Cloning, Expression and Genomic Organization of Genes Encoding Major Royal Jelly Protein 1 and 2 of the Honey Bee (Apis cerana)

Imjongjirak, Chanprapa; Klinbunga, Sirawut; Sittipraneed, Siriporn

doi:10.5483/bmbrep.2005.38.1.049

Cited by 25 publications

(21 citation statements)

References 21 publications

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“…In addition to A. mellifera, the genomes of three related honey bee species-Apis cerana, Apis dorsata, and Apis florea-have genes encoding MRJPs (Albertova et al 2005;Imjongjirak et al 2005;Su et al 2005). These data support the following two conclusions: The mrjp duplication events were recent, and the distribution of mrjp genes within animals is very restricted.…”

Section: Drapeau Et Alsupporting

confidence: 60%

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees

Drapeau¹,

Albert²,

et al. 2006

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The genomic architecture underlying the evolution of insect social behavior is largely a mystery. Eusociality, defined by overlapping generations, parental brood care, and reproductive division of labor, has most commonly evolved in the Hymenopteran insects, including the honey bee Apis mellifera. In this species, the Major Royal Jelly Protein (MRJP) family is required for all major aspects of eusocial behavior. Here, using data obtained from the A. mellifera genome sequencing project, we demonstrate that the MRJP family is encoded by nine genes arranged in an ∼60-kb tandem array. Furthermore, the MRJP protein family appears to have evolved from a single progenitor gene that encodes a member of the ancient Yellow protein family. Five genes encoding Yellow-family proteins flank the genomic region containing the genes encoding MRJPs. We describe the molecular evolution of these protein families. We then characterize developmental-stage-specific, sex-specific, and caste-specific expression patterns of the mrjp and yellow genes in the honey bee. We review empirical evidence concerning the functions of Yellow proteins in fruit flies and social ants, in order to shed light on the roles of both Yellow and MRJP proteins in A. mellifera. In total, the available evidence suggests that Yellows and MRJPs are multifunctional proteins with diverse, context-dependent physiological and developmental roles. However, many members of the Yellow/MRJP family act as facilitators of reproductive maturation. Finally, it appears that MRJP protein subfamily evolution from the Yellow protein family may have coincided with the evolution of honey bee eusociality.

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Section: Drapeau Et Alsupporting

confidence: 60%

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees

Drapeau¹,

Albert²,

et al. 2006

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“…In addition to these obvious advantages in using insect cell lines for solving the CCD issue, we and others have demonstrated that production of insect venom allergens in insect cells is generally superior in terms of functionality, epitope authenticity and folding . That this also holds true for MRJPs is underlined by the only other report concerning the recombinant production of MRJPs, MRJP1 and MRJP2 from Apis cerana , which could be produced in Escherichia coli only in insoluble form .…”

Section: Discussionmentioning

confidence: 95%

The major royal jelly proteins 8 and 9 (Api m 11) are glycosylated components of Apis mellifera venom with allergenic potential beyond carbohydrate‐based reactivity

Blank

Bantleon

McIntyre

et al. 2012

Clin Experimental Allergy

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The glycosylated MRJP8 and MRJP9 of HBV have IgE-sensitizing potential in HBV-allergic patients beyond CCD reactivity and have to be considered as allergens, which might be potentially important for a fraction of venom allergic patients. They are valuable tools to elucidate individual component-resolved reactivity profiles of venom allergic patients and to provide insights into the role of particular venom components. Due to their allergenic properties, MRJP8 and MRJP9 were designated as isoallergens Api m 11.0101 and Api m 11.0201 respectively.

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“…The Yellow gene family from which MRJPs are derived (pfam03022) is widespread within the Arthropoda, but MRJPs are currently unknown beyond the genus Apis . It is noteworthy that A. mellifera , A. cerana , A. dorsata and A. florea are all highly eusocial (Michener, 1974), and all have genes encoding MRJPs (Albertova et al ., 2005;Imjongjirak et al ., 2005;Su et al ., 2005). Whether the phylogenetic association between sociality and MRJPs is significant has not yet been tested, but the apparent coevolution between the two characters suggests that certain MRJPs evolved to help signal or regulate the expression of social traits, possibly including worker sterility.…”

Section: Discussionmentioning

confidence: 99%

Towards a molecular definition of worker sterility: differential gene expression and reproductive plasticity in honey bees

Thompson

Kucharski

Maleszka

et al. 2006

Insect Molecular Biology

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We show that differences in the reproductive development of honey bee workers are associated with locusspecific changes to abundance of messenger RNA. Using a cross-fostering field experiment to control for differences related to age and environment, we compared the gene expression profiles of functionally sterile workers (wild-type) and those from a mutant strain in which workers are reproductively active (anarchist). Among the set of three genes that are significantly differentially expressed are two major royal jelly proteins that are up-regulated in wild-type heads. This discovery is consistent with sterile workers synthesizing royal jelly as food for developing brood. Likewise, the relative underexpression of these two royal jellies in anarchist workers is consistent with these workers' characteristic avoidance of alloparental behaviour, in favour of selfish egg-laying. Overall, there is a trend for the most differentially expressed genes to be up-regulated in wild-type workers. This pattern suggests that functional sterility in honey bee workers may generally involve the expression of a suite of genes that effectively 'switch' ovaries off, and that selfish reproduction in honey bee workers, though rare, is the default developmental pathway that results when ovary activation is not suppressed.

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Cloning, Expression and Genomic Organization of Genes Encoding Major Royal Jelly Protein 1 and 2 of the Honey Bee (Apis cerana)

Abstract: Major Royal Jelly Protein cDNAs of

Cited by 25 publications

References 21 publications

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees

The major royal jelly proteins 8 and 9 (Api m 11) are glycosylated components of Apis mellifera venom with allergenic potential beyond carbohydrate‐based reactivity

Towards a molecular definition of worker sterility: differential gene expression and reproductive plasticity in honey bees

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