Cloning, nucleotide sequence, and overexpression of smoS, a component of a novel operon encoding an ABC transporter and polyol dehydrogenases of Rhodobacter sphaeroides Si4

Stein, Martin; Schäfer, Andreas; Giffhorn, Friedrich

doi:10.1128/jb.179.20.6335-6340.1997

Cited by 29 publications

(34 citation statements)

References 48 publications

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“…1B), separated merely by polS (encoding sorbitol dehydrogenase), as in R. sphaeroides Si4 (57). Thus, the ABC transporter might also be active with other polyols, e.g., sorbitol.…”

Section: Resultsmentioning

confidence: 99%

Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade

Wiegmann

Hensler

Wöhlbrand

et al. 2014

Appl Environ Microbiol

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Since genome analysis did not allow unambiguous reconstruction of transport, catabolism, and substrate-specific regulation for several important carbohydrates in Phaeobacter inhibens DSM 17395, proteomic and metabolomic analyses of N-acetylglucosamine-, mannitol-, sucrose-, glucose-, and xylose-grown cells were carried out to close this knowledge gap. These carbohydrates can pass through the outer membrane via porins identified in the outer membrane fraction. For transport across the cytoplasmic membrane, carbohydrate-specific ABC transport systems were identified. Their coding genes mostly colocalize with the respective "catabolic" and "regulatory" genes. The degradation of N-acetylglucosamine proceeds via N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate directly to fructose-6-phosphate; two of the three enzymes involved were newly predicted and identified. Mannitol is catabolized via fructose, sucrose via fructose and glucose, glucose via glucose-6-phosphate, and xylose via xylulose-5-phosphate. Of the 30 proteins predicted to be involved in uptake, regulation, and degradation, 28 were identified by proteomics and 19 were assigned to their respective functions for the first time. The peripheral degradation pathways feed into the Entner-Doudoroff (ED) pathway, which is connected to the lower branch of the Embden-Meyerhof-Parnas (EMP) pathway. The enzyme constituents of these pathways displayed higher abundances in P. inhibens DSM 17395 cells grown with any of the five carbohydrates tested than in succinate-grown cells. Conversely, gluconeogenesis is turned on during succinate utilization. While tricarboxylic acid (TCA) cycle proteins remained mainly unchanged, the abundance profiles of their metabolites reflected the differing growth rates achieved with the different substrates tested. Homologs of the 74 genes involved in the reconstructed catabolic pathways and central metabolism are present in various Roseobacter clade members.

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“…1B), separated merely by polS (encoding sorbitol dehydrogenase), as in R. sphaeroides Si4 (57). Thus, the ABC transporter might also be active with other polyols, e.g., sorbitol.…”

Section: Resultsmentioning

confidence: 99%

Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade

Wiegmann

Hensler

Wöhlbrand

et al. 2014

Appl Environ Microbiol

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“…ABC-T SMc01496 (CUT1) was induced by the C6 polyols dulcitol, sorbitol, mannitol, and maltitol (Glu␣1-4sorbitol) but not by sugars. It has high identity to the Smo system of Rhodobacter sphaeroides (28), with genes encoding a putative sorbitol dehydrogenase (smoS; SMc01500) and mannitol dehydrogenase (mtlK; SMc01501) downstream of SMc01496.…”

Section: Site the Results Are Shown In Full Inmentioning

confidence: 99%

Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome

Mauchline

Fowler

East

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

138

146

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The number of solute-binding protein-dependent transporters in rhizobia is dramatically increased compared with the majority of other bacteria so far sequenced. This increase may be due to the high affinity of solute-binding proteins for solutes, permitting the acquisition of a broad range of growth-limiting nutrients from soil and the rhizosphere. The transcriptional induction of these transporters was studied by creating a suite of plasmid and integrated fusions to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to Ϸ47% of the ABC uptake systems and 53% of the TRAP transporters in S. meliloti. Of these transport systems, 64 are previously uncharacterized in Rhizobia and 24 were induced by solutes not known to be transported by ABC-or TRAP-uptake systems in any organism. This study provides a global expression map of one of the largest transporter families (transportome) and an invaluable tool to both understand their solute specificity and the relationships between members of large paralogous families.ATP-binding cassette ͉ expression ͉ high throughput ͉ transport ͉ tripartite ATP-independent periplasmic

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“…While mannitol is a very good carbon source for S. coelicolor, growth on sorbitol is barely detectable. A gene cluster on cosmid SCI7 was found that could encode a permease (50). SmoD exhibits 30% identity to GutB, a sorbitol dehydrogenase present in Bacillus subtilis (27).…”

Section: Resultsmentioning

confidence: 99%

In Silico and Transcriptional Analysis of Carbohydrate Uptake Systems of Streptomyces coelicolor A3(2)

et al. 2004

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Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes. As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutritional state of the cell and, therefore, influence morphogenesis and antibiotic production. We have surveyed the genome of S. coelicolor A3(2) to provide a thorough description of the carbohydrate uptake systems. Among 81 ATP-binding cassette (ABC) permeases that are present in the genome, we found 45 to encode a putative solute binding protein, an essential feature for carbohydrate permease function. Similarity analysis allowed the prediction of putative ABC systems for transport of cellobiose and cellotriose, ␣-glucosides, lactose, maltose, maltodextrins, ribose, sugar alcohols, xylose, and ␤-xylosides. A novel putative bifunctional protein composed of a substrate binding and a membrane-spanning moiety is likely to account for ribose or ribonucleoside uptake. Glucose may be incorporated by a proton-driven symporter of the major facilitator superfamily while a putative sodium-dependent permease of the solute-sodium symporter family may mediate uptake of galactose and a facilitator protein of the major intrinsic protein family may internalize glycerol. Of the predicted gene clusters, reverse transcriptase PCRs showed active gene expression in 8 of 11 systems. Together with the previously surveyed permeases of the phosphotransferase system that accounts for the uptake of fructose and N-acetylglucosamine, the genome of S. coelicolor encodes at least 53 potential carbohydrate uptake systems.Streptomycetes represent a major fraction of the bacterial soil population (20). They contribute substantially to carbon recycling by degrading a whole variety of biopolymers that stem from dead plant and animal material (16). These organic compounds, which include xylan, chitin, and cellulose, are broken down by exoenzymes. The products are funneled into the cell by specific carbohydrate importers that usually recognize mono-and disaccharides. The recent publication of the genome of the model organism Streptomyces coelicolor A3(2) revealed two interesting features concerning carbon utilization (7), that is, (i) a huge number of 172 genes encoding secreted proteins, such as hydrolases, chitinases, cellulases, lipases, nucleases, and proteases, and (ii) the occurrence of 81 ATPbinding cassette (ABC) permeases that are possibly used for the uptake of sugars, oligopeptides, and nucleosides as well as for drug export (46, 56). The numerousness of exoenzymes and ABC systems is 5-to 10-fold higher than that of other bacteria, underlining the broad metabolic capacity of streptomycetes (7).Canonical carbohydrate-specific ABC systems in gram-positive bacteria are oligoprotein assemblies: a membrane-anchored substrate-binding protein is exposed to the outside of the cell, scavenging for a specific substrate molecule (10) (Fi...

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Cloning, nucleotide sequence, and overexpression of smoS, a component of a novel operon encoding an ABC transporter and polyol dehydrogenases of Rhodobacter sphaeroides Si4

Cited by 29 publications

References 48 publications

Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade

Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade

Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome

In Silico and Transcriptional Analysis of Carbohydrate Uptake Systems of Streptomyces coelicolor A3(2)

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