfap2c, mt-Cox1 and mt-Cox2 was significantly reduced in cytoplasmic hybrids compared with control embryos. These results demonstrate that the presence of even a small amount of porcine cytoplasm is detrimental to murine embryo development and suggest that a range of factors are likely to contribute to the failure of inter-species nuclear transfer embryos.

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Section: Introductionmentioning

confidence: 99%

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

Amarnath

Choi²,

Moawad³

et al. 2011

Reproduction

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“…Thus, the polypeptides encoded by the nDNA must match specific sequences in the mtDNA (e.g., the displacement loop or d-loop) and the mtDNA-encoded polypeptides to enable mitochondrial function to occur normally; otherwise, the cell viability may be compromised (reviewed by Smith et al, 2005). In this regard, it is plausible to propose that the procedure of iSCNT leads to nuclear-mitochondrial incompatibilities that prevent embryo development and birth of healthy offspring (Chen et al, 2002;Gó mez et al, 2009;Hua et al, 2007;Kitiyanant et al, 2001;Lanza et al, 2000;Lu et al, 2005;Mastromonaco et al, 2007;Saikhun et al, 2002). The issue of a nuclear-mitochondrial incompatibility has been studied using xenomitochondrial cybrid models produced by the fusion of a somatic cell with a cytoplast (e.g., a cell previously enucleated) of another species (Kenyon and Moraes, 1997;Trounce and Pinkert, 2007) and using SCNT to analyze effects during embryo=fetal development in both inter- (Chen et al, 2002;Gó mez et al, 2009;Lanza et al, 2000;Loi et al, 2001;Mastromonaco et al, 2007) or intraspecific (Bowles et al, 2008;Ferreira et al, 2007;Hiendleder et al 2003Hiendleder et al , 2005Meirelles et al, 2001;Steinborn et al, 1998) animal models.…”

Section: Introductionmentioning

confidence: 99%

Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring

Chiaratti¹,

Ferreira

Meirelles³

et al. 2010

Cellular Reprogramming

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Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p ¼ 0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. On the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication.

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“…However, owing to the limited availability of oocytes from wild animals, the cloning of endangered species benefits by the use of recipient oocytes from a related domestic species. However, this methodology results in the production of nuclear-cytoplasmic hybrids [43,44]. Despite numerous attempts in a wide variety of species, the number of live births of cloned offspring is still limited to instances that combine genetic compartments of closely related species, as observed in gaur-bovine [45], mouflonsheep [46], African wild cat-domestic cat [47] and gray wolf-domestic dog experiments [48].…”

Section: Fate Of Interspecies or Intergeneric Mtdna In Ooplasmmentioning

confidence: 99%

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

Takeda

2013

Reprod Medicine & Biology

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Although somatic cell nuclear transfer (SCNT) is a powerful tool for production of cloned animals, SCNT embryos generally have low developmental competency and many abnormalities. The interaction between the donor nucleus and the enucleated ooplasm plays an important role in early embryonic development, but the underlying mechanisms that negatively impact developmental competency remain unclear. Mitochondria have a broad range of critical functions in cellular energy supply, cell signaling, and programmed cell death; thus, affect embryonic and fetal development. This review focuses on mitochondrial considerations influencing SCNT techniques in farm animals. Donor somatic cell mitochondrial DNA (mtDNA) can be transmitted through what has been considered a ''bottleneck'' in mitochondrial genetics via the SCNT maternal lineage. This indicates that donor somatic cell mitochondria have a role in the reconstructed cytoplasm. However, foreign somatic cell mitochondria may affect the early development of SCNT embryos. Nuclear-mitochondrial interactions in interspecies/intergeneric SCNT (iSCNT) result in severe problems. A major biological selective pressure exists against survival of exogenous mtDNA in iSCNT. Yet, mtDNA differences in SCNT animals did not reflect transfer of proteomic components following proteomic analysis. Further study of nuclear-cytoplasmic interactions is needed to illuminate key developmental characteristics of SCNT animals associated with mitochondrial biology.

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Cloning endangered felids using heterospecific donor oocytes and interspecies embryo transfer

Cited by 58 publications

References 37 publications

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

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