Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium<i>Pseudoalteromonas</i>sp. AN178

Wang, Quanfu; Hou, Yanhua; Shi, Yonglei; Han, Xiao; Chen, Qian; Zhi-guo, HU; Liu, Yuanping; Li, YuJin

doi:10.1155/2014/246871

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…Cold-active microbial enzymes have attracted increasing attention in recent years ( Wang et al, 2012 ). In addition to the properties related to their structural characteristics, one of the main advantages related to the use of these enzymes is the decrease energy expenditure and processing costs associated with industrial heating steps ( Duarte et al, 2013 ).…”

Section: Biotechnological Potentialmentioning

confidence: 99%

Marine-derived fungi: diversity of enzymes and biotechnological applications

et al. 2015

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The ocean is considered to be a great reservoir of biodiversity. Microbial communities in marine environments are ecologically relevant as intermediaries of energy, and play an important role in nutrient regeneration cycles as decomposers of dead and decaying organic matter. In this sense, marine-derived fungi can be considered as a source of enzymes of industrial and/or environmental interest. Fungal strains isolated from different substrates, such as invertebrates, decaying wood, seawater, sediments, and mangrove detritus, have been reported to be producers of hydrolytic and/or oxidative enzymes, with alginate lyase, amylase, cellulase, chitinase, glucosidase, inulinase, keratinase, ligninase, lipase, nuclease, phytase, protease, and xylanase being among the enzymes produced by fungi of marine origin. These enzymes present temperature and pH optima ranging from 35 to 70∘C, and 3.0 to 11.0, respectively. High-level production in bioreactors is mainly performed using submerged-state fermentation. Certain marine-derived fungal strains present enzymes with alkaline and cold-activity characteristics, and salinity is considered an important condition in screening and production processes. The adaptability of marine-derived fungi to oceanic conditions can be considered an attractive point in the field of fungal marine biotechnology. In this review, we focus on the advances in discovering enzymes from marine-derived fungi and their biotechnological relevance.

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Section: Biotechnological Potentialmentioning

confidence: 99%

Marine-derived fungi: diversity of enzymes and biotechnological applications

et al. 2015

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“…These results were similar to Grxs from C. sorokiniana, 21 potato, 22 and Pseudoalteromonas. 23 It was previously reported that Populus trichocarpa thioredoxin (Trx h4) could be the substrate of E. coli Grxs. 12 To test whether it is the case for wheat Grx and Trx and to develop a synergetic system using food source Grx and Trx as enzymatic additives in food processing, the reduction assay of wTrx by wGrx was initially carried out.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Crystal Structure of Wheat Glutaredoxin and Its Application in Improving the Processing Quality of Flour

Sun

Chen

Feng

et al. 2018

J. Agric. Food Chem.

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Glutaredoxin (Grx) is a ubiquitous oxidoreductase that plays a vital role in maintaining cellular redox homeostasis. In comparison to Grx from other organisms, plant Grx is unique in that it has many isoforms, which, thus, suggests probably diverse functions and mechanisms. Therefore, structure−function characterization of plant Grx is necessary to have indepth knowledge and explore its application in industry. In this study, wheat Grx (wGrx) was overexpressed and purified and the crystal structure of wGrx was determined at 2.94 Å resolution. Interestingly, the structure for the first time captured both the oxidized form and the transient state of reduced−oxidized wGrx in a crystal. The mutagenesis of wGrx suggests that it adopts a monothiol catalytic mechanism. wGrx has the ability to reduce wheat thioredoxin (wTrx), and this is the first example of the reduction of thioredoxin subgroup h class II by Grx. Flour farinograph and dynamic rheological analysis showed that wGrx together with wTrx has a positive effect on dough formation, which is probably attributed to the increased sodium dodecyl sulfate (SDS)-insoluble gluten macropolymer (GMP) through increasing the intermolecular disulfide bond induced by the wGrx−wTrx system. The results indicate great potential of wGrx−wTrx as a novel synergetic enzymatic additive and may be employed to fine-tune the processing performance of food related to the redox reaction.

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“…Cold-active microbial enzymes have attracted increasing attention in recent years (Wang et al, 2012) since it is advantageous to use the cold-active enzymes owing to the reduced energy expenditure and processing costs associated with industrial heating steps besides the properties related to their structural characteristics (Duarte et al, 2013). Lowtemperature active endoglucanases are obtained from several fungal strains inhabiting the marine sponge Haliclona simulans in Ireland (Baker et al, 2010), and cold-active xylanase is produced by a marine-derived Cladosporium sp.…”

Section: Cold-active Microbial Enzymesmentioning

confidence: 99%

Bioprospecting Marine Fungal Enzymes-Scope and Challenges

Chandrasekaran¹,

Kalaiselvam²

2023

Kavaka

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Marine microorganisms are promising source of enzymes with industrial applications due to their immense genetic and biochemical diversity. Availability of novel enzymes, advancements in enzymology and enzyme technology have significantly contributed to the industrial application of enzymes and the rapid expansion of the enzyme market. In this context marine fungal enzymes assume greater attention recently owing to the great demand for novel and efficient biocatalysts for industrial applications and other services. This situation has warranted exploration of marine fungal biodiversity for new enzymes. The present review focus on bioprospecting of marine fungal enzymes produced by lesser studied fungi, identification of research gaps, challenges in pursuing research in harnessing the potentials of marine fungi, and the scope for future prospects. Role of fungal enzymes in biogeochemical processes in marine environments, bioremediation, and plastic degradation is discussed indicating marine fungi as source of industrial enzymes. Scope for exploring marine fungal diversity and potentials of extremozymes, cold adaptive enzymes and halophilic fungal enzymes, besides the need for bioprocess development are discussed. Moreover, the challenges lying ahead in pursuing research on marine fungi are also discussed to draw the attention of mycologists and biotechnologists to appropriately harness the marine fungi.

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Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice BacteriumPseudoalteromonassp. AN178

Cited by 9 publications

References 30 publications

Marine-derived fungi: diversity of enzymes and biotechnological applications

Marine-derived fungi: diversity of enzymes and biotechnological applications

Crystal Structure of Wheat Glutaredoxin and Its Application in Improving the Processing Quality of Flour

Bioprospecting Marine Fungal Enzymes-Scope and Challenges

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