Cloning, molecular characterization and functional analysis of a putative R2R3-MYB transcription factor of the phenolic acid biosynthetic pathway in S. miltiorrhiza Bge. f. alba

Hao, Ge‐Fei; Jiang, Xingyu; Feng, Lei; Tao, Ran; Li, Yanling; Huang, Luqi

doi:10.1007/s11240-015-0883-3

Cited by 30 publications

(32 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, SmMYB39 repressed phenolic acids biosynthesis when overexpressed in S. miltiorrhiza [ 17 ], whereas SmPAP1, the other member of MYB TFs, is involved in enhancing phenolic acid production by interacting with bHLH TFs SmMYC2 and activating genes encoding key enzymes in S. miltiorrhiza Bge. f. alba [ 18 ]. Zhou et al found JAZ-interacting TFs SmMYC2a and SmMYC2b positively regulate the biosynthesis of tanshinones and salvianolic acid B [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

Guo

Yang

et al. 2018

IJMS

View full text Add to dashboard Cite

Salvia miltiorrhiza Bunge is a Chinese traditional herb for treating cardiovascular and cerebrovascular diseases, and tanshinones and phenolic acids are the dominated medicinal and secondary metabolism constituents of this plant. WRKY transcription factors (TFs) can function as regulators of secondary metabolites biosynthesis in many plants. However, studies on the WRKY that regulate tanshinones and phenolics biosynthesis are limited. In this study, 69 SmWRKYs were identified in the transcriptome database of S. miltiorrhiza, and phylogenetic analysis indicated that some SmWRKYs had closer genetic relationships with other plant WRKYs, which were involved in secondary metabolism. Hairy roots of S. miltiorrhiza were treated by methyl jasmonate (MeJA) to detect the dynamic change trend of SmWRKY, biosynthetic genes, and medicinal ingredients accumulation. Base on those date, a correlation analysis using Pearson’s correlation coefficient was performed to construct gene-to-metabolite network and identify 9 SmWRKYs (SmWRKY1, 7, 19, 29, 45, 52, 56, 58, and 68), which were most likely to be involved in tanshinones and phenolic acids biosynthesis. Taken together, this study has provided a significant resource that could be used for further research on SmWRKY in S. miltiorrhiza and especially could be used as a cue for further investigating SmWRKY functions in secondary metabolite accumulation.

show abstract

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

Guo

Yang

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…In response, many studies have since focused on increasing this species phenolic acid contents by either overexpressing or suppressing its transcription factors or key enzyme genes within the relevant metabolic pathways. For example, the overexpression of SmTAT , SmC4H , SmHPPR , AtPAP1 , AtEDT1 , and SmPAP1 induced a substantial accumulation of phenolic acids in the transgenic S. miltiorrhiza ( Zhang Y. et al, 2010 ; Xiao et al, 2011 ; Hao et al, 2016 ; Liu Y. et al, 2016 ), whereas the downregulation of SmMYB39 , SmHPPD , SmCCR1 , or SmCHS all increased the content of phenolic acids ( Xiao et al, 2011 ; Wang et al, 2012 ; Zhang et al, 2013 ; Zhang et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

The Protein Kinase SmSnRK2.6 Positively Regulates Phenolic Acid Biosynthesis in Salvia miltiorrhiza by Interacting with SmAREB1

et al. 2017

View full text Add to dashboard Cite

Subclass III members of the sucrose non-fermenting-1-related protein kinase 2 (SnRK2) play essential roles in both the abscisic acid signaling and abiotic stress responses of plants by phosphorylating the downstream ABA-responsive element (ABRE)-binding proteins (AREB/ABFs). This comprehensive study investigated the function of new candidate genes, namely SmSnRK2.3, SmSnRK2.6, and SmAREB1, with a view to breeding novel varieties of Salvia miltiorrhiza with improved stress tolerance stresses and more content of bioactive ingredients. Exogenous ABA strongly induced the expression of these genes. PlantCARE predicted several hormones and stress response cis-elements in their promoters. SmSnRK2.6 and SmAREB1 showed the highest expression levels in the leaves of S. miltiorrhiza seedlings, while SmSnRK2.3 exhibited a steady expression in their roots, stems, and leaves. A subcellular localization assay revealed that both SmSnRK2.3 and SmSnRK2.6 were located in the cell membrane, cytoplasm, and nucleus, whereas SmAREB1 was exclusive to the nucleus. Overexpressing SmSnRK2.3 did not significantly promote the accumulation of rosmarinic acid (RA) and salvianolic acid B (Sal B) in the transgenic S. miltiorrhiza hairy roots. However, overexpressing SmSnRK2.6 and SmAREB1 increased the contents of RA and Sal B, and regulated the expression levels of structural genes participating in the phenolic acid-branched and side-branched pathways, including SmPAL1, SmC4H, Sm4CL1, SmTAT, SmHPPR, SmRAS, SmCHS, SmCCR, SmCOMT, and SmHPPD. Furthermore, SmSnRK2.3 and SmSnRK2.6 interacted physically with SmAREB1. In summary, our results indicate that SmSnRK2.6 is involved in stress responses and can regulate structural gene transcripts to promote greater metabolic flux to the phenolic acid-branched pathway, via its interaction with SmAREB1, a transcription factor. In this way, SmSnRK2.6 contributes to the positive regulation of phenolic acids in S. miltiorrhiza hairy roots.

show abstract

“…Recently, some MYB families have been determined to play important roles in wood formation and development in some tree species. For instance, AaMYB2 is involved in anthocyanin biosynthesis in anthurium spathes and leaves, while PtMYB4 from Pinus taeda was reported to regulate phenolic acid biosynthesis (Hao et al, 2016;. In addition, an R2R3 MYB TF MYB134 was found to be coinduced with PA biosynthetic genes in poplar after several kinds of stress treatments (Mellway et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco

Tian¹,

Sun²,

Bi³

2017

Turk J Biol

View full text Add to dashboard Cite

The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of stress-and ABA-related genes after NaCl treatment. These results indicated that FvMYB2 may play a positive role in salt stress regulation mediated by ABA-dependent signaling mechanisms.

show abstract

Cloning, molecular characterization and functional analysis of a putative R2R3-MYB transcription factor of the phenolic acid biosynthetic pathway in S. miltiorrhiza Bge. f. alba

Cited by 30 publications

References 42 publications

Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

The Protein Kinase SmSnRK2.6 Positively Regulates Phenolic Acid Biosynthesis in Salvia miltiorrhiza by Interacting with SmAREB1

A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco

Contact Info

Product

Resources

About