Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds

Subramanian, B. Mohana; Sriraman, Rajan; Rao, N. Hanumantha; Raghul, J.; Thiagarajan, D.; Srinivasan, V.A.

doi:10.1016/j.vaccine.2008.04.024

Cited by 46 publications

(16 citation statements)

References 30 publications

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“…Enhancement of host immune response was also achieved by co-administration of the chicken interleukin-2 (IL-2) gene (Ding et al2005). EtMIC1 and EtMIC2 could induce high humoral and CMI response and vaccination along with interleukin (IL)-8, IL-16, transforming growth factor-b4, or lymphotactin resulted in considerably reduction of oocyst output and increased bodyweight gain, following challenge, (Subramanian et al 2008;Lillehoj et al 2005).…”

Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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Coccidiosis is the most important protozoan disease affecting the poultry industry worldwide. Control of poultry coccidiosis is presently based on managerial skills and the use of prophylactic coccidiostatic drugs. With the emergence of drug resistant Eimeria strains, emphasis has been laid on development and use of safer vaccines; some of them have been commercialized successfully. The present review deals with the various factors responsible for the development of clinical coccidiosis in poultry as well as an overview of the currently available inducers and boosters of immunity against coccidiosis. There are three groups of vaccines currently available against coccidiosis which can be distinguished on the basis of characteristics of the Eimeria species included in the respective products, viz. vaccines based on live virulent strains, vaccines based on live attenuated strains, and vaccines based on live strains that are relatively tolerant to the ionophore compounds. The latter vaccine combines the early chemotherapeutic effect of ionophores with the late prophylactic effect of vaccination. Although in the near future more varieties of oocyst based live vaccines are expected, identification of selective coccidian-specific immunoprotective molecules is likely to get more attention to facilitate the sustainable control of poultry coccidiosis.

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Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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“…Vaccination with the recombinant Eimeria 3-1E protein could significantly affect both the cellular-mediated and mucosa-associated immune responses to live Eimeria species (Jang et al 2011). E. tenella sporozoite/merozoites antigen was originally expressed in Escherichia coli (Subramanian et al 2008;Jang et al 2010;Zhao et al 2011), but use of B. subtilis to express 3-1E protein has not been reported before. A potential advantage of B. subtilis spores as vaccine vehicles is that bacterial spores are naturally resistant to different environmental stresses, such as extremely heat and pH.…”

Section: Discussionmentioning

confidence: 99%

Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein

et al. 2015

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The current experiment was conducted to construct recombinant Bacillus subtilis WB600 expressing Eimeria tenella 3-1E protein to investigate the oral immunization protective effects against E. tenella. The merozoite surface antigen 3-1E gene of E. tenella was introduced into the pBS-H1 expression vector with a novel signal peptide sequence. After the electro-transformation, the expression of objective protein in B. subtilis WB600 was detected by Western blot. The results showed that the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Seven-day-old broiler chickens were orally vaccinated with B. subtilis WB600 harboring 3-1E (B.S-pBS-H1-3-1E) or B. subtilis WB600 with empty plasmid (B.S-pBS-H1) 10 days prior to challenge with sporulated E. tenella oocysts. The results showed the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Vaccination with B.S-pBS-H1-3-1E strain significantly increased the anti-coccidial index and reduced cecal lesion scores compared with the positive control group (chickens were challenged with sporulated E. tenella oocysts without oral administration of B.S-pBS-H1-3-1E strain) and B.S-pBS-H1 group. Ceca mucosal sIgA, secretion, and IL-2, IL-12, IFN-γ, and IL-10 level after challenge were greater in the B.S-pBS-H1-3-1E group than in the positive control group. Taken together, these results indicated that B. subtilis WB600 harboring 3-1E protein induces protective immunity against E. tenella.

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“…More and more E. tenella antigens are found to be efficient as vaccine candidates with or without specific adjuvant (Song et al 2009c;Subramanian et al 2008;Xu et al 2008;Li et al 2011;Ding et al 2008). Profilin is expressed in all sexual stages of E. tenella, stimulates cell-mediated immunity, and contains a putative conserved domain for the actin-regulatory protein profilin (Ding et al 2004;Jang et al 2010;Lee et al 2010b;Song et al 2000;Lillehoj et al 2000;Zhao et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens

Zhang

Sun

et al. 2012

Parasitol Res

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An experiment was conducted to study the adjuvant effect of ginsomes on the recombinant profilin in coccidian-infected breeding birds. Three-day-old chickens were vaccinated with Eimeria tenella recombinant profilin antigen (10, 50, and 100 μg per chicken) with or without 50 μg ginsomes per chicken. The boost vaccination was carried out 14 days later. Two weeks after the booster, the chickens were challenged with 1.5 × 10(4) homologous sporulated oocysts. The specific antibody response, lymphocyte proliferation, and IL-1 release from lymphocyte were measured at 1-42 days after boost vaccination. Seven days post-challenge, the rate of survival, body weight gains (BWG) were examined then all chickens were sacrificed and lesion scores and oocysts per gram were monitored to evaluate the protective effects of the vaccination after challenge. Compared with the group of vaccinating with profilin only, groups of 50 and 100 μg antigen plus ginsomes significantly enhanced lymphocyte proliferation and IL-1 secretion. The profilin specific antibody level in the four vaccinated groups was significantly higher than in the control group and in groups vaccinated with profilin containing ginsomes than profilin only. In the groups vaccinated with profilin plus ginsomes, the BWG was significantly higher than that of group of profilin only, but there was no significant difference between profilin plus adjuvant ginsomes, diclazuril medicated and uninfected-unmedicated-unvaccinated control groups. The lesion scores in groups immunized with profilin plus ginsomes was significantly lower than that both of groups unimmunized-challenged-unmedicated control and group vaccinated with profilin only. Oocyst excretion in groups vaccinated with 50 or 100 μg profilin plus ginsomes was lower than that of groups vaccinated with profilin only. These results demonstrate that the adjuvant ginsomes can promote subunit vaccine to induce a strong immune response and protective effects.

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Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds

Cited by 46 publications

References 30 publications

Control of poultry coccidiosis: changing trends

Control of poultry coccidiosis: changing trends

Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein

Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens

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