Cloning, expression and functional characterization of the polyunsaturated fatty acid elongase ( ELOVL5 ) gene from sea cucumber ( Apostichopus japonicus )

Wenxia, Li; Feng, Zhengfu; Song, Xiaojun; Zhu, Wei; Hu, Yanjiang

doi:10.1016/j.gene.2016.08.023

Cited by 17 publications

(10 citation statements)

References 30 publications

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“…and Paradiopatra sp., and the very low DHA content (Pairohakul 2013), it can be speculated that polychaetes possess PUFA elongases with low affinity towards C 20 or longer PUFA substrates. With regards to echinoderms, a gene annotated as "Elovl5" has been cloned and functionally characterised from the sea cucumber A. japonicus (Li et al 2016). While the deduced amino acid sequence contains all characteristic elements of the Elovl protein family, its categorisation as Elovl5 is not entirely clear from the phylogenetic analysis (Li et al 2016).…”

Section: Other Invertebratesmentioning

confidence: 99%

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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Invertebrates represent a large proportion of the biomass in aquatic ecosystems, particularly in the ocean. In recent years, invertebrates have been well-consolidated models in evolutionary developmental biology research and, consequently, genomic and transcriptomic sequence databases from a plethora of species across the animal kingdom have become available. This has provided an excellent source of evidence confirming that invertebrates operate endogenous mechanisms for PUFA production. The present paper reviews the current knowledge of gene complement and, where possible, the function of desaturases and elongases with pivotal roles in PUFA biosynthesis of aquatic invertebrates. More specifically, this review covers three major enzyme types, namely ωx desaturases, front-end desaturases and elongases, that have been characterised from species of sponges, cnidarians, molluscs, annelids, crustaceans, rotifers, echinoderms and non-vertebrate chordates (amphioxus and sea squirt). These studies have shown that invertebrates operate alternative and unusual pathways of PUFA biosynthesis involving gene families with complex phylogeny and functional diversity. Consequently, research in this area provides potentially valuable molecular tools in the form of genes that can be used in the biotechnological production of n-3 products.

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Section: Other Invertebratesmentioning

confidence: 99%

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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“…In contrast to freshwater fish, the conversion efficiency of either ALA or LA to LC‐PUFA in marine fish is lower, although LC‐PUFA is required for their survival and growth (Ruyter et al, ). Some invertebrates such as Haliotis discus hannai , Haliotis fulgens , and Apostichopus japonicus have been reported to have the capacity to synthesize EPA and DHA from ALA using elongation and desaturation (Li, Feng, Song, Zhu, & Hu, ; Liu, Wang, Feng, Song, & Zhu, ; Mateos, Lewandowski, & Su, ).…”

Section: Introductionmentioning

confidence: 99%

“…The sea cucumber A. japonicus , a representative echinoderm species, has great aquaculture potential in China and is well recognized as a good food for human health (Anderson, Flemming, Watson, & Lotze, ). Previous studies suggested that it has the capacity to synthesize EPA and DHA from either ALA or LA through elongation and desaturation (Li et al, ; Liu et al, ). However, there has been no detailed study of either the capacity for endogenous PUFA biosynthesis or the nutritional regulation of PUFA biosynthesis in sea cucumber to date.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary n‐3 PUFA supplements on composition of n‐3 PUFA and expression of fatty acid elongase 5 (AJELOVL5) in sea cucumber, Apostichopus japonicus

Zhao

Feng

et al. 2018

Aquac Res

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This is the first comprehensive study on the effect of dietary polyunsaturated fatty acid (PUFA) levels on the expression of fatty acid elongase 5 (AJELOVL5), PUFA composition, and growth in juvenile sea cucumbers. The specific growth rate (SGR w ) was improved in n-3 PUFA-rich diets compared to low n-3 PUFA diets. AJELOVL5 expression was apparently upregulated in juveniles fed lower PUFA diets relative to higher PUFA diets, with higher expression in the body wall and respiratory tree of juveniles fed diets without ɑ-linolenic acid (ALA, 18:3n-3) compared to juveniles fed higher ALA level diets; similar results were also detected in juveniles fed diets with lower eicosapentaenoic acid (EPA, 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and none of ALA, EPA, or DHA respectively. The concentrations of ALA, EPA, and DHA in tissues were positively related to the content of dietary corresponding PUFA, with higher ALA content in juveniles fed diet ALA 12.71 than in the ALA 7.46 and ALA 0 groups. Similar results were also obtained in sea cucumber fed diets enriched with either EPA or DHA. Interestingly, considerable levels of EPA and DHA were found in the tissues of juveniles fed diets of CK 0 and DHA 0 , with no specific input of EPA or DHA, showing that the sea cucumber was capable of biosynthesizing EPA and DHA from their corresponding precursors as ALA and linoleic acid (LA,.

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“…The Elovl protein family consists of seven members (Elovl 1–7) among which Elovl2, Elovl4 and Elovl5 are generally regarded as the critical enzymes in the elongation of LC‐PUFA. Functional characterization of isolated Elovl enzymes has also been performed using the yeast expression system in various fish species (Agaba, Tocher, Dickson, Dick, & Teale, ; Leonard, Pereira, Sprecher, & Huang, ; Li, Feng, Song, Zhu, & Hu, ). These studies suggest that most Elovl5 enzymes prefer to add two carbons to the C18 and C20 fatty acids (Agaba et al, , ; Gregory & James, ; Gregory et al, ; Hastings et al, ; Kuah, Jaya‐Ram, & Shu‐Chien, ; Mohd‐Yusof, Monroig, Mohd‐Adnan, Wan, & Tocher, ; Monroig, Wang, et al, ; Morais, Monroig, Zheng, Leaver, & Tocher, ; Morais et al, ; Wang et al, ; Zheng et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…These studies suggest that most Elovl5 enzymes prefer to add two carbons to the C18 and C20 fatty acids (Agaba et al, , ; Gregory & James, ; Gregory et al, ; Hastings et al, ; Kuah, Jaya‐Ram, & Shu‐Chien, ; Mohd‐Yusof, Monroig, Mohd‐Adnan, Wan, & Tocher, ; Monroig, Wang, et al, ; Morais, Monroig, Zheng, Leaver, & Tocher, ; Morais et al, ; Wang et al, ; Zheng et al, ). Elovl4 is more efficient in the elongation of C20 and C22 fatty acids (Castro et al, ; Guillou et al, ; Jakobsson et al, ; Li et al, ), and Elovl2 is preferentially responsible for the elongation step from C22 to C24 fatty acids (Kim et al, ; Morais et al, ; Xie et al, ).…”

Section: Introductionmentioning

confidence: 99%

Cloning, characterization and transcription analysis of fatty acyl desaturase ( Δ6Fad‐like ) and elongase ( Elovl4‐like , Elove5‐like ) during embryos development of Strongylocentrotus intermedius

et al. 2019

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Strongylocentrotus intermedius has high nutritional value because it is rich in proteins, amino acids and long‐chain polyunsaturated fatty acids (LC‐PUFA). LC‐PUFA are essential nutrients that not only determine the nutritional value of sea urchins but also guarantee normal growth and reproduction performance. To better understand the molecular basis of LC‐PUFA biosynthesis in S. intermedius, the Δ6Fad‐like, Elovl4‐like and Elovl5‐like genes were cloned and fatty acid compositions during the early developmental stages of sea urchins were detected. The full‐length of Δ6Fad‐like was 2,199 bp, with a putative open reading frame of 1,248 bp encoding a polypeptide of 415 amino acid (AA). The Elovl4‐like and Elovl5‐like genes encoded 310 and 234 AA, respectively, which exhibited all of the characteristics of the Elovl family, such as a histidine box motif and putative transmembrane‐spanning domains. Tissue distribution analysis revealed that Δ6Fad‐like, Elovl4‐like and Elovl5‐like genes were expressed at the highest levels in the gonads and intestine, and the expression levels gradually increased in embryos during development. These results can help to understand the role of the Δ6Fad‐like, Elovl4‐like and Elovl5‐like genes in the different developmental stages of the sea urchin and to clarify the biosynthetic pathways of LC‐PUFA during the development of the sea urchin and provide a theoretical basis for improving the quality and breeding of excellent traits in sea urchins.

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Cloning, expression and functional characterization of the polyunsaturated fatty acid elongase ( ELOVL5 ) gene from sea cucumber ( Apostichopus japonicus )

Cited by 17 publications

References 30 publications

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

Effects of dietary n‐3 PUFA supplements on composition of n‐3 PUFA and expression of fatty acid elongase 5 (AJELOVL5) in sea cucumber, Apostichopus japonicus

Cloning, characterization and transcription analysis of fatty acyl desaturase ( Δ6Fad‐like ) and elongase ( Elovl4‐like , Elove5‐like ) during embryos development of Strongylocentrotus intermedius

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