CLONING, EXPRESSION PATTERNS, AND PRELIMINARY CHARACTERIZATION OF <i>AccCPR24</i>, A NOVEL RR‐1 TYPE CUTICLE PROTEIN GENE FROM <i>Apis cerana cerana</i>

Chu, Xiaoqian; Lu, Wenjing; Zhang, Yuanying; Guo, Xingqi; Sun, Rujiang; Xu, Baohua

doi:10.1002/arch.21132

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Willis () described 12 cuticular protein families based on differences in conserved motifs. Most cuticular protein genes are highly expressed in the period of molting and are regulated by molting hormones such as juvenile hormones and ecdysteroids, highlighting the importance of cuticular proteins during insect development (Riddiford et al ., ; Togawa et al ., ; Chu et al ., ). The majority of structural cuticular proteins belong to CPR family subclasses RR‐1 and RR‐2, which are based on the conserved Rebers and Riddiford (R&R) Consensus region (Ioannidou et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Cuticular protein LmTwdl1 is involved in molt development of the migratory locust

et al. 2016

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The cuticle, an essential structure for insects, is produced from cuticular proteins and chitin via a series of biochemical reactions. Tweedle genes are important members of the cuticular protein family and have four conserved motifs binding to chitin. Tweedle family genes have been found to play a profound effect on cuticle development. Here, we report that the cuticular protein gene LmTwdl1 of Locusta migratoria belongs to the Tweedle family. In situ hybridization showed that LmTwdl1 is localized to epidermal cells of the cuticle. The expression patterns of LmTwdl1 showed low expression in the cuticle during the early and middle stages of the fifth-instar nymphs; in contrast, its expression rapidly increased in the late stages of fifth-instar nymphs. We performed RNA interference to examine the function of LmTwdl1 in locusts. Silencing of LmTwdl1 resulted in high mortality during the molting process before the next stage. Also, the epicuticle of nymphs failed to molt, tended to be thinner and the arrangement of chitin in the procuticle appeared to be disordered compare to the control group. These results demonstrate that LmTwdl1 plays a critical role in molting, which contributes to a better understanding of the distinct functions of the Tweedle family in locusts.

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Section: Introductionmentioning

confidence: 97%

Cuticular protein LmTwdl1 is involved in molt development of the migratory locust

et al. 2016

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“…After RNAi of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 , qRT-PCR was used to detect the mRNA levels of AccANT , AccCDK5r , AccCPR24 , AccERR , AccTP1 , AccCDK5 , AccCYP336A1 , AccSp10 , AccRBM11 , AccAK , AccMTNR1A , and AccAIF3 . These genes were proved to be involved in the stress response in our previous studies ( Chu et al, 2013 ; Liu X. et al, 2014 ; Wang et al, 2014 ; Chen et al, 2015 ; Li et al, 2016b , 2018a ; Zhang et al, 2016 ; Zhou et al, 2016 ; Zhu et al, 2016 ; Gao et al, 2017 ; Zhao et al, 2018 ).…”

Section: Methodsmentioning

confidence: 79%

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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DnaJ, also known as Hsp40, plays important roles in maintaining the normal physiological state of an organism under stress conditions by mediating essential processes, such as protein synthesis, degradation, folding and metabolism. However, the exact functions of most DnaJ members are not fully understood in insects. Here, we identified three genes, AccDnaJA1, AccDnaJB12, and AccDnaJC8, in Apis cerana cerana and explored their connection with the environmental stress response. Quantitative real-time PCR results showed that the mRNA levels of AccDnaJA1, AccDnaJB12, and AccDnaJC8 were all induced under cold, UV, H2O2 and different pesticides treatment. The expression patterns of AccDnaJB12 and AccDnaJC8 were upregulated by CdCl2 and HgCl2 stress, while the transcriptional levels of AccDnaJA1 were downregulated by CdCl2 and HgCl2 stress. Western blot findings further indicated that AccDnaJB12 protein levels were increased by some stress conditions. Knockdown of each of these three genes downregulated the transcriptional patterns of several stress response-related genes at different levels. Functional analysis further demonstrated that the resistance of A. cerana cerana to lambda-cyhalothrin stress was reduced with knockdown of AccDnaJA1, AccDnaJB12, or AccDnaJC8, indicating that these three genes may be involved in the tolerance to this pesticide. Taken together, these findings indicate that AccDnaJA1, AccDnaJB12, and AccDnaJC8 may play pivotal roles in the stress response by facilitating honeybee survival under some adverse circumstances. To our knowledge, this is the first report that reveals the roles of DnaJ family proteins under different adverse circumstances in A. cerana cerana.

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“…The insect cuticle is the first line of defense against external chemicals, specifically the proteinaceous and chitinous procuticle (Vannini et al ., 2014). Cuticular proteins (CPs) play an important role in insect development and defense (Chu et al ., 2013). Reduced cuticular permeability is one of the key mechanisms of insect resistance (Hemingway & Karunaratne, 1998).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of thiamethoxam susceptible and resistant Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), using RNA‐sequencing

et al. 2021

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Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), transmits the causal pathogen of huanglongbing and is a global pest of citrus. D. citri populations exhibit resistance to multiple insecticide modes of action in areas where these chemicals have been overused. We performed genome‐wide transcriptional analysis for two field populations of D. citri (Wauchula and Lake Alfred, Florida, USA) that exhibit 1300‐fold resistance to the neonicotinoid insecticide, thiamethoxam, and compared it to that of susceptible psyllids collected from the same area and without imposed selection. The Lake Alfred population responded to insecticide resistance by up‐regulation of 240 genes and down‐regulation of 148 others. The Wauchula population exhibited similar patterns to the Lake Alfred population with up‐regulation of 253 genes and down‐regulation of 115 others. Gene Ontology annotation associated with cellular processes, cell, and catalytic activity were assigned to differentially expressed genes (DEGs). The DEGs from Lake Alfred and Wauchula populations were mapped to Kyoto Encyclopedia of Gene and Genomes pathways and implicated enrichment of metabolic pathways, oxidative phosphorylation, extracellular matrix‐receptor interaction, terpenoid backbone biosynthesis, and insect hormone biosynthesis in the resistant populations. Up‐regulation of 60s ribosomal proteins, UDP‐gluscoyltransferases, cytochrome c oxidases, and CYP and ABC transporters among thiamethoxam‐resistant D. citri implicates a broad array of novel and conventionally understood resistance mechanisms.

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CLONING, EXPRESSION PATTERNS, AND PRELIMINARY CHARACTERIZATION OF AccCPR24, A NOVEL RR‐1 TYPE CUTICLE PROTEIN GENE FROM Apis cerana cerana

Cited by 9 publications

References 35 publications

Cuticular protein LmTwdl1 is involved in molt development of the migratory locust

Cuticular protein LmTwdl1 is involved in molt development of the migratory locust

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Comparative transcriptome analysis of thiamethoxam susceptible and resistant Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), using RNA‐sequencing

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