Cloning, expression and enzyme activity analysis of testicular 11β-hydroxysteroid dehydrogenase during seasonal cycle and after hCG induction in air-breathing catfish Clarias gariepinus

Rasheeda, M. K.; Kagawa, Hirohiko; Kirubagaran, R.; Dutta-Gupta, Aparna; Senthilkumaran, B.

doi:10.1016/j.jsbmb.2010.02.014

Cited by 38 publications

(24 citation statements)

References 47 publications

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“…Therefore, the extensive distribution and activity of Hsd11b2 in the adult zebrafish brain leads us to propose that persistent neurogenesis in adult zebrafish is achieved, in part, by Hsd11b2 mitigating excess glucocorticoid-mediated impact on differentiation, but this remains to be tested. Teleost peripheral tissues broadly express hsd11b2 (Jiang et al (2003), Kusakabe et al (2003) and Rasheeda et al (2010), this study). This contrasts peripheral expression in mammals and birds, where Hsd11b2 is primarily found in aldosterone target tissues such as the kidney, colon, and heart (Klusonová et al 2008, Katz et al 2010, Wyrwoll et al 2011.…”

Section: Distribution and Functional Implicationsmentioning

confidence: 51%

“…Studies have shown that hsd11b2 transcripts are broadly expressed in peripheral tissues of teleost fish (Jiang et al 2003, Kusakabe et al 2003, Rasheeda et al 2010, but its physiological role is far from clear. A local role in testicular development and gametogenesis related to 11-ketotestosterone production is the only described function to date (Kusakabe et al 2003, Rasheeda et al 2010.…”

Section: Introductionmentioning

confidence: 99%

“…A local role in testicular development and gametogenesis related to 11-ketotestosterone production is the only described function to date (Kusakabe et al 2003, Rasheeda et al 2010. Furthermore, the presence and function of Hsd11b2 in the teleost CNS has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation

Alderman

Vijayan

2012

Journal of Endocrinology

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The type 2, 11b-hydroxysteroid dehydrogenase (Hsd11b2) converts active glucocorticoids to their inactive derivatives (e.g. cortisol to cortisone). In most vertebrates, Hsd11b2 is essential for conferring aldosterone-specific actions in mineralocorticoid target tissues and for protecting glucocorticoid-sensitive tissues during stress. However, teleosts do not synthesize aldosterone, and the function of Hsd11b2 is poorly defined. The distribution of Hsd11b2 in nonmammalian brain is also largely unexplored. We tested the hypothesis that modulation of brain Hsd11b2 activity is involved in stressor-mediated cortisol regulation in zebrafish (Danio rerio). In adult zebrafish, the stress effect on Hsd11b2 expression in the brain was tested using acute air exposure followed by recovery over a 24-h period. hsd11b2 transcripts were found in nearly all peripheral tissues examined, and a spatial map of its mRNA abundance in unstressed zebrafish brain revealed extensive distribution. Stressor exposure increased the conversion of 3 H-cortisol to 3 H-cortisone indicating enhanced Hsd11b2 activity in zebrafish brain. Promoter analysis of zebrafish hsd11b2 gene revealed putative sites for cortisol-mediated transcriptional regulation of this gene. Furthermore, inhibition of Hsd11b2 activity by 18b-glycyrrhetinic acid resulted in elevated whole-body cortisol levels and preoptic area mRNA abundance of corticotropin-releasing factor and mineralocorticoid receptor. Taken together, our results underscore an important role for brain Hsd11b2 involvement in the negative feedback regulation of cortisol poststress in zebrafish.

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Section: Distribution and Functional Implicationsmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

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11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation

Alderman

Vijayan

2012

Journal of Endocrinology

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“…Both 11␤-HSD1 and 11␤-HSD2 are expressed in the adult male reproductive tract, although there are substantial species differences (4,369). In fish testis, 11␤-HSD2 plays a glucocorticoid-independent role, converting 11␤-hydroxytestosterone to 11-ketotestosterone, a major fish androgen important in fertility and sex determination (197,324,573). 11␤-HSD2 is absent from the chicken testis (351).…”

Section: The Reproductive Systemmentioning

confidence: 99%

11β-Hydroxysteroid Dehydrogenases: Intracellular Gate-Keepers of Tissue Glucocorticoid Action

Chapman

Holmes

Seckl

2013

Physiological Reviews

736

601

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Glucocorticoid action on target tissues is determined by the density of “nuclear” receptors and intracellular metabolism by the two isozymes of 11β-hydroxysteroid dehydrogenase (11β-HSD) which catalyze interconversion of active cortisol and corticosterone with inert cortisone and 11-dehydrocorticosterone. 11β-HSD type 1, a predominant reductase in most intact cells, catalyzes the regeneration of active glucocorticoids, thus amplifying cellular action. 11β-HSD1 is widely expressed in liver, adipose tissue, muscle, pancreatic islets, adult brain, inflammatory cells, and gonads. 11β-HSD1 is selectively elevated in adipose tissue in obesity where it contributes to metabolic complications. Similarly, 11β-HSD1 is elevated in the ageing brain where it exacerbates glucocorticoid-associated cognitive decline. Deficiency or selective inhibition of 11β-HSD1 improves multiple metabolic syndrome parameters in rodent models and human clinical trials and similarly improves cognitive function with ageing. The efficacy of inhibitors in human therapy remains unclear. 11β-HSD2 is a high-affinity dehydrogenase that inactivates glucocorticoids. In the distal nephron, 11β-HSD2 ensures that only aldosterone is an agonist at mineralocorticoid receptors (MR). 11β-HSD2 inhibition or genetic deficiency causes apparent mineralocorticoid excess and hypertension due to inappropriate glucocorticoid activation of renal MR. The placenta and fetus also highly express 11β-HSD2 which, by inactivating glucocorticoids, prevents premature maturation of fetal tissues and consequent developmental “programming.” The role of 11β-HSD2 as a marker of programming is being explored. The 11β-HSDs thus illuminate the emerging biology of intracrine control, afford important insights into human pathogenesis, and offer new tissue-restricted therapeutic avenues.

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“…Steroidogenic genes were found in different tissues (e.g., 11bhsd2 transcripts accumulated in tilapia testis and ovary), and these genes were also found in the testis, liver, kidney, and gill tissues of catfish (Rasheeda et al, 2010). cyp11b1 and cyp11a2 (homologs of cyp11a1 gene copies) played important roles in androgen synthesis (Parajes et al, 2013), and they are associated with sex differentiation in teleosts (Hsu et al, 2009;Evans and Nunez, 2010).…”

Section: Tissue-specific Distribution Of the Target Genesmentioning

confidence: 99%

Large-scale tissue-specific and temporal gene expression profiles in Pengze crucian carp

Zheng¹,

Chen²,

Wang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In the present study, the tissue-specific and temporal gene expression profiles of four catalogues of gonadal development-related genes (sex differentiation-related, steroid receptor, steroidogenic, and structural genes) were detected in nine tissues and during 11 successive developmental stages in the Pengze crucian carp (Pcc) (a triploid monofemale gynogenic fish). The results showed that these target genes exhibited overlapping distributions in various tissues, with the exception of Pcc-vasa and Pcc-cyp17a1. Gene expression profiling of the developmental stages showed that all of the target genes simultaneously reached peak expression levels at 40 and 48 days post hatching (dph). Both 40 and 48 dph appeared to be two key time points associated with the process of Pcc gonadal development. These data will provide a clear understanding of gene expression patterns associated with the gonadal developmentrelated genes of this gynogenic teleost.

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Cloning, expression and enzyme activity analysis of testicular 11β-hydroxysteroid dehydrogenase during seasonal cycle and after hCG induction in air-breathing catfish Clarias gariepinus

Cited by 38 publications

References 47 publications

11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation

11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation

11β-Hydroxysteroid Dehydrogenases: Intracellular Gate-Keepers of Tissue Glucocorticoid Action

Large-scale tissue-specific and temporal gene expression profiles in Pengze crucian carp

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