Cloning, expression, and preliminary characterization of the dysferlin tegument protein in Schistosoma japonicum

Xiong, Yanian; Ai, Dezhou; Meng, Peipei; Wei, Meimei; Hong, Yang; Zhang, Min; Huang, Lini; Fu, Zhiqiang; Shi, Yiting; Lin, Jiaojiao

doi:10.1016/j.parint.2013.07.008

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…This assumption is supported by the observed down-regulation of tegument-associated genes (69,70), such as the components of basement membrane including type I, IV collagens (Sjp_0034120, Sjp_0099760) and a basement membrane-specific heparin sulfate proteoglycan core protein (Sjp_0073100), the cytoskeleton and motor proteins, including keratin 9 (Sjp_ 0075140), dynein light chain 2 (Sjp_0008620) and paramyosin (Sjp_0027340), and tegument surface protein dysferlin (Sjp_0078120) and myoferlin (Sjp_0090660) of males, as well as the cell adhesion protein C-type lectin (Sjp_0033720) of females. Some of these were possibly targeted by praziquantel, causing tegument damage (68)(69)(70)(71). In addition to their roles in morphology maintenance, orthologs of these genes may also be involved in worm survival, egg production (72)(73)(74), and embryonic development (75), as well as in vitelline development and maturation (40,66).…”

Section: Discussionmentioning

confidence: 99%

Serine/threonine protein phosphatase 1 (PP1) controls growth and reproduction in Schistosoma japonicum

Zhao

et al. 2018

The FASEB Journal

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Schistosomiasis is a human parasitic disease caused by flatworms of the genus Schistosoma. Adult female schistosomes produce numerous eggs that are responsible for the pathogenesis and transmission of the disease, and the maturation of female gonads depends on the permanent pairing of females and males. Signaling protein kinases have been proven to control female gonad differentiation after pairing; however, little is known about the roles of protein phosphatases in the developmental and reproductive biology of schistosomes. Here we explored 3 genes encoding catalytic subunits of serine/threonine protein phosphatase 1 (PP1c) that were structurally and evolutionarily conserved in Schistosoma japonicum. In situ hybridization showed transcripts of 3 Sj-pp1c genes mainly localized in the reproductive organs and tissues. Triple knockdown of Sj-pp1c genes by RNA interference caused stunted growth and decreased pairing stability of worm pairs, as well as a remarkable reduction in cell proliferation activity and defects in reproductive maturation and fecundity. Transcriptomic analysis post-RNA interference suggested that Sj-pp1c genes are involved in controlling worm development and maturation mainly by regulating cell proliferation, eggshell synthesis, nutritional metabolism, cytoskeleton organization, and neural process. Our study provides the first insight into the fundamental contribution of Sj-PP1c to molecular mechanisms underlying the reproductive biology of schistosomes.-Zhao, L., Lu, Z., He, X., Mughal, M. N., Fang, R., Zhou, Y., Zhao, J., Gasser, R. B., Grevelding, C. G., Ye, Q., Hu, M. Serine/threonine protein phosphatase 1 (PP1) controls growth and reproduction in Schistosoma japonicum.

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Section: Discussionmentioning

confidence: 99%

Serine/threonine protein phosphatase 1 (PP1) controls growth and reproduction in Schistosoma japonicum

Zhao

et al. 2018

The FASEB Journal

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“…Moreover, a protein-protein interaction network was constructed to identify proteins that potentially interact with SjANX A13. Among the identified proteins, expression of UDP-glucose 4-epimerase [ 64 ] and dysferlin [ 65 ] was mainly limited to the tegument cells of S. japonicum and a recombinant protein of UDP-glucose 4-epimerase and dysferlin showed potential as a vaccine target. Besides, T-complex protein 1 is involved cytoskeletal proteostasis [ 66 ] and nucleolysin TIA-1 is critical to the stress response and neurodevelopment in mammals [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and functional characterization of Schistosoma japonicum annexin A13

Zhong,

Hou,

Qin

et al. 2023

Vet Res

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Schistosomiasis is a neglected tropical disease that affects humans and animals in tropical and subtropical regions worldwide. Schistosome eggs are responsible for the pathogenesis and transmission of schistosomiasis, thus reducing egg production is vital for prevention and control of schistosomiasis. However, the mechanisms underlying schistosome reproduction remain unclear. Annexin proteins (ANXs) are involved in the physiological and pathological functions of schistosomes, but the specific regulatory mechanisms and roles of ANX A13 in the development of Schistosoma japonicum and host–parasite interactions remain poorly understood. Therefore, in this study, the expression profiles of SjANX A13 at different life cycle stages of S. japonicum were assessed using quantitative PCR. In addition, the expression profiles of the homolog in S. mansoni were analyzed in reference to public datasets. The results of RNA interference showed that knockdown of SjANX A13 significantly affected the development and egg production of female worms in vivo. The results of an immune protection assay showed that recombinant SjANX A13 increased production of immunoglobulin G-specific antibodies. Finally, co-culture of S. japonicum exosomes with LX-2 cells using a transwell system demonstrated that SjANX A13 is involved in host–parasite interactions via exosomes. Collectively, these results will help to clarify the roles of SjANX A13 in the development of S. japonicum and host–parasite interactions as a potential vaccine candidate.

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“…The latter have been identified as part of protein complexes known as tetraspanin-enriched microdomains that mediate a range of processes at the surface of the plasma membrane (Jia et al 2014;Schulte et al 2013). In addition, calpain was shown to modify the worm's local environment by cleaving the host clotting protein fibronectin and the coagulation protein high molecular weight kininogen to ensure parasite survival, and dysferlin is reported to be involved in membrane repair mechanisms (Wang et al 2018;Xiong et al 2013). In contrast, the expression of enolase was only increased in male_single after 0.5 h in NHS.…”

Section: Discussionmentioning

confidence: 99%

Human serum activates the tegument of female schistosomes and supports recovery from Praziquantel

et al. 2020

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Schistosomiasis is one of the most devastating parasitic disease in the world. Schistosoma spp. survive for decades within the vasculature of their human hosts. They have evolved a vast array of mechanisms to avoid the immune reaction of the host. Due to their sexual dimorphism, with the female worm lying within the gynecophoric canal of the male worm, it is the male that is exposed to the immediate environment and the soluble parts of the host’s immune response. To understand how the worms are so successful in fending off the immune attacks of the host, comparative analyses of both worm sexes in human serum (with or without Praziquantel) were performed using scanning electron microscopy, transmission electron microscopy, and immunohistochemistry. Further, gene expression analyses of tegument-specific genes were performed. Following the incubation in human serum, males and females out of pairs show morphological changes such as an altered structure of the pits below the surface and an increased number of pits per area. In addition, female schistosomes presented a marked tuft-like repulsion of their opsonized surface. The observed resistance of females to Praziquantel seemed to depend on active proteins in the human serum. Moreover, different expression profiles of tegument-specific genes indicate different functions of female_single and male_single teguments in response to human serum. Our results indicate that female schistosomes developed different evasion strategies toward the host’s immune system in comparison to males that might lead to more robustness and has to be taken into account for the development of new anti-schistosomal drugs.

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Cloning, expression, and preliminary characterization of the dysferlin tegument protein in Schistosoma japonicum

Cited by 5 publications

References 36 publications

Serine/threonine protein phosphatase 1 (PP1) controls growth and reproduction in Schistosoma japonicum

Serine/threonine protein phosphatase 1 (PP1) controls growth and reproduction in Schistosoma japonicum

Molecular and functional characterization of Schistosoma japonicum annexin A13

Human serum activates the tegument of female schistosomes and supports recovery from Praziquantel

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