Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin in<i>Pichia pastoris</i>

Paul, Rajkumar; Karthik, S.; Vimalraj, Ponnusamy; Meenakshisundaram, S.; Kaliraj, Perumal

doi:10.1080/10826068.2018.1514511

Cited by 6 publications

(2 citation statements)

References 67 publications

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“…Animals were split into four groups with six animals in each group. E-ALT-2, P-ALT-2 and P-TUFT-ALT-2 fusion protein were obtained from our lab (Paul et al, 2018b). One group was immunized intraperitoneally with 30 μg of P-TUFT-ALT-2 per animal in 100 μL of 0.5 M PBS along with equal volume of alum (1.3%) as adjuvant.…”

Section: Methodsmentioning

confidence: 99%

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

Paul

Ilamaran

Khatri

et al. 2019

Parasite Epidemiology and Control

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Introduction Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication. Methods An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in Pichia pastoris , a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT-ALT-2 in Balb/c mice. Result and discussion P-TUFT-ALT-2 showed an enhanced IgG peak titre compared to E. coli expressed E-ALT-2 and P. pastoris expressed P-ALT-2. IgG2b, IgG2a and IgG1 production were predominant indicating a balanced Th1/Th2 response. P-TUFT-ALT-2 also induced about 28% and 9.5% higher splenocyte proliferation over control and E-ALT-2 respectively. Splenocytes produced predominant IFN-γ followed by IL-5, IL-2 and IL-10 specifying a balanced Th1/Th2 response. P-TUFT-ALT-2 showed 55% to 80% with an average of 65% cytotoxicity in B. malayi L3 larvae in in vitro ADCC assay. Conclusion This experiment validates P-TUFT-ALT-2 as a potential vaccine candidate for human lymphatic filariasis.

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Section: Methodsmentioning

confidence: 99%

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

Paul

Ilamaran

Khatri

et al. 2019

Parasite Epidemiology and Control

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“…Unfortunately, alum did not promote the desired level of protection in non-human primates [37]. In an attempt to improve the vaccine-induced protection, some of the recent studies used analogs of TLR-4 or tuftsin to improve the protective immune responses and these studies demonstrated promising results [38,58,63]. In selecting the adjuvant, it is important to consider the fact that the potential end-users of the adjuvant will be the human.…”

Section: Adjuvant Formulations For Lf Vaccinementioning

confidence: 99%

Advances in Vaccine Development for Human Lymphatic Filariasis

Kalyanasundaram

Khatri

Chauhan

2020

Trends in Parasitology

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According to the World Health Organization, currently, over 880 million people are at risk of acquiring the lymphatic filariasis (LF) infection in over 52 countries worldwide. Current approaches to control LF by 2020 are reaching short of its anticipated goal. Several studies suggest the existence of protective immunity against LF in the human. Thus, it is possible to develop a prophylactic vaccine against LF infections in the human. Several potential vaccine candidates were identified and tested for their vaccine potential against LF. Preclinical studies to date suggest that it is possible to develop a prophylactic vaccine against LF. Much work needs to be done, but it is clear that a prophylactic vaccine combined with targeted chemotherapy is critically required for eliminating LF worldwide.

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In-silico development of multi-epitope subunit vaccine against lymphatic filariasis

Singh,

Shaikh,

Gupta

et al. 2023

Journal of Biomolecular Structure and Dynamics

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Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin inPichia pastoris

Cited by 6 publications

References 67 publications

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

Advances in Vaccine Development for Human Lymphatic Filariasis

In-silico development of multi-epitope subunit vaccine against lymphatic filariasis

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