Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor

Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells

“…To the best of our knowledge, this is the first study to demonstrate gene expression of these chemerin receptors in bovine mammary gland and mammary epithelial cells. Gene expression of chemerin and GPR1 in mammary gland is thought to be derived from adipose tissue, because adipose tissue and adipocytes highly express these genes [6,8,14]. This result suggests two possible pathways in which chemerin modulates the metabolism of mammary epithelial cells; chemerin is secreted from adipocytes in the mammary gland to act in a paracrine manner or from adipose tissue of other depots and the liver to act in an endocrine manner.…”

The Regulation of Chemerin and CMKLR1 Genes Expression by TNF-慣, Adiponectin, and Chemerin Analog in Bovine Differentiated Adipocytes

“…The S-V fraction cells, which have an abundant population of preadipocytes, were isolated from the subcutaneous adipose tissues of male Japanese Black cattle and then differentiated to adipocytes as previously reported by Song et al (2010) with a modification. Briefly, confluent S-V fraction cells were incubated in DMEM/HAMF12 supplemented with 10% FBS, 1% antibiotic mix solution, octanoic acid (5.0×10 −3 M), sodium acetate (10 −2 M), lipid mixture (100-fold dilution), T 3 (2.0×10 −9 M), dexamethasone (10 −8 M), insulin (10 −7 M) and troglitazone (10 −8 M) for additional 12 d. This differentiation medium was changed every 48 h during the adipocyte differentiation.…”

“…In addition, the detailed molecular mechanism of the regulation of chemerin expression has been reported (Bauer et al, 2011; Kralisch et al, 2009; Muruganandan et al, 2011). In our previous studies, chemerin was up-regulated during bovine adipocyte differentiation and chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo (Song et al, 2010; Suzuki et al, 2012). However, the factors regulating chemerin gene expression have not been well studied in bovine adipocytes.…”

Association between a single nucleotide polymorphism in the bovine chemerin gene and carcass traits in Qinchuan cattle

“…Recently, some investigators cloned chemerin and its receptor from the adipose tissues of Japanese Black cattle, and found that the DNA sequences and amino acid sequence of bovine chemerin and its receptor were highly homologous to those of humans, mice, and pigs (Song et al, 2010). Using the RT-PCR technology, they also found that bovine chemerin mRNA was highly expressed in adipose, kidney and liver tissues than in the other histiocytes detected, such as brain, heart, lung, and so on.…”