Cloning, Expression, and Characterization of a Type II 3-Dehydroquinate Dehydratase Gene fromStreptomyces hygroscopicus

Florova, Galina; Denoya, Claudio D.; Morgenstern, Margaret R.; Skinner, Deborah D.; Reynolds, Kevin A.

doi:10.1006/abbi.1997.0536

Cited by 10 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ansE gene product exhibits sequence similarity to type II dehydroquinate dehydratases, e.g. from Streptomyces hygroscopicus var ascomyceticus (27% identity) [28]. The third step in the AHBA biosynthetic pathway is analogous to the reaction catalyzed by a dehydroquinate dehydratase, involving a dehydration of aminodehydroquinate to aminodehydroshikimate, and is likely catalyzed by AnsE (Fig.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of ansatrienin (mycotrienin) and naphthomycin

Chen

Bamberg

Hale

et al. 1999

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

The polyketide chains of the two ansamycin antibiotics, ansatrienin (mycotrienin) and naphthomycin produced by Streptomyces collinus are assembled using 3-amino-5-hydroxybenzoic acid (AHBA) as a starter unit. The gene encoding AHBA synthase, an enzyme which catalyzes the final step of AHBA biosynthesis in the recently discovered aminoshikimate pathway, has been used to identify two separate antibiotic biosynthetic gene clusters in S. collinus. In one of these clusters, analysis of approximately 20 kb of contiguous sequence has revealed both a cluster of six genes presumed to play a role in the AHBA pathway and the beginning of a polyketide synthase (PKS) gene containing an acyl ACP ligase domain. This domain is likely responsible for loading AHBA onto the PKS. This gene cluster also contains chcA, encoding the enzyme 1-cyclohexenylcarbonyl CoA reductase, which is essential for the biosynthesis of the cyclohexanecarboxylic acid moiety of ansatrienin from shikimic acid, and a peptide synthetase. This gene cluster thus seems to control the biosynthesis of ansatrienin, which contains a side chain of N-cyclohexanecarbonyl-d-alanine esterified to the macrocyclic lactam backbone. In the putative naphthomycin biosynthetic gene cluster approximately 13 kb of contiguous sequence has revealed a second set of the genes required for AHBA biosynthesis. In addition the end of a polyketide synthase and a gene putatively involved in termination of the chain extension process, formation of an intramolecular amide bond between the AHBA nitrogen and the carboxyl group of the fully extended polyketide chain, have been identified. Thus, despite commonality in biosynthesis, the ansatrienin and naphthomycin biosynthetic gene clusters show clear organizational differences and carry separate sets of genes for AHBA biosynthesis.

show abstract

Section: Resultsmentioning

confidence: 99%

Biosynthesis of ansatrienin (mycotrienin) and naphthomycin

Chen

Bamberg

Hale

et al. 1999

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Subsequent expression of the gene in E. coli gave rise to an enzyme displaying the characteristics of a type II 3-dehydroquinase. 10,11 Interestingly, the enzyme is heptameric in nature which contrasts to most other type II 3-dehydroquinases which have been found to be dodecamers. Furthermore, the gene encoding for 3-dehydroquinase does not appear to be clustered with other shikimate pathway genes.…”

Section: -Dehydroquinasementioning

confidence: 97%

The biosynthesis of shikimate metabolites (1998)

Knaggs¹

2000

Nat. Prod. Rep.

View full text Add to dashboard Cite

“…ascomyceticus. Type-II DHQases are a family of related thermostable enzymes, that catalyze the conversion of dehydroquinic acid to dehydroshikimic acid (Figure ) . Depending upon the microorganism, a type-II DHQase is involved in the quinic acid catabolic pathway, the shikimic acid biosynthetic pathway, or both pathways. − A role of the S. hygroscopicus var.…”

mentioning

confidence: 99%