Cloning, expression and purification of tRNAPro from bacteria Enterococcus faecalis

Abstract

Aim. To elaborate the method of expression and purification of bacteria Enterococcus faecalis tRNAPro transcript. Methods. tRNA, co-expressed in vitro with cis-hydrolytical ribozyme, was purified by high performance liquid chromatography using anion-exchange chromatographic column. Results. A satisfactory yield of high purity preparation was obtained. A transcript of tRNAPro exhibits acceptor activity in aminoacylation reaction. Conclusions. The method developed may be introduced in laboratory practice including the obtaining of other tRNAs

Role of tRNAPro in pretransfer editing of alanine by prolyl-tRNA synthetase

“…Ïðåïàðàòèâíîå âûäåëåíèå ÏðîÐÑEf, åå ìóòàíòíûõ ôîðì, à òàêaeå íàòèâíîé òÐÍÊ Ïðî Rhodopseudomonas palustris ñ àíòèêîäîíîì CGG (òÐÍÊ Ïðî Rp) îïèñàíî íàìè ðàíåå [18]. Ïåïàðàòû òðàíñêðèïòà òÐÍÊ Ïðî Ef ñ àíòèêîäîíîì UGG ãîòîâèëè, êàê îïèñàíî â [19].…”