Cloning, expression and characterization of arcelin and its impact on digestive enzymes of the stored product insect pest, Callosobruchus maculatus (F.)

Hilda, Karuppiah; Bhuvaragavan, Sreeramulu; Kamatchi, Ramakrishnan; Meenakumari, Mani; Janarthanan, Sundaram

doi:10.1016/j.pestbp.2021.104982

Cited by 8 publications

(3 citation statements)

References 59 publications

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“…For example, the banana pseudo-stem weevil, Odoiporus longicollis Olivier, larva gains the ability to resist the toxicity of stigmasterol-3-O-glucoside (SOG), sulfoquinovosyl diacylglycerol (SQDG), and betulinic acid (BA) produced by pseudo-stems through synthesizing excess α-amylase [63]. In addition, an α-amylase inhibitor extracted from Moringa oleifera leaf has insecticidal effects on Callosobruchus maculates insect larvae [64] α-Amylase inhibitors (α-AIs) have been classified by Richardson into six classes according to their tertiary structure: lectin-like, knottin-like, cereal-like, Kunitz-like, γpurothionin-like, and thaumatin-like [16]. These classes of inhibitors show significant structural diversity, resulting in different inhibition patterns and specificities against different α-amylases.…”

Section: Insect α-Amylase Inhibitorsmentioning

confidence: 99%

Insect α-Amylases and Their Application in Pest Management

Wang,

Huang,

Cao

et al. 2023

Molecules

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Amylase is an indispensable hydrolase in insect growth and development. Its varied enzymatic parameters cause insects to have strong stress resistance. Amylase gene replication is a very common phenomenon in insects, and different copies of amylase genes enable changes in its location and function. In addition, the classification, structure, and interaction between insect amylase inhibitors and amylases have also invoked the attention of researchers. Some plant-derived amylase inhibitors have inhibitory activities against insect amylases and even mammalian amylases. In recent years, an increasing number of studies have clarified the effects of pesticides on the amylase activity of target and non-target pests, which provides a theoretical basis for exploring safe and efficient pesticides, while the exact lethal mechanisms and safety in field applications remain unclear. Here, we summarize the most recent advances in insect amylase studies, including its sequence and characteristics and the regulation of amylase inhibitors (α-AIs). Importantly, the application of amylases as the nanocide trigger, RNAi, or other kinds of pesticide targets will be discussed. A comprehensive foundation will be provided for applying insect amylases to the development of new-generation insect management tools and improving the specificity, stability, and safety of pesticides.

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Section: Insect α-Amylase Inhibitorsmentioning

confidence: 99%

Insect α-Amylases and Their Application in Pest Management

Wang,

Huang,

Cao

et al. 2023

Molecules

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“…Recently specific type of protein (arcelin) was identified which were present in wild bean and shows insecticidal actions against bruchids and affect their metabolism. The alterations in their own enzymes against the arcelin shows defence mechanism of insects ( Messina et al., 2021 ; Hilda et al., 2021 ).…”

Section: Modern Pest Management Approaches ( Table 2 )mentioning

confidence: 99%

Management of stored grain pest with special reference to Callosobruchus maculatus, a major pest of cowpea: A review

Kalpna

Hajam

Kumar

2022

Heliyon

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Bruchids are most pernicious pest of stored grain pulses, especially in the tropical and subtropical areas. They penetrate into the fully grown matured pods, grains in fields and also during post-harvest storage. Among bruchids, Callosobruchus maculatus is the prominent pest having ubiquitous distribution. Chemical/synthetic insecticides provides adequate control against the C. maculatus on the pulses. However, the use of synthetic insecticides induces adverse health outcomes in agricultural workers and many causes various diseases such as cancers, genomic damage, oxidative stress, neurological disorders and respiratory, metabolic and thyroid effects. Therefore, alternative effective, safe and sustainable pest control, integration of different compatible methods should be taken into considerations. One of the possible managements might be use of traditional as well modern pest management practices. Traditional techniques include sealed containers, inert materials, harvesting time, alternate host, intercropping, storing un-threshed pulses, cleanliness, vegetable oil etc. Modern techniques such as temperature, freezing and heating, radiation treatments, resistance varieties, natural control, botanical extracts, chemical and microbial, transgenic approach, cold plasma treatments etc. thus integrated pest management might be alternative approach to combat the effect of pest. Therefore, present review aims to considers various measures for the handling of bruchids with special reference to Callosobruchus maculatus and integrated molecular inventions to decrease bruchids populations and enhance pulse productivity in pulses.

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“…In addition, against both bruchid insects, the percentage of eggs laid, hatching, adult emergence, and grain mass loss was much lower in transgenic cowpea than in control ( Grazziotin et al, 2020 ). Hilda et al (2022) demonstrated that arcelin found in the wild accession of common bean is an insecticidal protein that can prevent the bruchid beetle (Bruchidae: Coleoptera) from digesting it. Aside from focusing on coleopteran insects, arcelin was reported to be highly effective against specific insects that belong to Lepidoptera and Hemiptera insect order ( Oriani and Lara, 2000 ; Malaikozhundan et al, 2003 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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Cloning, expression and characterization of arcelin and its impact on digestive enzymes of the stored product insect pest, Callosobruchus maculatus (F.)

Cited by 8 publications

References 59 publications

Insect α-Amylases and Their Application in Pest Management

Insect α-Amylases and Their Application in Pest Management

Management of stored grain pest with special reference to Callosobruchus maculatus, a major pest of cowpea: A review

Biotechnological Approaches for Host Plant Resistance to Insect Pests

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