Cloning, expression, purification and application of a novel chitinase from a thermophilic marine bacterium Paenibacillus barengoltzii

Purification and biochemical characterization of novel acidic chitinase from Paenicibacillus barengoltzii

“…2), which is obviously lower than those of the two chitinases (PbChi70, 70.6 kDa; PbChi74, 74 kDa) from the same strain reported in our previous studies [4,5]. [31]).…”

“…Two chitinases from marine bacterium Paenibacillus barengoltzii have been reported in our recent studies: PbChi70 is an endo type chitinase with a molecular mass of 70.6 kDa. It was most active at pH 5.5 and 55 °C, respectively, and suitable for (GlcNAc)2 production [4]. PbChi74 is an exochitinase with molecular mass of 74 kDa.…”

“…Paenicibacillus barengoltzii CAU904 is a thermophilic marine bacterium which has been reported to produce different types of chitinases in our previous studies, including an endochitinase suitable for (GlcNAc)2 production [4] and an acidic and thermostable exochitinase (PbChi74) suitable for GlcNAc production [5]. Here, we report on production, purification and characterization of another novel acidic endochitinase from Paenicibacillus barengoltzii.…”

“…Chitin, a type of abundant naturally occurring carbohydrate polymers, consists of N-acetyl-D-glucosamine (GlcNAc) units linked by β- (1,4) glycoside bonds [1]. Chitins are mainly dispersed in outer shell of crustaceans and insect cuticles, and fungal cell walls, composing one of the largest forms of renewable biomass on earth next to cellulose [2].…”

“…They have received a great deal of attention in recent years due to their biotechnological applications in different fields [3]. They can be used in the production of N-acetyl GOSs [4] and GlcNAc [5] in food or pharmaceutical industry [2], bioconversion of chitin materials to ethanol in energy industry [6], production of single cell protein in feed industry [7] and bio-controlling of fungal phytopathogens in agriculture [8,9].…”