Cloning in companion animal, non-domestic and endangered species: can the technology become a practical reality?

Mastromonaco, Gabriela F.; King, W.A.

doi:10.1071/rd07034

Cited by 34 publications

(19 citation statements)

References 128 publications

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“…Dev. 58: [425][426][427][428][429][430][431] 2012) S ince the first cloned lamb was born, nuclear transfer (NT) has been challenging in several species and has produced many cloned offspring [1][2][3]. To produce cloned offspring, fetal fibroblasts have been chosen as a preferential donor cell line for NT so far because they have high proliferative potentials [4][5][6][7].…”

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confidence: 99%

Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells

Saadeldin

Choi

Torre

et al. 2012

Journal of Reproduction and Development

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Abstract. The temporal progressive increase of interferon tau (IFNτ) secretion from the bovine trophoblast is a major embryonic signal of establishing pregnancy. Here, we cultured and isolated bovine trophoblast cells (BTs) from IVM/IVF oocytes and in vitro produced blastocysts, used them, for the first time, as donor cells for nuclear transfer and compared them with adult fibroblasts (AFs) as donor cells. BTs were reprogrammed in enucleated oocytes to blastocysts with similar efficiency to AFs (14.5% and 15.6% respectively, P≤0.05). The levels of IFNτ, CDX2 and OCT4 expression in IVF-, BTand AF-derived blastocysts were analyzed using reverse transcription polymerase chain reaction and reverse transcription quantitative polymerase chain reaction (RT-PCR and RT-qPCR). IVF-produced embryos were used as reference to analyze the linear progressive expression of IFNτ through mid, expanded and hatching blastocysts. RT-PCR and RT-qPCR studies showed that IFNτ expression was higher in BT-derived blastocysts than IVF-and AF-derived blastocysts. Both IVF-and BT-derived blastocysts showed a progressive increase in IFNτ expression as blastocyst development advanced when it compared with AF-derived blastocysts. OCT4 was inversely related with IFNτ expression, while CDX2 was found to be directly related with IFNτ temporal expression. Persistence of high expression of IFNτ and CDX2 was found to be higher in BT-derived embryos than in IVF-or AF-derived embryos. In conclusion, using BTs expressing IFNτ as donor cells for bovine NT could be a useful tool for understanding the IFNτ genetics and epigenetics. Key words: Bovine trophoblast, Interferon tau, Nuclear transfer, Real-time PCR, Reprogramming (J. Reprod. Dev. 58: [425][426][427][428][429][430][431] 2012) S ince the first cloned lamb was born, nuclear transfer (NT) has been challenging in several species and has produced many cloned offspring [1][2][3]. To produce cloned offspring, fetal fibroblasts have been chosen as a preferential donor cell line for NT so far because they have high proliferative potentials [4][5][6][7]. In cattle, fetal and adult fibroblasts have been dominantly used for NT to produce cloned calves. Additionally, several types of cells, like granulosa, cumulus, oviduct epithelial cells, skin, tongue and other cells, have been used for NT [8,9].After fertilization of an egg with a sperm, the one-cell stage embryo grow up through several mitosis and reaches the preimplantation stage, becoming a blastocyst, which consists of an inner cell mass (ICM) that is capable of differentiation into all embryo organs and trophoblasts, which are the first differentiated cells from the embryo, and contributes formation of the placenta and fetal membranes but does not participate the formation of the fetus proper [7]. Some reports have demonstrated trophoblast isolation and its function in vitro in cattle [10][11][12]. In mice, living pups were born by nuclear transfer of trophectoderm cells from the expanded blastocysts into enucleated oocytes [13] as a trial to show...

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confidence: 99%

Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells

Saadeldin

Choi

Torre

et al. 2012

Journal of Reproduction and Development

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“…) and thus also necessary for embryo development. Previous studies on interspecies SCNT embryos have shown that poor developmental outcome may be attributed to disrupted communication between the nuclear and mitochondrial genomes of the different, albeit in most cases related, species (reviewed by Mastromonaco and King ). Establishment of xenomitochondrial homoplasmy, whereby an unrelated nucleus of one species interacts with the mitochondria of another, by Dey et al.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Development of Bison Embryos Using Interspecies Somatic Cell Nuclear Transfer

Alexander

et al. 2013

Reprod Domestic Animals

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Interspecies somatic cell nuclear transfer (interspecies SCNT) has been explored in many domestic and non-domestic animal species. However, problems arise during the development of these embryos, which may be related to species-specific differences in nuclear-cytoplasmic communication. The objectives of this study were to investigate the possibility of producing bison embryos in vitro using interspecies SCNT and assess the developmental potential of these embryos. Treatment groups consisted of cattle in vitro fertilization (IVF) and cattle SCNT as controls and wood bison SCNT, plains bison SCNT and wisent SCNT as experimental groups. Cleavage and blastocyst rates were assessed, and blastocyst quality was determined using total cell number, apoptotic incidence and relative quantification of mitochondria-related genes NRF1, MT-CYB and TFAM. These results indicate that embryos can be produced by interspecies SCNT in all bison species/subspecies (13.34-33.54% blastocyst rates). Although increased incidence of apoptosis was observed in bison SCNT blastocysts compared to cattle SCNT controls (10.45-12.69 vs 8.76, respectively) that corresponded with significantly lower cell numbers (80-87 cells vs >100 cells, respectively), no major differences were observed in the expression of NRF1, MT-CYB and TFAM. This study is the first to report the production of bison embryos by interspecies SCNT. Blastocyst development in all three bison species/subspecies was greater than the rates obtained in previous studies by IVF, which supports the potential role of SCNT for in vitro embryo production in this species. Yet, further investigation of developmental competence and the factors influencing blastocyst quality and viability is required.

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“…The species-specific nature of this organelle's biogenesis and utility makes it particularly relevant to functional iSCNT [30]. In concert with mitochondrial dysfunction, abnormal nuclear reprogramming is a major factor in failure of iSCNT to conserve endangered species [31].…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Takeda

Tasai

Iwamoto³

et al. 2012

Journal of Reproduction and Development

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Abstract. Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P < 0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.

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Cloning in companion animal, non-domestic and endangered species: can the technology become a practical reality?

Cited by 34 publications

References 128 publications

Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells

Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells

In Vitro Development of Bison Embryos Using Interspecies Somatic Cell Nuclear Transfer

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

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